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OfflineGizmoleon
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Sfd's to restrictive?
    #23756877 - 10/21/16 03:17 AM (7 years, 3 months ago)

Hi! I couldn't find any info on the mather so I have to ask. I recently bought some 0,2 micron sfd's from a reputable vendor but they seem extremely restrictive. The silicone I used on my lids actually ripped from the pressure under pc'ing. I didn't use the high temperature stuff but some other silicone which can witstand temps up to 130C. I know this probably is the reason it gave in, but is it really supposed to be so restrictive that one cant blow trough it? The type i bought is paper thin btw. I know tyvek is very restrictive too, but i makes me wonder. Has anyone experience with this kind of sfd's?


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"Who says it's a Camberwell Carrot?
I do. I invented it in Camberwell, and it looks like a carrot."


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InvisibleJosex
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Re: Sfd's to restrictive? [Re: Gizmoleon]
    #23756893 - 10/21/16 03:28 AM (7 years, 3 months ago)

I also have sfd's that are paper thin and they have a pore size of 0.22um. If you could blow through them that'd mean bad news, so yeah it's normal that they are that restrictive.

I'm not sure if yours are the same as mine. The ones I have may be thin but they are thin because they are efficient. They have a hydrophobic membrane, so they can not get wet or absorb water which means bacteria can not transverse the filter. They are made from polytetrafluoroethylene which means they are chemically inert and will not be degraded by heat.

These filter have come from a research lab where they were originally ordered in from Sigma-Aldrich, who are a market leaders in supplying research chemicals and products to research labs and universities throughout the UK and Europe. You will not find the thick synthetic filters there because they only work due to the thickness and are not made to any such standards.

Use rtv silicone.


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OfflineGizmoleon
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Re: Sfd's to restrictive? [Re: Josex]
    #23756913 - 10/21/16 03:44 AM (7 years, 3 months ago)

Thanks for the quick reply. Yeah, I suppose it wouldn't be good if you could blow to them, but they just seemed totally clogged. I guess their not, coming from a mushrom supply and all. I have gotten a new supply of rtv silicone so i'll give it a try with that.

Btw, I'm planning on inoculate som rye jars with isolated agar growth. Tic tac toe style. However this agar is the third transfer. It's clean and growth is fast and healthy, but it's only partly rizomorphic and still has lots of sectors. Is it okay to innoc with it anyways as long as it's clean or should i do more transfers to I see clean rizomorphic growth. I'm a little eager to get started cause my last supply of mushrooms just got eaten..


--------------------
"Who says it's a Camberwell Carrot?
I do. I invented it in Camberwell, and it looks like a carrot."


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Invisibleblindingleaf
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Re: Sfd's to restrictive? [Re: Josex]
    #23756914 - 10/21/16 03:44 AM (7 years, 3 months ago)

use coarse sandpaper to roughen up the plastic around the GE hole of ur lid before applying silicone or RTV (both work) so that it adheres better.  with flat shiny plastic, its going to have hard time staying put.  after u apply the small circle to the silicone, make sure the excess isn't spilling into where ur GE hole is because that will restrict it further.

non vendor spoiler
fungi perfecti has best prices for SYNTHETIC filter discs

aloha has best prices for CELLULOSE filter discs

myco supply has good ones too, just not as cheap in bulk.

i never used or ordered outside of those guys for filters, but if its a vendor here it should be ok.


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MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlineenlightenment
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Re: Sfd's to restrictive? [Re: Gizmoleon]
    #23756922 - 10/21/16 03:50 AM (7 years, 3 months ago)

Inoculate right now.
Rizomorphic mycelium does not guarantee that is a great fruiter or good in potency or something. It is possible to isolate a strain from spores which has bad genetics and would never fruit but has awesome rhizomorphic growth.


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InvisibleJosex
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Re: Sfd's to restrictive? [Re: Gizmoleon]
    #23756940 - 10/21/16 04:03 AM (7 years, 3 months ago)

Quote:

Gizmoleon said:

Btw, I'm planning on inoculate som rye jars with isolated agar growth. Tic tac toe style. However this agar is the third transfer. It's clean and growth is fast and healthy, but it's only partly rizomorphic and still has lots of sectors. Is it okay to innoc with it anyways as long as it's clean or should i do more transfers to I see clean rizomorphic growth. I'm a little eager to get started cause my last supply of mushrooms just got eaten..




If the growth is fast and healthy the plate is good to go, you can def use it to inoculate your jars. You don't need to worry about sectoring or rhizomorphs, unless your goal is to get an isolated strain, which would take far more transfers that just three.

If you use that plate to noc your jars it'd still be a MS grow, a lil' bit narrowed down if it's a third transfer and you'll get the benefits of a MS grow.

If that's the only plate you have ready, I wouldn't use the tic-tac-toe because that tek is normally used to noc one jar with one plate.

You can use that plate to make an LI and this will allow you to noc a bunch of jars easily.


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OfflineGizmoleon
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Re: Sfd's to restrictive? [Re: Josex]
    #23757532 - 10/21/16 10:02 AM (7 years, 3 months ago)

I have six plates ready. Gonna use 4 for ttt in quart jars with rye, one for an blenderless slurry experiment and one for transferring to more plates.

I have a litte trouble understanding the "viability cycle" of mycellium. To my limited knowledge I have to start from scratch with spores or cloning(?) after three to four grows from the same master? I were reading an article about this, but I cant find it. Do I make myself understandable?:blush:

Thank you so much for your help btw. Much appreciated.


--------------------
"Who says it's a Camberwell Carrot?
I do. I invented it in Camberwell, and it looks like a carrot."


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InvisibleJosex
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Re: Sfd's to restrictive? [Re: Gizmoleon]
    #23758060 - 10/21/16 01:20 PM (7 years, 3 months ago)

Quote:

Gizmoleon said:
I have six plates ready. Gonna use 4 for ttt in quart jars with rye, one for an blenderless slurry experiment and one for transferring to more plates.

I have a litte trouble understanding the "viability cycle" of mycellium. To my limited knowledge I have to start from scratch with spores or cloning(?) after three to four grows from the same master? I were reading an article about this, but I cant find it. Do I make myself understandable?:blush:

Thank you so much for your help btw. Much appreciated.




Agar has several uses in mycology; cleaning, isolation and storage of cultures, cloning of live tissue and germination of spores, and it's also used to expand cultures. The latter seems to be the one you need clarification on.

There are several ways to expand a given culture and keep it indefinitely but you gotta have a couple of basic things in mind; always keep a back-up plate of that culture (short-term storage) or a master slant (long-term storage). You need a back-up culture in order to avoid "senescense" which is basically a drop-off in performance that occurs when you let said culture undergo a lot of celular division.
An instance of senescence could be seen after a protracted expansion of a given culture using the method G2G (grain to grain).

For example, you use a wedge from the master culture to noc a grain jar. This jar would be G1 (generation 1) and in turn you could use this jar to noc a number of jars (G2). G2 jars can noc a batch of G3 jars... Your spawn can grow exponentially this way. This process can continue until eventually you hit senescence and you'd be forced to go back to the master culture to restart the whole process.

You could go about this in several ways and the following is just an example. Since you don't know yet if your cultures are good performers, I'd wait until you grow them out. You could clone fat pins from nice clusters and put them on agar, clean them (by transfering clean tissue to more plates) and once you have a clean culture, be sure to keep a back-up plate of every clone. You need to test them out, keep the ones that perform well and discard the poor performers. You can keep the cultures in the fridge until you test them out and it's time to decide which one you wanna keep.

Once you have a winner, you could expand that plate into more plates (but keep the original at least, although you can use the original plate to create more back-ups of that culture). Every one of those plates could be used to noc some master jars that you could use to further expand your spawn via G2G. Once you've expanded the culture to G3 or G4 there's no need to continue and it'd be better to restart the process.

Master slants can keep a culture in the fridge for up to two years or more, so if you got a badass culture that's something you want to keep in mind.


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OfflineGizmoleon
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Re: Sfd's to restrictive? [Re: Josex]
    #23766345 - 10/24/16 10:15 AM (7 years, 3 months ago)

Deleted: Double post


--------------------
"Who says it's a Camberwell Carrot?
I do. I invented it in Camberwell, and it looks like a carrot."


Edited by Gizmoleon (10/24/16 10:19 AM)


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OfflineGizmoleon
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Re: Sfd's to restrictive? [Re: Josex]
    #23766347 - 10/24/16 10:16 AM (7 years, 3 months ago)

Thank you for a excellent explanation:smile: I've been a little preoccupied so I haven't had much time online, but the jars are now inoculated ttt style and so far so good. Three days since the drop and the grains closest to the slants are turning bright white. I had every single nine slants sticking to the glass in one jar though. Sticky little devils. In worst case scenario it will only take a little more time.


Edited by Gizmoleon (10/24/16 10:17 AM)


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Invisibler.lutece
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Re: Sfd's to restrictive? [Re: Gizmoleon]
    #23766378 - 10/24/16 10:34 AM (7 years, 3 months ago)

Quote:

For example, you use a wedge from the master culture to noc a grain jar. This jar would be G1 (generation 1) and in turn you could use this jar to noc a number of jars (G2). G2 jars can noc a batch of G3 jars... Your spawn can grow exponentially this way. This process can continue until eventually you hit senescence and you'd be forced to go back to the master culture to restart the whole process.





It should be noted that though these are referred to as "generations" for our sake, they are in fact all the same organism. The senescence referred to basically means less healthy/viable mycelium as a result of old age (much in the way humans are less healthy at old age). This is because through these kinds of transfers, the same organism is kept alive for a long time. The reason master plates and slants are refrigerated is to slow down this process, keeping the mycelium healthy for a longer period. I think I remember reading that mycelium can thrive up to 6 months at room temp, but is best used before 90 days of not being refrigerated. (Keep in mind that refrigerated days aren't counted towards the mycelium's age.)


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One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineGizmoleon
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Re: Sfd's to restrictive? [Re: r.lutece]
    #23768624 - 10/24/16 11:24 PM (7 years, 3 months ago)

Regarding spores to agar I have three attempts so far with no results. Although the agar recipe has been the same every time I have tried two different syringes/strains (GT and Ecuador) and variable amounts of solution. These attempts were all done with no pour pastyplates and problems with condensation so this could be the reason. Next time I will try with a print and loop. Maybe less agar too as I understand a more moist environment is better for spores. Yeast, activated charcoal? As things progress now I don't really need it to work, but it would have been nice mastering the technique.

After I started with petris and pouring both condensation and contams has reduced drastically. Actually, in about twenty transfers I had one bacterial contam. I really love my new hobby! Such a shame it's illegal in my parts. Almost everything I have learned I have to thank this forum for. I started lurking here in june and was overwhelmed, but equally intrigued. So far it's only been one successful pf-grow and another on the way. One total failure though, with me beeing too eager with spores to grain after two xanax. Oh man what a mess, but I learned alot on that one. I'll be back with more questions when my grains are done, hopefully.


--------------------
"Who says it's a Camberwell Carrot?
I do. I invented it in Camberwell, and it looks like a carrot."


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