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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 4 months
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PE6 ms sectoring on agar
#23752275 - 10/19/16 03:05 PM (7 years, 4 months ago) |
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Here are two different plates and three pics.

The following two are the same:


These were inoculated with a ms swab and I haven't transfered yet. I'm relatively new to agar and haven't seen these divisions on each plate before unless mold was growing, but I don't see any mold either.
Usually when I noc from ms I get thick solid growth with no distintions, yet here I see them clearly.
Is the sectoring something I should be worried about, or does anyone know what's going on? I know they aren't different mono's, that would be impossible, but like I said I don't see a contam.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: PE6 ms sectoring on agar [Re: Kenetic]
#23752335 - 10/19/16 03:27 PM (7 years, 4 months ago) |
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Looks kinda like mold mycelium to me 
I can look again on a bigger screen later, but i think your cultures are mold
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Re: PE6 ms sectoring on agar [Re: dankington]
#23752344 - 10/19/16 03:32 PM (7 years, 4 months ago) |
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I also believe they are mold. Does not look correct.
Possibly a contaminated swab? Not sure.
I'd wait on a Tc to get in here.
Shouldn't be long.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 4 months
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Re: PE6 ms sectoring on agar [Re: morty422]
#23752527 - 10/19/16 04:45 PM (7 years, 4 months ago) |
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I think I see a bacterial colony in the fist pic. Looks like the myc is sectoring around it. It's been growing for about a week now and it isn't growing super fast like mold does though? I did all ten plates with no gloves this time and noticed some bacteria on a few other plates today. I usually don't get contams very often but I was being lazy.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Re: PE6 ms sectoring on agar [Re: Kenetic]
#23752693 - 10/19/16 05:40 PM (7 years, 4 months ago) |
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Quote:
kenetic said: I think I see a bacterial colony in the fist pic. Looks like the myc is sectoring around it. It's been growing for about a week now and it isn't growing super fast like mold does though? I did all ten plates with no gloves this time and noticed some bacteria on a few other plates today. I usually don't get contams very often but I was being lazy.
If you have the time, attempt to clean it up by transferring some of the spots you feel are mycelium, it really can't hurt unless you simply don't have the time or plates available.
Unless anything is sporulating, it won't hurt anything...
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