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globos
Regular User


Registered: 04/09/10
Posts: 658
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1st flush! Now I'd like to make spore prints [Re: ComebackKid]
#23858310 - 11/22/16 06:43 PM (7 years, 2 months ago) |
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I'm so happy! Here's the first flush. I only harvested the two trays that look more full.
Apart from the obvious huge satisfaction for my first decent harvest, I have to say there are a few glitches. One tray had already produced spores. The fruits are not as big as I'm used to with the growkits. I guess reduced fruit size might be due to the clone I started it all from. It was a first-flush late fruit that had already produced spores.
I tried to make a couple spore prints from this flush, but I'm not entirely confident I got it right. What do you think could be the easiest, newbie-proof tek for spore prints? It will be my insurance against senescence. Besides, I can't wait to begin trading and/or giving stuff away! 
DAY 27 (FRUITING DAY 13)

EDIT On harvesting, some thanks are due. First and foremost, the guys who offered most of the help in my first try - @SpitballJedi and @MycoFanatikos. Then Pastywhyte with his foolproof agar tek! Without that, I couldn't have achieved anything. Then the guys who helped the most in my second grow (this thread) - @ComebackKid, @bodhisatta and @Mushierage. THANK YOU!
I'm not saying goodbye yet - I will keep updating this thread until I throw the substrate in my compost pile :-)
Edited by globos (11/22/16 07:14 PM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: 1st flush! Now I'd like to make spore prints [Re: globos]
#23858435 - 11/22/16 07:18 PM (7 years, 2 months ago) |
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Looking good! Congratulations!
-------------------- Keep your feet on the ground and your head in the clouds
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: 1st flush! Now I'd like to make spore prints [Re: Shineonyoucrazy]
#23858766 - 11/22/16 09:03 PM (7 years, 2 months ago) |
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Right on man!

Just chop off the caps off the mushrooms with scissors or a knife and place on foil in your SAB Leave for ~24 hours then fold some foil over the print and fold/seal the edges
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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khatinthehat
Stranger
Registered: 11/22/16
Posts: 4
Last seen: 7 years, 2 months
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Re: 1st flush! Now I'd like to make spore prints [Re: globos]
#23858927 - 11/22/16 10:22 PM (7 years, 2 months ago) |
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looking good, im glad to be back in the game. Posts like this make me wonder why i ever stopped.
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globos
Regular User


Registered: 04/09/10
Posts: 658
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Re: 1st flush! Now I'd like to make spore prints [Re: khatinthehat]
#23875119 - 11/28/16 12:37 PM (7 years, 2 months ago) |
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I took some prints on foil, but only 3 came out pretty and dark. I doubt they're sterile, but I guess that's how it goes, some cleaning will be needed when they go on agar. I'll take more prints as soon as I have more fruits, speaking of which...
The second flush is lagging. Still no macroscopic sign of life. Is that normal?
The jars I inoculated 17 days ago also show little to no sign of life. This time I just cut up the agar and dropped pieces into the jars. In the past, I used to use some sterile water to wash up the mycelium and inject it with a syringe. Liquid inoculation did cause contamination in some cases, but on the other hand the colonization was pretty fast. I wonder.
Any advice on these issues?
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globos
Regular User


Registered: 04/09/10
Posts: 658
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Re: Life goes on (V3) [Re: globos]
#23890995 - 12/03/16 03:14 PM (7 years, 1 month ago) |
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2nd flush coming but quite slowly. Fat fruits, fatter than 1st flush. I guess they'll also get taller.
As for the jars that will make my next grow, there might be some sign of life, but they're still very slow. I wonder if that's normal. Advice anyone?
Quote:
globos said:

Quote:
globos said: The jars I inoculated 17 days ago also show little to no sign of life. This time I just cut up the agar and dropped pieces into the jars. In the past, I used to use some sterile water to wash up the mycelium and inject it with a syringe. Liquid inoculation did cause contamination in some cases, but on the other hand the colonization was pretty fast. I wonder.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Life goes on (V3) [Re: globos]
#23891166 - 12/03/16 04:17 PM (7 years, 1 month ago) |
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Some people use grain waterb but that sounds pretty nasty. I know it works but its not really ideal for my applications. My only hardship in cultivation is preventing bacteria from forming in grain jars, definitely dont want to make an lc out of it. I just shake up an agar puck in 100 ml of water and pour in the jars. (Thanks munch). So much cleaner.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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globos
Regular User


Registered: 04/09/10
Posts: 658
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Second flush [Re: globos]
#23917064 - 12/11/16 05:17 PM (7 years, 1 month ago) |
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Second flush complete, all 4 trays. Speed varied significantly among different trays, while yield was more or less the same.
DAY 52 (FRUITING DAY 38) - Second Flush Trays 1&2
 The two quickest trays 1-2 days before harvesting. Sorry for the pics - very low quality. I took them in a hurry - the shitty phone had a scratched lens too.
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DAY 57 (FRUITING DAY 43) - Second Flush Trays 3&4
 The two slower trays together on the day of harvest. Differences in color and shape are easy to see even through the bags. One tray has caps that tend to rip, the other has darker caps spotted with white.
 The differences are easier to see with close individual shots. From above ...
 ... and from the side.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Second flush [Re: globos]
#23917126 - 12/11/16 05:34 PM (7 years, 1 month ago) |
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Man that sucks. Better luck next time
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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globos
Regular User


Registered: 04/09/10
Posts: 658
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Re: Second flush [Re: Kenetic]
#23935295 - 12/17/16 12:29 PM (7 years, 1 month ago) |
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I'm not complaining about the second flush, since the first was satisfactory. I've spread much love and gave about 1/3 of the product to the friend who helped the most; more will be given out during the holiday season, and there will plenty left for me - so it's all right!
I'm a little worried about V3 though. The jars I inoculated exactly one month ago don't look like they are colonising much - or at all. That's too long, isn't it?
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Second flush [Re: globos]
#23935355 - 12/17/16 12:50 PM (7 years, 1 month ago) |
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For me it is
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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