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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: maynardjameskeenan]
#23765726 - 10/24/16 12:08 AM (7 years, 3 months ago) |
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I'm sure your shrink told you it's more complex than that.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: DavidReishi] 3
#23765790 - 10/24/16 12:51 AM (7 years, 3 months ago) |
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No worries kiddo.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Hunter hunter
See er


Registered: 04/02/14
Posts: 2,845
Loc: Pickin yer patch
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Lol stop trying to troll Maynard. He is chill as fuck.
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Eat the meat that’s at your feet.
Edited by Hunter hunter (10/24/16 12:57 AM)
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Alan Rockefeller] 1
#23765806 - 10/24/16 01:08 AM (7 years, 3 months ago) |
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Quote:
Alan Rockefeller said:
Quote:
stevo said: Shit. Do you have a link that shows this? I have planted all 4 of these in one bigass chip dump. This is going to be confusing if/when they fruit, amirite?
It's unpublished work by the European guy who we sent some prints to a couple years back. Not sure if it will get published, I just have a very rough draft. I can send it to you if you like.
Your wood chip patch might get interesting, you'll want to take photos of them each day as they develop. Sometimes they are hard to tell apart from one photo, but seeing the whole life cycle should make it easy. Time lapse would be pretty cool. The four species can be told apart by sequencing the DNA of the ITS gene. Psilocybe subaeruginosa and P. azurescens are the closest, with only 1 base pair difference. That small difference is stable across multiple collections though, and is enough to tell them apart.
Here is a BLAST on the Psilocybe allenii holotype:

And here is how it compares to the holotype of Psilocybe cyanescens:

Psilocybe azurescens

Psilocybe subaeruginosa

And Psilocybe weraroa

It's all very interesting Alan and sorry David for further derailing your thread which is also interesting and somewhat amusing, nice finds by the way.
I'm interested to learn if it's possible to artificially create this...

By mating isolated monokaryons of this...

With isolated monokaryons of this...

In macroscopic form there are many different forms of the first partially secotioid mushroom I posted above and I wonder if they are the result of natural hybridisation of Psilocybe subaeruginosa and P. weraroa.
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N05482
cyantist



Registered: 10/08/14
Posts: 703
Loc: riparian zoneaparte
Last seen: 3 months, 15 days
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: DavidReishi]
#23765825 - 10/24/16 01:32 AM (7 years, 3 months ago) |
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this is the best ride in the park today that's for sure! Imma ride it all day tomorrow if it's still open!
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Have a nice day! psilocybe cyanescens time lapse
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AllDay420
Ghost0420

Registered: 09/03/16
Posts: 301
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: N05482]
#23765834 - 10/24/16 01:41 AM (7 years, 3 months ago) |
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Yay, it's inski! I love your work dude, I honestly do! You are my favorite user, mad respect for you brother!
inski, over humble yet over knowledgeable, it's a win win scenario.
I saw inski as the last post and was like..........

inski, you would never be able to de-rail shit brother! If anything you have put the train back on the rails and gave that motherfucker a nitrous oxide engine!!!!!

Can you make P. subsecotioides by crossing isolated monokaryons of P. subaeruginosa and P. weraroa? Only one way to find out, am I right! 
Now, what's a monokaryon?
-------------------- Approved puppet.
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AllDay420
Ghost0420

Registered: 09/03/16
Posts: 301
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: AllDay420]
#23765847 - 10/24/16 01:55 AM (7 years, 3 months ago) |
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If you don't mind inski, I wonder, how would one go about isolating monokaryons from two species and mating them?
-------------------- Approved puppet.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: AllDay420]
#23765852 - 10/24/16 02:02 AM (7 years, 3 months ago) |
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Thanks
A monokaryon is the resulting mycelium of a single spore that has germinated, it is unable to produce fruitbodies until it 'mates' with another compatible monokaryotic mycelium which in turn creates a dikaryotic culture which is able to produce fruitbodies, basically two compatible spores must germinate and mate to produce the dikaryotic mycelium which is capable of fruiting.
The best and most accessible way of isolating monokaryons is by using the dilution to extinction method, a series of dilutions of a spore solution is made, the final dilution is used to inoculate an agar plate and as soon as any cultures are noticed they are transferred to new plates, to be sure the cultures are monokaryotic a small sample of the culture is examined with the light microscope, if there are no clamp connections present it is monokaryotic.
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AstaCrazyBull
Tatored



Registered: 10/14/10
Posts: 780
Loc: What's it Tahuya?
Last seen: 3 months, 4 days
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: inski]
#23765860 - 10/24/16 02:18 AM (7 years, 3 months ago) |
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Inski. Wow. Nice. That's fkn deep there. I really enjoyed that big time. I'm speaking for most. I have SO much to learn here. I see DR does too. Hey let's do this!
-------------------- The Great Spirit gave me permission to use all things that bear seed for my use. I personally choose non-toxic organic compounds and a couple fermentables.
 Cyan time lapse youtube link. https://youtube.com/watch?v=gtgr2SGHxog
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AllDay420
Ghost0420

Registered: 09/03/16
Posts: 301
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: inski]
#23765861 - 10/24/16 02:18 AM (7 years, 3 months ago) |
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No, thank you inski!

And thank you for your reply, you are a great teacher.
You did the dilution to extinction method to cultivate P. aucklandiae on your second attempt, but not on your first attempt, am I correct in stating that?
-------------------- Approved puppet.
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AllDay420
Ghost0420

Registered: 09/03/16
Posts: 301
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: AllDay420]
#23765864 - 10/24/16 02:20 AM (7 years, 3 months ago) |
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That makes two of us AstaCrazyBull
You have a fan club inski!
Awaiting more knowledge from inski like........
-------------------- Approved puppet.
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: AllDay420]
#23766388 - 10/24/16 10:37 AM (7 years, 3 months ago) |
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I've spoken to a man, with a project he has started yet put on hold.
He attempts to cross pan cyans, with ps. Cyans. To create cyans that would fruit everywhere essentially lol. Pretty cool guy. I'd have to dig up the info on how he went about this.
Liquid media and a centrifuge if I remember right were utilized to attain the clamp connections if u will...
Apparently two cells can be placed into a "PEG" solution. Then centrifuged. Dies can be used to aid the process. U then view the cells to see if the "protoplasts" have fused. He made it sound simple enough lol.
Very interesting stuff.
I personally wonder if it's not hybridization but simply phenotypic expressions if u will. And something underlying is possibly preventing us from seeing that they're all related. Just my personal uneducated speculation
Edited by Raven44 (10/24/16 10:44 AM)
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,646
Loc: Norvegr
Last seen: 5 hours, 52 minutes
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Raven44]
#23766401 - 10/24/16 10:44 AM (7 years, 3 months ago) |
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Quote:
Raven44 said: I've spoken to a man, with a project he has started yet put on hold.
He attempts to cross pan cyans, with ps. Cyans.
I strongly doubt it is possible to cross Panaeolus and Psilocybe.
--------------------
★★★★★
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Anglerfish]
#23766403 - 10/24/16 10:45 AM (7 years, 3 months ago) |
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Quote:
Anglerfish said:
Quote:
Raven44 said: I've spoken to a man, with a project he has started yet put on hold.
He attempts to cross pan cyans, with ps. Cyans.
I strongly doubt it is possible to cross Panaeolus and Psilocybe.
I strongly disagree
The man is rather brilliant.
"somatic cell nuclear fusion still occurs under PEG conditions the same way plant protoplasts fuse."
This is why I insinuated man could have altered nature and caused this find. It's possible. And people would totally do that. There are people doing it now most likely. It may also already have been done.
Everyone here is capable of doing so. Read GGMM by Paul stamets. He explains spore dilution to extinction. Tradd cotter also might do so in his book.
Then start playing with mono cultures. It's a cake walk. Just lots of time I imagine and some equipment
Edited by Raven44 (10/24/16 10:54 AM)
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Raven44]
#23766468 - 10/24/16 11:16 AM (7 years, 3 months ago) |
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What is this species???
I'm interested to learn if it's possible to artificially create this...
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seaspearo
Stranger


Registered: 01/27/16
Posts: 19
Last seen: 6 years, 2 months
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Raven44]
#23766721 - 10/24/16 12:43 PM (7 years, 3 months ago) |
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Are these active? I went to some shopping center in east bay today. When I first saw them, I thought just normal little brown mushrooms. Just for curious, I picked couple of them, at beginning the cut is watery, transparent liquid, no blue, but when I go back to home, I noticed they showed a little blue and a lot of grey or black now.


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RiverDweller1



Registered: 03/05/12
Posts: 4,347
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Raven44]
#23766754 - 10/24/16 12:54 PM (7 years, 3 months ago) |
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Quote:
Raven44 said:
Quote:
Anglerfish said:
Quote:
Raven44 said: I've spoken to a man, with a project he has started yet put on hold.
He attempts to cross pan cyans, with ps. Cyans.
I strongly doubt it is possible to cross Panaeolus and Psilocybe.
I strongly disagree
The man is rather brilliant.
"somatic cell nuclear fusion still occurs under PEG conditions the same way plant protoplasts fuse."
This is why I insinuated man could have altered nature and caused this find. It's possible. And people would totally do that. There are people doing it now most likely. It may also already have been done.
Everyone here is capable of doing so. Read GGMM by Paul stamets. He explains spore dilution to extinction. Tradd cotter also might do so in his book.
Then start playing with mono cultures. It's a cake walk. Just lots of time I imagine and some equipment
Thanks for sharing this, it certainly adds to the conversation and is not something to nay-nay. Lot's of reading can be found on the topic and mating different species on agar is nothing newly in practice.
Tradd Cotter and Stamets have most definitely done so. Cotter's latest book is quite informative and includes such info.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: seaspearo]
#23766979 - 10/24/16 02:18 PM (7 years, 3 months ago) |
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Quote:
seaspearo said: Are these active? I went to some shopping center in east bay today. When I first saw them, I thought just normal little brown mushrooms. Just for curious, I picked couple of them, at beginning the cut is watery, transparent liquid, no blue, but when I go back to home, I noticed they showed a little blue and a lot of grey or black now.



The mushrooms in the first picture aren't the same as the ones in the other two.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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seaspearo
Stranger


Registered: 01/27/16
Posts: 19
Last seen: 6 years, 2 months
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: maynardjameskeenan]
#23767041 - 10/24/16 02:37 PM (7 years, 3 months ago) |
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Quote:
maynardjameskeenan said:
Quote:
seaspearo said: Are these active? I went to some shopping center in east bay today. When I first saw them, I thought just normal little brown mushrooms. Just for curious, I picked couple of them, at beginning the cut is watery, transparent liquid, no blue, but when I go back to home, I noticed they showed a little blue and a lot of grey or black now.



The mushrooms in the first picture aren't the same as the ones in the other two.
They are the same, after picked them, I put into a plastic bag, when I back to home, I noticed it showed some blue or black. From the first picture, they did not seems active, do you know what kind are they?
Thanks!
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RiverDweller1



Registered: 03/05/12
Posts: 4,347
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: seaspearo]
#23767055 - 10/24/16 02:43 PM (7 years, 3 months ago) |
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Spore print please.
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