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Nighted
Ghost


Registered: 11/09/01
Posts: 698
Loc: Funkytown
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Re: What do you use as agar bottle? [Re: bodhisatta]
#25268497 - 06/14/18 06:18 PM (5 years, 7 months ago) |
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Quote:
bodhisatta said: a quarter of the way 
you can fill that up to 450mL no problem.
Nice GIF...
Read what I said. You can and you can't. It depends on the viscosity of the medium. This ain't rocket science man.
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 Freedom defined is freedom denied. Nighted is better than Google. Please take time to rate 5/5. Thanks!
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Chilean6
Stranger



Registered: 05/04/18
Posts: 17
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Re: What do you use as agar bottle? [Re: the.raven]
#25271103 - 06/15/18 10:03 PM (5 years, 7 months ago) |
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Quote:
the.raven said:
... I like to prepare agar (mix and PC) ahead of time, refrigerate, and reheat when needed. I typically make 500mL (10g Agar, 7.5g malt extract, 1g yeast if at all, ...
I didn't think it was okay to do this, I thought you *must pour* your plates at the exact moment your agar cools to the perfect temperature and then you can store the *plates for later use (okay I'm exaggerating a little).
Can you explain how long you store the prepared agar for and what your process is for pouring it once you take it out of the refrigerator?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: What do you use as agar bottle? [Re: Chilean6]
#25271125 - 06/15/18 10:21 PM (5 years, 7 months ago) |
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i use a captain morgan handle with the lid drilled out and a sfd rtv'ed in. i almost always pour the whole liter at once and use the plates as i need them. but, you can reheat, whether by water bath or microwave, iso the bottle well and pour as normal. it'll actually stay viable for a very long time if you can keep it clean.
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the.raven
it learns


Registered: 11/01/17
Posts: 62
Last seen: 2 years, 2 months
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Re: What do you use as agar bottle? [Re: Chilean6]
#25271429 - 06/16/18 03:45 AM (5 years, 7 months ago) |
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If I intend to pour that night...
I wash my hands and place my stack of petris in the SAB while I wait for the PC to come to pressure/temp. After the PC cools, I remove the bottles and immediately tighten the lids down. I wipe the bottle down with iso, place it in my SAB, turn the HEPA filters on high, and take a shower while I wait for the agar to reach pouring temp.
If I intend to store...
After the PC cools, I remove the bottles and immediately tighten the lids down. Place bottles on a wire cooling rack until room temp, wipe bottles down with iso, place in culture refrigerator.
My culture fridge usually hovers around 4C (40F), 70%RH. I'm unsure on maximum cold storage time without contamination. I pour plates fairly often, I've stored prepared bottles for about 2-3 months (without issue).
To reheat, I remove the foil, LOOSEN THE CAP (enough to relieve pressure as the agar heats, the penalty is blowing a cap off and cleaning a microwave), and set it in a clean microwave. This is the boring part, babysit the bottle as it reheats. Watch closely, it will boil over. Reheat until agar mix is fully liquid state.
I have tried waterbath and microwave reheating. In my experience, microwaving is tedious (a lot of watching and waiting) but less time consuming overall.
My agar is usually liquid at about 87C+, I pour a 20 plate stack around 47-50C. If I pour a large stack around that temp range I tend to see less condensation on the lids. I use an IR thermometer to measure bottle temp. I would like to buy or make some GL45 caps that will fit a thermocouple (more accurate measurements) but it's kinda unnecessary and only seeks to satisfy my nerdiness.
In the past, when planning to store, I have recorded cooling rates for the agar bottles. (You can use Newton's Law of Cooling to estimate cooling rates but expect some error in the model) I measured temps immediately after removing from the PC, every 5 minutes after, and graphed the results in Excel. When I plan to pour, I measure the bottle temp and reference the spreadsheet data to estimate when the agar will be at pouring temp. I set an alarm so I can walk away and work on other tasks while I wait.
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soto5

Registered: 01/28/18
Posts: 10
Last seen: 5 years, 6 months
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Re: What do you use as agar bottle? [Re: the.raven]
#25272253 - 06/16/18 01:17 PM (5 years, 7 months ago) |
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If you're cheap like me, look for these 24cm tall (9.5 lengths of 3 barleycorns) Buchsteiner shaker bottles. They can be available in pretty much any store that sells protein powder. Both the body and the one piece lid are PP5 and it has a nice long spout for pouring. The nominal capacity is 700ml and it also has markings for every 50ml. It should cost you anywhere from 0-5€ (unless you're getting ripped off).
 (ignore the label, almost every supplier has their own logo printed on these) Avoid these newer ones that have a different top, the detachable hinged stopper isn't PP5, I learned the hard way.
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Ama420
Thickems



Registered: 04/12/17
Posts: 1,159
Last seen: 2 years, 2 months
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Re: What do you use as agar bottle? [Re: soto5]
#25272560 - 06/16/18 03:49 PM (5 years, 7 months ago) |
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 500ml pyrex media bottle is the best hands down. I fill mine to 500ml everytime. MEA and never have boil over. Follow bods tek it works perfect
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
elasticaltiger said: Fungi perfecti recommended filling media flasks to the 50% mark.
Paul
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NarkedAt90ft
Deep in the Blue Abyss


Registered: 12/04/13
Posts: 149
Loc: Marianas Trench
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Re: What do you use as agar bottle? [Re: bodhisatta]
#25273107 - 06/16/18 08:04 PM (5 years, 7 months ago) |
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AliExpress (alibaba app i think?) anyway Aliexpress has quite a few cheap media bottle sellers..
Trick is to find sellers that sell the bottle really cheap and charge a little more for shipping and order a couple. Sometimes the shipping inflates, sometimes it doesn't, key is to find a seller that doesnt inflate the shipping until you have 3 or 4 in your cart. I picked up 2 500ml shipped for what would equate to maybe $10 American probably less.
Where I am 1 Pyrex 500ml media bottle shipped was going to run me $20+ American, so this was a great deal for me. Maybe it'll work out for others.
They list as reagent bottles, and the ones with the blue caps have the perfect no drip pour rings. I love the no drip rings. The are awesome.
-------------------- ni·tro·gen nar·co·sis noun - a drowsy state induced by breathing air under higher than atmospheric pressure, for example, in deep-sea diving. Also known as Rapture of The Deep. Let's Trade!
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rockpup
Old skater punk



Registered: 03/02/18
Posts: 312
Loc: Everywhere or anywhere
Last seen: 4 years, 3 months
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Re: What do you use as agar bottle? [Re: NarkedAt90ft]
#25273387 - 06/16/18 10:44 PM (5 years, 7 months ago) |
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Yeah dude mine was cheap on ebay. I looked for ever because I am fucking cheap. $11.95 canuck shipped-They are $13.95 now I just checked "Reagent Glass Bottle 500ml - Borosilicate - Blue Screw Cap" not sure if i can list shit form ebay or not-but search that line in ebay sure you will find it.Shipped fast I must say for chinaland.
-------------------- SKATE OR DIE! RIP 1989-2001-The best of time's I miss molly.
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k5hd2y
Depersonalized & Complete


Registered: 04/13/17
Posts: 675
Last seen: 4 years, 2 months
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Re: What do you use as agar bottle? [Re: rockpup]
#25273447 - 06/16/18 11:21 PM (5 years, 7 months ago) |
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1.5 pint mason jar and a small glass milk bottle.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 1 day, 2 hours
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Re: What do you use as agar bottle? [Re: k5hd2y]
#25273604 - 06/17/18 02:03 AM (5 years, 7 months ago) |
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Soy sauce bottle is good for a small batch and fits in the microwave.
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Chilean6
Stranger



Registered: 05/04/18
Posts: 17
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Re: What do you use as agar bottle? [Re: Kizzle]
#25274422 - 06/17/18 12:12 PM (5 years, 7 months ago) |
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Quote:
Kizzle said: Soy sauce bottle is good for a small batch and fits in the microwave.

how do you do your cap?
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Re: What do you use as agar bottle? [Re: Nighted]
#25398587 - 08/19/18 01:32 PM (5 years, 5 months ago) |
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i used a 750ml gin bottle lol worked fine for me. just propped it up sideways with a jar in my pressure cooker. Fuck agar is alot of work though would rather roll the dice on 5 tubs of MS lol
Edited by numnum59 (08/19/18 01:33 PM)
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Re: What do you use as agar bottle? [Re: numnum59]
#25399647 - 08/19/18 10:26 PM (5 years, 5 months ago) |
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Wine bottle all the way, never had problems, never had contams. I mean never had contams b/c of the pouring vessel.
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