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Offlinemushhiehunter
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What do you use as agar bottle?
    #23754979 - 10/20/16 01:58 PM (7 years, 3 months ago)

What are suitable "agar bottles" for pouring mind you I'm in europe so "get it in walmart" is not a viable option :smile: And is the polyfill the best option or should I make a special tyvek bottle cap?


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Offlinenumnum59
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Re: What do you use as agar bottle? [Re: mushhiehunter]
    #23754990 - 10/20/16 02:02 PM (7 years, 3 months ago)

I just started agar yesterday, i used a 750mL vodka bottle and it worked perfectly. I also used polyfil with aluminum foil wrapped over it, this way i can keep the cap to help agitate the agar when i first mix it.





Edited by numnum59 (10/20/16 02:11 PM)


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Offlinemushhiehunter
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Re: What do you use as agar bottle? [Re: numnum59]
    #23755051 - 10/20/16 02:23 PM (7 years, 3 months ago)

Wine bottle it is... :cool:


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Offlinenumnum59
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Re: What do you use as agar bottle? [Re: mushhiehunter]
    #23755065 - 10/20/16 02:27 PM (7 years, 3 months ago)

i would recommend a vodka or gin bottle...













... purely because they are better to drink! :P


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Offlinemushhiehunter
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Re: What do you use as agar bottle? [Re: numnum59]
    #23755096 - 10/20/16 02:36 PM (7 years, 3 months ago)

Quote:

numnum59 said:
i would recommend a vodka or gin bottle...


Too bad I don't drink hard liquor...










... purely because they are better to drink! :P




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Offlinemushhiehunter
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Re: What do you use as agar bottle? [Re: numnum59]
    #23755117 - 10/20/16 02:44 PM (7 years, 3 months ago)

Quote:

numnum59 said:
I just started agar yesterday, i used a 750mL vodka bottle and it worked perfectly. I also used polyfil with aluminum foil wrapped over it, this way i can keep the cap to help agitate the agar when i first mix it.








Huh, i read your advice once more and I realised one thing, why would you want to agitate the agar to mix it? I like to first boil it stirring it well in a pot and then transfer it into the bottle, is it just to skip this step or is there another purpose to it? And also, when do you pour the agar? Just when the bottle is cool enough to touch or you wait till the agar just starts solidifying?


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Offlinenumnum59
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Re: What do you use as agar bottle? [Re: mushhiehunter]
    #23755145 - 10/20/16 02:53 PM (7 years, 3 months ago)

Quote:

mushhiehunter said:


Huh, i read your advice once more and I realised one thing, why would you want to agitate the agar to mix it? I like to first boil it stirring it well in a pot and then transfer it into the bottle, is it just to skip this step or is there another purpose to it? And also, when do you pour the agar? Just when the bottle is cool enough to touch or you wait till the agar just starts solidifying?




so i follow RR's agar video, except the cap and the recipe. i like mixing it in the bottle because its less messy.


and when i poured the agar yesterday i fucked up because it was cool but it condensed a bunch on my lids. next time i will be following this tek...
https://www.shroomery.org/forums/showflat.php/Number/22085395#22085395

his way of cooling it down is brilliant, i wish i would have read that yesterday.


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InvisiblebodhisattaMDiscordReddit
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Re: What do you use as agar bottle? [Re: numnum59]
    #23755166 - 10/20/16 03:01 PM (7 years, 3 months ago)

I use a bottle made for agar for agar.
Agar link in signatur first link you find there


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InvisibleMycolorado
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Re: What do you use as agar bottle? [Re: bodhisatta]
    #23755171 - 10/20/16 03:02 PM (7 years, 3 months ago)

Mason jar.


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InvisiblebodhisattaMDiscordReddit
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Re: What do you use as agar bottle? [Re: Mycolorado]
    #23755186 - 10/20/16 03:07 PM (7 years, 3 months ago)

Mason jar sucks dick for agar


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InvisibleChilean6
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Re: What do you use as agar bottle? [Re: bodhisatta]
    #25268280 - 06/14/18 04:26 PM (5 years, 7 months ago)

Quote:

bodhisatta said:
Mason jar sucks dick for agar




Other than spending $$$ on a media bottle (not cheap where I come from) and the wide mouth for pouring, why's that?


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InvisibleChilean6
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Re: What do you use as agar bottle? [Re: Chilean6]
    #25268284 - 06/14/18 04:27 PM (5 years, 7 months ago)

Oops wasn't paying attention to time of last post... is there a suggestion for timeframe of it still being acceptable to reply to an old thread?

I guess its still a valid question.


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InvisiblebodhisattaMDiscordReddit
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Re: What do you use as agar bottle? [Re: Chilean6]
    #25268293 - 06/14/18 04:33 PM (5 years, 7 months ago)

Mason jars don't pour well, dribbles down the side. Wide openings too.


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Offlinerockpup
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Re: What do you use as agar bottle? [Re: bodhisatta]
    #25268374 - 06/14/18 05:10 PM (5 years, 7 months ago)

I am going to try this bottle? I think it should work well. My first try.


--------------------
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InvisiblebodhisattaMDiscordReddit
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Re: What do you use as agar bottle? [Re: rockpup]
    #25268376 - 06/14/18 05:11 PM (5 years, 7 months ago)

Yes, its made for pouring agar..


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InvisibleNighted
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Re: What do you use as agar bottle? [Re: rockpup]
    #25268382 - 06/14/18 05:14 PM (5 years, 7 months ago)

Quote:

rockpup said:
I am going to try this bottle? I think it should work well. My first try.





Oooh, that is a very nice jar. Different agar concoctions can vary in expansion when being sterilized. Be careful and don't fill more than a quarter of the way to be safe and avoid overflow.


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Edited by Nighted (06/14/18 05:14 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: What do you use as agar bottle? [Re: Nighted]
    #25268418 - 06/14/18 05:29 PM (5 years, 7 months ago)

a quarter of the way :justno:

you can fill that up to 450mL no problem.


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Invisibleelasticaltiger
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Re: What do you use as agar bottle? [Re: bodhisatta]
    #25268453 - 06/14/18 05:50 PM (5 years, 7 months ago)

Fungi perfecti recommended filling media flasks to the 50% mark.


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Offlinethe.raven
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Re: What do you use as agar bottle? [Re: rockpup]
    #25268461 - 06/14/18 05:57 PM (5 years, 7 months ago)

Quote:

rockpup said:
I am going to try this bottle? I think it should work well. My first try.





Get this. A borosilicate media bottle, they're are made for pouring agar. The rim is designed to reduce drips, the glass is strong, and the cap (GL45) is designed for autoclaving. Several other GL45 caps can be purchased for specific applications (thermocouples, etc). At $10-20 (US), they are less fun than drinking using a bottle of wine but the cost is worth the convenience.

I have two and I am considering more. I like to prepare agar (mix and PC) ahead of time, refrigerate, and reheat when needed. I typically make 500mL (10g Agar, 7.5g malt extract, 1g yeast if at all, I haven't noticed a difference in my cultures with/without using yeast), fill the PC to match the level of the agar, slightly loosen the foil wrapped caps, and cycle on at 15psi for 20-25min. If I go longer my agar usually darkens, carmelizes. 500mL is good for 20 plates, 25-28 if I'm really efficient with my pours.


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Offlinerockpup
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Re: What do you use as agar bottle? [Re: the.raven]
    #25268487 - 06/14/18 06:12 PM (5 years, 7 months ago)

Yeah kinda excited but nervous to do my first pour-I have agar down no problem's there-It grows lot's of stuff haha-just my fucking no pour containers are useless. Mold creeps in under the seal.I bought sterile plates just waiting on some parafilm. I hate not being able to sterilize myself-I am buying glass petrie dish's-there wort it.Don't know if they work better but I can sterilize them myself.


--------------------
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RIP 1989-2001-The best of time's
I miss molly.


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