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Offlinemr_dude
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Re: Agar, grains, h-poo [Re: Boogieman47]
    #23700981 - 10/02/16 05:13 PM (7 years, 4 months ago)

Hey dudes,
pics from the second transfer. looks good? they look kinda small to me (these are the small petri dishes 60 mm in diameter)


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Offlinemr_dude
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Re: First Attempt at Agar and Grain [Re: mr_dude]
    #23713384 - 10/06/16 02:33 PM (7 years, 4 months ago)

Updated pictures of the second transfer. My popcorn grains are cooling inside the PC right now and im going to use wedges from these dishes to make 6 or 7 master grain jars. I will however do a third transfer to new dishes at the same time and see how it goes coz this is some interesting stuff :eek:


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InvisibleMycolorado
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Re: Agar, grains, h-poo [Re: mr_dude]
    #23713559 - 10/06/16 03:29 PM (7 years, 4 months ago)

Quote:

mr_dude said:
Im not pursuing this just rather curious. first i thought these plates (result of first transfer) were infested with cobweb since they appeared to be growing in 3d rather than 2d. they appeared to be bulging from the agar and not leveled. but now im not sure, do they look alright to u?







These look good.  What's your agar recipe...what's the nutrient %?  Hard to tell but your colonies look very tomentose and bulging as opposed to rhizo or linear which isn't a bad thing, could be due to the nutrient content or could just be the way it grows...Just curious as I'm running a comparison of 2% and 4% MEA to look at the effects on mycelium growth.  Maybe hold the plates up to a light to get better detail for the pic.  Here's a 4% plate doing very well.


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Offlinemr_dude
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Re: First Attempt at Agar and Grain [Re: Mycolorado]
    #23713627 - 10/06/16 03:50 PM (7 years, 4 months ago)

They do look kind of bulgy dont they? Also, they are sucking up all the nutrients since Im noticing the color of the agar in the plate is starting to become lighter (even clear almost) as opposed to the regular agar color in the dishes that are not yet inoculated. What gives?
My agar recipe:
5_g instant potato flakes
7_g dextrose
9_g agar
500_ml water

Took a few pictures while holding a plate up to a light, check it out


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Offlinewtfcrazymofo
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Re: Agar, grains, h-poo [Re: mr_dude]
    #23713661 - 10/06/16 04:02 PM (7 years, 4 months ago)

Good. :thumbup:


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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InvisibleMycolorado
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Re: Agar, grains, h-poo [Re: wtfcrazymofo]
    #23714411 - 10/06/16 08:58 PM (7 years, 4 months ago)

Could just be the PDA...typically, there's more of a rhizo pattern on the lower nutrient agar...your recipe is 2.4%.  Would be cool to plate some up on DFA or MEA to see if grows differently.  They look pretty fucking good and isolated down, but that could very well be a result of the agar...Be nice to see some even flushes off those.


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Offlinemr_dude
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Re: Agar, grains, h-poo [Re: Mycolorado]
    #23715289 - 10/07/16 06:46 AM (7 years, 4 months ago)

Mycolorado, u peaked my curiosity so ill work up a 3% MEA batch (15 malt, 10 agar, 500ml) and use it for further transfers. ill also do my best to fruit using myc i currently have and compare with the one i'd hopefully get from the upcoming transfer. wish me luck!

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Offlinemr_dude
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Re: First Attempt at Agar and Grain [Re: mr_dude]
    #23749478 - 10/18/16 04:44 PM (7 years, 4 months ago)

Time to do some math my friends :frown:
Ive gotten some jars, trays, and 2 tubs.
From what i could dig up here, people tend to just eyeball it. but I've never done any spawn/bulk and tubs before and am having a hard time crunching my numbers. (The quarts and pints used here are US liquid; I also did some rounding up on a few calculations):

JAR

570 ml --> 0.6 Quarts --> 1.2 Pint

TRAY
Width: 22.5 cm --> 9 Inch
Length: 33 cm --> 13 Inch
Height: 8 cm --> 3 Inch
6 Liter --> 6.3 Quarts

TUB
Width: 41 cm --> 16 Inch
Length: 54 cm --> 21 Inch
Height: 35 cm --> 14 Inch
77.5 Liter --> 80 Quarts

I'm planning on ending up with 30 of these jars fully colonized before moving to bulk. Assuming that was doable and turned out right, how should i proceed?
- Should I mix in trays and wait for colonization or should I mix in a monotub right away?
- If using trays, do I just put them in a SGFC when colonized or pour them all together in a monotub instead?
- If using manure, do I just keep mixing with for instance 2:1 ratio of manure/spawn until its 4-5 inches? or what is the best ratio if im not cheap on spawn? 1:1?

Any guidance is greatly appreicated  :bigkiss:

On a side note, this is a sample of the dishes I ended up with. This one had a little sectoring, so i sacrificed it for a decent picture seeing that i have quite a few that look the same. is it good?


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OfflineTh3Issu3
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Re: First Attempt at Agar and Grain [Re: mr_dude]
    #23749517 - 10/18/16 04:59 PM (7 years, 4 months ago)

you need to go buy some green food coloring and put a few drops in when you are making your agar, so you can see things better

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Offlinemr_dude
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Re: First Attempt at Agar and Grain [Re: Th3Issu3]
    #23749525 - 10/18/16 05:02 PM (7 years, 4 months ago)

Yup already got food coloring for my next batch. Arent the pictures clear tho? Something wrong with em?
Other than that, any thoughts?

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Invisiblemorty422
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Re: First Attempt at Agar and Grain [Re: Th3Issu3]
    #23749591 - 10/18/16 05:26 PM (7 years, 4 months ago)

Quote:

Th3Issu3 said:
you need to go buy some green food coloring and put a few drops in when you are making your agar, so you can see things better




Why green? Why not purple? Or red?


Color in the agar might make things slightly easier to see -but it's not that big of a deal.

Op.

10 of your 1.2 pint jars will equal around 6 quarts for your 80 quart tub.

My favorite amount of spawn to use for a 66qt tub is 5qts spawn. 6quarts to your 80qt tub is the same in relation.

I would worry less about an ideal spawn-sub ratio and more about good conditions and clean grain spawn.

I would use ten of your jars per tub and simply create a 3-4" deep substrate evenly mixed with your spawn.

I would not use trays unless you are attempting to test out multiple sets of cultures.

I would also use coir as it is extremely user friendly -it can be pasteurized, sterilized, whatever and cubensis mushrooms love it. In my opinion, it is just as, if not more nutritious than manure.
Bucket-twk is too easy and is a tested method with awesome results.
If you haven't seen it yet it is in my signature.

I would simply line your tub with a liner and pour away. No exact science.

Strive for a yield of 1 dry oz per quart of spawn used in your tub-first flush.

If you go by my above-strive for 6oz dry first flush as a starting point. The more you learn how to dial-in your tubs and the more you continue to work on good genetics and clean spawn through agar- the more this number will increase.

Using 6quarts in your tub is a good starting point... Not too many eggs in one basket and you should see a nice quick spawn run.

:cheers:

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Offlinemr_dude
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Re: First Attempt at Agar and Grain [Re: morty422]
    #23749647 - 10/18/16 05:47 PM (7 years, 4 months ago)

Yes I have come across the bucket tek and its among my bookmarks. Thanks a bunch your answer is just what I needed.
The reason I chose manure over choir is because in my circumstances, it is a lot easier to acquire and it does not seem too hard to handle. But if you say you believe choir is even better and that does seem like the popular opinion, ill give it a go. by your calculations, i can easily do 2 tubs at once. do u advise against it? im just thinking in the sense of go big or go home..

cheers!

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Invisibleazur
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Re: First Attempt at Agar and Grain [Re: mr_dude]
    #23749667 - 10/18/16 05:56 PM (7 years, 4 months ago)

Like, dude man, uh...like, this thread totally reads like cheech and Chong man


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Invisiblemorty422
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Re: First Attempt at Agar and Grain [Re: mr_dude]
    #23749807 - 10/18/16 06:44 PM (7 years, 4 months ago)

Quote:

mr_dude said:
Yes I have come across the bucket tek and its among my bookmarks. Thanks a bunch your answer is just what I needed.
The reason I chose manure over choir is because in my circumstances, it is a lot easier to acquire and it does not seem too hard to handle. But if you say you believe choir is even better and that does seem like the popular opinion, ill give it a go. by your calculations, i can easily do 2 tubs at once. do u advise against it? im just thinking in the sense of go big or go home..

cheers!




If you have 50 jars do 5 tubs at once... hell, I have 8 rolling now.

Albeit-this is the most I've had going at once-I usually have 4-6.

If you want to make a systematic process -now's the time to start it.

If you simply want some weight-do them all.

No science there either. :shrug:

:cheers:

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Offlinemr_dude
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Re: First Attempt at Agar and Grain [Re: morty422]
    #23782184 - 10/29/16 09:33 AM (7 years, 4 months ago)

This is what my jars look like atm
should i have used smaller agar wedges? as u may have noticed, the are big chunks, will that be an issue? i think not.


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Invisiblemorty422
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Re: First Attempt at Agar and Grain [Re: mr_dude]
    #23782564 - 10/29/16 12:46 PM (7 years, 4 months ago)

I like to add a wedge-let it recover-shake the wedge to create multiple points of growth and then wait until the jar is about 40-50% colonized.
After the initial shake and the grains are 40-50%, I do one more shake to really bring them to completion.

This process usually takes 12-14 days for me with qt jars.

In the past, I have attempted to allow the wedge to grow out without the first shake. It always seemed to take much longer as I believe the mycelium didn't want to leave the wedge piece without first exhausting the first form of media.
By shaking the wedge-I feel like I am sort of getting the mycelium off the wedge and onto the grains where I want it - and the more rapid growth seems to justify my opinion.

Wedge size being large is a better thing in my opinion, and if you are going to shake a large wedge- it is almost the same as shaking smaller wedges that add up to the same size.

Your choice of grain is a strange one...popcorn is the worst grain I can think of to use in mushroom cultivation... it is expensive, prone to contamination and provides less yields (imo) than other more cost effective grains.

Oats have been my thing lately-they break up super easy, are nutritious as fuck, and have provided me with better yields with my current cultures.

I grabbed 271 dry grams off my last monotub with 5 qts spawn and bucket tek-first flush. Oats are the tits! I highly recommend them.

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Offlinemr_dude
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Re: First Attempt at Agar and Grain [Re: morty422]
    #23782828 - 10/29/16 02:22 PM (7 years, 4 months ago)

Actually, popcorn is RR's grain of choice :crazy:
I followed this tek and the convenient thing about it is the prepping time. pretty quick and easy. I have yet to find out about contams and yield tho. plus popcorn here is quite cheap :eek:

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Invisibledankington
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Re: First Attempt at Agar and Grain [Re: mr_dude]
    #23783319 - 10/29/16 05:42 PM (7 years, 4 months ago)

If popcorn were RR's grain of choice, he'd have made a tek about it instead of rye berries…

I hate popcorn myself. I know some people have had success with it, but it's crazy expensive, and can be quite problematic for a few reasons.

It's probably the most expensive grain you could buy for spawn.

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Invisibleazur
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Re: First Attempt at Agar and Grain [Re: dankington]
    #23783359 - 10/29/16 05:58 PM (7 years, 4 months ago)

I've never tried it.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisiblemorty422
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Re: First Attempt at Agar and Grain [Re: azur]
    #23783846 - 10/29/16 08:59 PM (7 years, 4 months ago)

Quote:

azur said:
I've never tried it.




Don't.

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