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OfflineDARKINTENT
Stranger
Registered: 09/21/16
Posts: 2
Last seen: 1 year, 1 day
no growth 3 weeks!!
    #23750375 - 10/18/16 09:26 PM (7 years, 3 months ago)

Hello shroomery I'll start by saying this is my first post on this account and it is my first time trying grain. Having said that I'll start with my basic setup for this experiment.I got my spores from a site sanctioned company same one I used several years ago when I first came to the shroomery and had a few successful pf TEK grows! After a lot of reading I decided to go with popcorn.I'm aware of the negatives it was convenient and I've accepted the pros and the cons. I have 6 pint wide mouth jars with my own self healing innoc port.I've proven there effectiveness previously. The corn slow simmered for around 3 hrs until I could easily squash them. They were well hydrated and around 10% burst I then rinsed excessively well loaded the jars and PCd @12 lbs for 90 minutes.I opted to pull them out while hot and closed the lids tight. I later released the resulting vac via    Iso alcohol soaked cotton balls stuffed in a syringe. Inoculation occurred in glove box and was well executed. They were left in a cabinet and picked up and once a day examined and put down. That's it no contamination no mycelium 3 weeks later! Anyone experienced this before.give me some ideas as to what's responsible. Also pcd several jars at the same time as those that were inoculated.I cracked one open yesterday was thinking maybe bacteria but it smelled fresh so I don't think it's contamination. Anyways thank in advance.                DI


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Invisiblefreespeech
disciple
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Registered: 12/12/08
Posts: 1,745
Loc: PNW
Re: no growth 3 weeks!! [Re: DARKINTENT]
    #23750394 - 10/18/16 09:31 PM (7 years, 3 months ago)

All you can do is wait. If you want advice, mine would be to do a more standard grain attempt using rye and filter discs instead of popcorn and ports.


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Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 13 minutes
Re: no growth 3 weeks!! [Re: DARKINTENT]
    #23750398 - 10/18/16 09:34 PM (7 years, 3 months ago)

You shot ms to popcorn?  It should be trying soon. The one you opened and sniffed is likely toast. 10-15%chance your jars turn white. 

Check out agar for better rocking grains in the future.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Offlinetump
ban the undead
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Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
Re: no growth 3 weeks!! [Re: DARKINTENT]
    #23750414 - 10/18/16 09:39 PM (7 years, 3 months ago)

First welcome back. Some times spores take a while to germinate on grains. Up two 6 weeks. They don't like popcorn in my experience. Sometimes bacteria infections dont smell. Id also recommend pcing longer then 90 mins if it only gets to 12psi.
Any good tc will tell you to test with agar. So pasty plates are a good plaxe to start.


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OfflineDARKINTENT
Stranger
Registered: 09/21/16
Posts: 2
Last seen: 1 year, 1 day
Re: no growth 3 weeks!! [Re: tump]
    #23750451 - 10/18/16 09:51 PM (7 years, 3 months ago)

Yeah I forgot to mention I tried agar for the first time as well all contams my agar game needs some changes but I was holding hope for the grain. 6 weeks for ms direct if I'd of known that I would have just ms'd a half pint jar pf style and transferred to the corn. So it could really stillgerm after this long? And by the way the jar I sniffed will contaminate there was no precaution taken it wasnt inoculated I left it as a control and I still have another!


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Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 13 minutes
Re: no growth 3 weeks!! [Re: tump]
    #23750485 - 10/18/16 09:59 PM (7 years, 3 months ago)

Not much of a difference between 12 and 15... I think 3-4 degrees.

Nothing to test tump.

Keep pops on standby... In the meantime...

Start over with agar and a sab. UTSF

Set jars on a shelf where you can see them and don't pick them up until you see white or green.

If you shook it after you shot it, it will take much longer for the spores to find their mates.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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