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InvisibleSirtalis
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: DavidReishi] * 3
    #23764774 - 10/23/16 05:03 PM (7 years, 3 months ago)

This thread is hilarious.  Jokes on you DavidReishi.  Maybe some day you'll look back at this thread and realize how ridiculous and childish you sound.

I hope a mod closes this thread soon.


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OfflineBlazeyy
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Sirtalis]
    #23764786 - 10/23/16 05:08 PM (7 years, 3 months ago)

:tweak:


--------------------
I give you the choice of 2 pills.

With each containing one of the following:
Cyanide... Psilocin... Would you take the risk?
Didn't think so.

This is why Positive Identification prior to consumption is important.


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OfflineSmilingPolitely
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Blazeyy] * 3
    #23764792 - 10/23/16 05:09 PM (7 years, 3 months ago)

a braggart and jerk, what a combo..


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InvisibleDavidReishi
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: SmilingPolitely]
    #23764799 - 10/23/16 05:11 PM (7 years, 3 months ago)

Quote:

SmilingPolitely said:
a braggart and jerk, what a combo..




What's that? An advert for yourself?


--------------------
Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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InvisibleRaven44
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: DavidReishi]
    #23764801 - 10/23/16 05:13 PM (7 years, 3 months ago)

Quote:

DavidReishi said:
Quote:

Raven44 said:
Those fruits are too concentrated imo.

I'm w joust. That's some professional spore bombing imo.






Would a patch like this change your mind? Lol, I don't mean as a bribe. But look how concentrated it is. I found the patch two years ago and I really doubt it was put there by hand. Sometimes they're just like that here in the Bay area...though that example may be a bit extreme.





But here's virtually the whole extent of the patch of P. allenii (yesterday), that sits at the same general location as the Azure patch. The latter covers 400-600% more area than these allenii.


Quote:

I hope ur right tho David, I hope azzies have been naturally acclimated or possibly even geneticly altered by man to produce a species fruiting in temps loke u have witnessed.




Actually my only position so far is that I don't think the patch is connected to any cultivation or 'spawning.' If I've ever mentioned the word "natural" regarding this patch, I meant it in the normal way we talk about the spreading of spores, including by means of human activity. Let's not forget that there's even people like SkagitHunter, who comes into direct contact with Azzies and just so happens to make periodic trips to the Bay area.




Well, in reality, no one here can hold a firm stance on weather or not they r natural or if man had anything to do w it directly or indirectly..

Sooo, it's all purely speculation. Period end of story.

It also cannot be confirmed as the first case of azzies. It MAY be the first documented case tho.

So it's all largely insignificant.

What is significant is weather or not there r more fruiting in the area. Also, if they start to spread in that area.

I'd help em out to find out for myself via spore bombing and spreading mycelium fuck it who cares if man did it or nature it's bad ass either way


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InvisibleRaven44
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Raven44]
    #23764803 - 10/23/16 05:14 PM (7 years, 3 months ago)

Make me think of a picture of cyans and allenii fruiting next to each other

Appearing as the same species expressing itself in diff phenotypic expressions if u will

Hmmmm mmmm

Cyans, alleni, azzies????, all in the same spot. Maybe they r just one species expressing diff phenotypic expressions as they will.

I suuure wonder


Edited by Raven44 (10/23/16 05:16 PM)


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InvisibleAllDay420
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: DavidReishi] * 1
    #23764810 - 10/23/16 05:17 PM (7 years, 3 months ago)

Come on guys, please just be nice to David, just give him a break why don't yas?

And David, please just stop replying negatively to the negativity.
If you replied positively to the negativity this would not be such a shit show and it would be a positive learning environment.

Thank you David for contributing some interesting contend.
And thank you to everyone else who shared some great wisdom.


--------------------
Approved puppet.


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InvisibleAllDay420
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Raven44]
    #23764828 - 10/23/16 05:24 PM (7 years, 3 months ago)

Fuck the shit show man, let this thread live on in great discussion and positivity!!!

Quote:

Raven44 said:
Make me think of a picture of cyans and allenii fruiting next to each other

Appearing as the same species expressing itself in diff phenotypic expressions if u will

Hmmmm mmmm

Cyans, alleni, azzies????, all in the same spot. Maybe they r just one species expressing diff phenotypic expressions as they will.

I suuure wonder



Oh fuck yeah mate, that makes two of us, same goes with P. subaeruginosa.

Sometimes I wonder!
Subs, azurs, cyans and allenii.. Are they REALLY all different species..
OR ARE THEY JUST DIFFERENT PHENOTYPES OF THE SAME DAMN SPECIES?????

:lol:

Finding out that all these different species could actually be synonymous like P. tampanensis, P. mexicana and P. galindoi for example, apparently all synonymous.

And P. semilanceata and P. strictipes apparently synonymous.

Makes one wonder!

Your thoughts? I would like anyones input especially Alan's.


--------------------
Approved puppet.


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OfflineAlan RockefellerM
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: AllDay420]
    #23764865 - 10/23/16 05:41 PM (7 years, 3 months ago)

Quote:

AllDay420 said:

Sometimes I wonder!
Subs, azurs, cyans and allenii.. Are they REALLY all different species..
OR ARE THEY JUST DIFFERENT PHENOTYPES OF THE SAME DAMN SPECIES?????






They are very close, but have different geographical distribution, macroscopic characteristics, chemical characteristics and ITS DNA sequences.  The four can mate but it's not known if the offspring are fertile or what they would look like.

They are certainly not the same organism, as spore prints, when cultivated always make fruit that looks like the species they came from.

You can lump them if you want, but I think narrower species concepts are better in this group.


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OfflineHammer92
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Alan Rockefeller] * 1
    #23764874 - 10/23/16 05:48 PM (7 years, 3 months ago)

Such a nice mixture of horseshit and great information in this thread
Classic shroomery


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Invisiblestevo

Registered: 04/11/05
Posts: 5,100
Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area *DELETED* [Re: Alan Rockefeller]
    #23764897 - 10/23/16 05:58 PM (7 years, 3 months ago)

Post deleted by stevo

Reason for deletion: .


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OfflineAlan RockefellerM
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: stevo]
    #23764925 - 10/23/16 06:10 PM (7 years, 3 months ago)

Quote:

stevo said:
Shit.  Do you have a link that shows this?  I have planted all 4 of these in one bigass chip dump.  This is going to be confusing if/when they fruit, amirite?





It's unpublished work by the European guy who we sent some prints to a couple  years back.  Not sure if it will get published, I just have a very rough draft.  I can send it to you if you like.

Your wood chip patch might get interesting, you'll want to take photos of them each day as they develop.  Sometimes they are hard to tell apart from one photo, but seeing the whole life cycle should make it easy.  Time lapse would be pretty cool.  The four species can be told apart by sequencing the DNA of the ITS gene.  Psilocybe subaeruginosa and P. azurescens are the closest, with only 1 base pair difference.  That small difference is stable across multiple collections though, and is enough to tell them apart.

Here is a BLAST on the Psilocybe allenii holotype:



And here is how it compares to the holotype of Psilocybe cyanescens:



Psilocybe azurescens



Psilocybe subaeruginosa



And Psilocybe weraroa




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InvisibleRaven44
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Alan Rockefeller]
    #23765040 - 10/23/16 06:54 PM (7 years, 3 months ago)

I don't mean to propose that all three or four species should be defined as the same species..

But perhaps there is something unknown here on this topic.

I'm aware that they all have diff genetic sequences but it seems there is something to learn here possibly

If they are all one, in theory.. then the fact that they can be printed and when grown the same physical expression is observed.. this would possibly say something beyond me atm admittedly.

Cordyceps can be observed to have changed its genetic sequence in response to it'd environment. According to things I've read. is this correct???

Possibly they psilocybes can change they're genetic sequence in response to environmental conditions. Which causes varieties of physical expressions...??? Purely speculating Here in a somewhat uneducated manner as some can tell I'm sure lol..

Have people that have isolated their own strains from the wild ever thought to test genetic sequences the entire step of the way? I think that'd be quite interesting to see if u can get ur genetic sequence to change via lots of time exposed to one type of environmental conditions....


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InvisibleliloldmeFacebook
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: AllDay420] * 1
    #23765051 - 10/23/16 07:00 PM (7 years, 3 months ago)

I love spreading colonized woodchips in fucked up spots...


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InvisibleRaven44
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Raven44]
    #23765064 - 10/23/16 07:04 PM (7 years, 3 months ago)

I also think w the above post by alan that it is quite interesting that subs and azzies look very similar as well.. hmmm


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OfflineAlan RockefellerM
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: liloldme]
    #23765089 - 10/23/16 07:14 PM (7 years, 3 months ago)

Quote:

Raven44 said:
Cordyceps can be observed to have changed its genetic sequence in response to it'd environment. According to things I've read. is this correct???

Possibly they psilocybes can change they're genetic sequence in response to environmental conditions. Which causes varieties of physical expressions...??? Purely speculating Here in a somewhat uneducated manner as some can tell I'm sure lol..





We are looking at https://en.wikipedia.org/wiki/Internal_transcribed_spacer, so it's not the part of the DNA that does anything.  Sequencing this costs about $25.

Quote:

Have people that have isolated their own strains from the wild ever thought to test genetic sequences the entire step of the way? I think that'd be quite interesting to see if u can get ur genetic sequence to change via lots of time exposed to one type of environmental conditions....




You would need to sequence the whole genome to find changes like that, it costs about $2000 to do that - so an expensive project to track the DNA changes over generations.  Scientists do similar things all the time in their research, here is a recent example: http://news.wisc.edu/super-yeast-has-the-power-to-improve-economics-of-biofuels/




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InvisibleJoust
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: Alan Rockefeller]
    #23765168 - 10/23/16 07:44 PM (7 years, 3 months ago)

Quote:

Alan Rockefeller said:
Quote:

Raven44 said:
Cordyceps can be observed to have changed its genetic sequence in response to it'd environment. According to things I've read. is this correct???

Possibly they psilocybes can change they're genetic sequence in response to environmental conditions. Which causes varieties of physical expressions...??? Purely speculating Here in a somewhat uneducated manner as some can tell I'm sure lol..





We are looking at https://en.wikipedia.org/wiki/Internal_transcribed_spacer, so it's not the part of the DNA that does anything.  Sequencing this costs about $25.

Quote:

Have people that have isolated their own strains from the wild ever thought to test genetic sequences the entire step of the way? I think that'd be quite interesting to see if u can get ur genetic sequence to change via lots of time exposed to one type of environmental conditions....




You would need to sequence the whole genome to find changes like that, it costs about $2000 to do that - so an expensive project to track the DNA changes over generations.  Scientists do similar things all the time in their research, here is a recent example: http://news.wisc.edu/super-yeast-has-the-power-to-improve-economics-of-biofuels/






My professor just quoted me 8000 with full Genome sequencing..... but the problem is putting it together.... which requires mad server space? right?

At least this was my impression.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Invisiblemaynardjameskeenan
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: maynardjameskeenan] * 2
    #23765441 - 10/23/16 09:43 PM (7 years, 3 months ago)

Quote:

DavidReishi said:
Yes, Alan, I re-read the thread. I think it went south in the second and fourth post, when Maynardjameskeenen, without any words of encouragement for what might be the most amazing find ever in the Bay area, turned the topic of the thread into the insistence that the patch was man-made. It wasn't so much that his words didn't ring true according to my observations, for as can be seen in my first reply to him I simply stated as much, but rather that he set the tone of the thread to be one not of friendliness and mutual amazement, but of giving in to the jealousy in one's heart and trying to take away from the joy of the find. Which is precisely what proceeded.




-----------------

Quote:

DavidReishi said:
What do you think? I'm on location...more later!




to which I repsonded

Quote:

maynardjameskeenan said:
Sure looks like it to me. I would be willing to bet someone introduced them. What kind of habitat are they growing in?




I didn't mean to sour anyone's thread, I was trying to help you identify your mushrooms. Notice how I didn't respond or ague to your "Because mushrooms grow naturally."
People on this forum (especially TI's and MH&I in general) normally aren't dickheads, we come here to help people and look at cool mushrooms.
You asked what people thought and I was asking you some questions about them to make sure they were azurescens. Your own name calling and poor attitude ruined this thread- its classic 'victim playing'.

Your finds are really really cool, no doubt about that!
I hope that in the future your hubris and egotism don't overshadow such amazing finds. Right now you're the most prolific hunter in the bay area that posts on this site, keep it up slugger! :congrats:


--------------------
May you be filled with loving kindness.
May you be well.
May you be peaceful and at ease.
May you be happy.



AMU Q&A


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InvisibleDavidReishi
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: maynardjameskeenan]
    #23765708 - 10/23/16 11:59 PM (7 years, 3 months ago)

Are you sure you're not using someone else's "I swear I'm not jealous" template or something?


--------------------
Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Invisiblemaynardjameskeenan
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Re: The first case of Psilocybe Azurescens in the San Francisco Bay Area [Re: DavidReishi] * 3
    #23765719 - 10/24/16 12:02 AM (7 years, 3 months ago)

Right, no one in Oregon or Washington can find azurescens. Not like you can champ.


--------------------
May you be filled with loving kindness.
May you be well.
May you be peaceful and at ease.
May you be happy.



AMU Q&A


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