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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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ELI5: How does Agar help reduce contam rate?
#23748160 - 10/18/16 08:03 AM (7 years, 3 months ago) |
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Whenever anybody here posts a photo of their bacterial/moldy/otherwise contaminated cake or grain spawn or what have you, there's always at least one guy who says: "LEARN AGAR."
How could learning Agar decrease contam rate? If anything, in my mind, the complexity and difficulty and all the steps required would only increase your chances of running into any contaminants. Additionally, you still have to get the agar into your spawn bag/however that works.
Can somebody show me exactly what the agar procedure is, but more importantly, how is it beneficial (in the context of contams, I understand how it would be beneficial for selecting favorable genes).
Thanks!
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: ELI5: How does Agar help reduce contam rate? [Re: Plazmotech]
#23748171 - 10/18/16 08:13 AM (7 years, 3 months ago) |
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The very basic question you have can be answered by watching the lets grow mushrooms series. YouTube it and watch the agar section.
Pour agar is not hard, the only reason you probably said that is because you never tried.
The last line of your post answered your own question. Agar is for selecting genetics and eliminating contams, I promise it's not more complex than that.
Heres a pictorial but watch the video series too. https://www.shroomery.org/forums/showflat.php/Number/18430998
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
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Re: ELI5: How does Agar help reduce contam rate? [Re: Plazmotech]
#23748180 - 10/18/16 08:17 AM (7 years, 3 months ago) |
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Quote:
Plazmotech said: Whenever anybody here posts a photo of their bacterial/moldy/otherwise contaminated cake or grain spawn or what have you, there's always at least one guy who says: "LEARN AGAR."
How could learning Agar decrease contam rate? If anything, in my mind, the complexity and difficulty and all the steps required would only increase your chances of running into any contaminants. Additionally, you still have to get the agar into your spawn bag/however that works.
Can somebody show me exactly what the agar procedure is, but more importantly, how is it beneficial (in the context of contams, I understand how it would be beneficial for selecting favorable genes).
Thanks!
It's not as hard as you think it to be. When you get a print or spore syringe, it will always be less than 100% clean. By starting on agar, you "clean up" your mycelium. By clean up I mean you can view the grow and section off healthy clean mycelium from contaminations and transfer them onto new agar. Repeating the process will only help insure your grow is clean and healthy. All this is done in front of a flow hood or in a still air box.
Munchauzen has a couple of videos up to show the process. Search his name in the search bar.
You worry about a clean grow first, then worry about gene selection. You clone or take a tissue sample to agar from a healthy fruit and start the transfer process over again until you get what you want.
-------------------- I would like to acquire anything I don’t have
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
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Re: ELI5: How does Agar help reduce contam rate? [Re: Plazmotech]
#23748184 - 10/18/16 08:18 AM (7 years, 3 months ago) |
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As I wrote in your other thread a spore print is not sterile and can contain 'hidden' mold spores or bacteria.
If you transfer spores to agar you can see if it is healthy mycelium or not . It is also possible to separate healthy sectors from contaminated sectors on one dish.
If you inoculate a jar with spores you never now if there are contams or not until you see them. You wasted time and grain.
That's a very basic description.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: ELI5: How does Agar help reduce contam rate? [Re: enlightenment]
#23748208 - 10/18/16 08:28 AM (7 years, 3 months ago) |
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: ELI5: How does Agar help reduce contam rate? [Re: Josex]
#23748257 - 10/18/16 08:48 AM (7 years, 3 months ago) |
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Thanks guys! Do you think it would be effective to do agar in a basic glovebox?
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
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Re: ELI5: How does Agar help reduce contam rate? [Re: Plazmotech]
#23748267 - 10/18/16 08:51 AM (7 years, 3 months ago) |
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Pull off the gloves then your in business (sab in other words)
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: ELI5: How does Agar help reduce contam rate? [Re: Plazmotech]
#23748268 - 10/18/16 08:51 AM (7 years, 3 months ago) |
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Quote:
Plazmotech said: Do you think it would be effective to do agar in a basic glovebox?
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: ELI5: How does Agar help reduce contam rate? [Re: mrmazdarx9]
#23748649 - 10/18/16 11:24 AM (7 years, 3 months ago) |
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Why pull them off? Wouldn't it be less sterile that way?
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
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Re: ELI5: How does Agar help reduce contam rate? [Re: Plazmotech]
#23748656 - 10/18/16 11:29 AM (7 years, 3 months ago) |
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No, we all use still air boxes now if not flow hoods. Gloveboxes basically make the job a pain in almost every way for very little to no extra protection from contamination. I constructed one before reading up on here, something I had stupidly balanced inside the box slipped while I was wearing the gloves, and as a knee jerk reaction I tried to grab it as it fell throwing the whole box and its contents across the room and the gloves ripped off
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
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Re: ELI5: How does Agar help reduce contam rate? [Re: Plazmotech]
#23748659 - 10/18/16 11:31 AM (7 years, 3 months ago) |
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Every time you move your gloves around in a glovebox they swoosh all the dirty air around inside and can even draw air in from the outside of the box, SABs don't do this nearly as badly. Still air boxes (SABs, glove boxes w/o gloves) are the current standard unless you have a flowhood.
This post explains it best: https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: ELI5: How does Agar help reduce contam rate? [Re: Plazmotech]
#23748672 - 10/18/16 11:34 AM (7 years, 3 months ago) |
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Quote:
Josex said: Bonus SAB
You can find that answer in the links I posted and pretty much everything you need to know, the whys and hows... everything. Now you have to decide if you want to put in some work and read them, or keep asking questions so that people do the work for you :spoonfeeding:
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
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Re: ELI5: How does Agar help reduce contam rate? [Re: Josex]
#23748677 - 10/18/16 11:34 AM (7 years, 3 months ago) |
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: ELI5: How does Agar help reduce contam rate? [Re: Greg]
#23748693 - 10/18/16 11:41 AM (7 years, 3 months ago) |
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Quote:
Greg said:


omg and I was thinking, why the heck isn't there a "spoodfeed" emoticon?? You made my day dude
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: ELI5: How does Agar help reduce contam rate? [Re: Josex]
#23748723 - 10/18/16 11:52 AM (7 years, 3 months ago) |
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Agar is easy but you're going to have a really difficult time with agar work if you dont have the tenacity to click some links and get the questions that shouldn't even be asked out of the way
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: ELI5: How does Agar help reduce contam rate? [Re: Josex]
#23750716 - 10/18/16 11:07 PM (7 years, 3 months ago) |
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Quote:
Josex said:
Quote:
Josex said: Bonus SAB
You can find that answer in the links I posted and pretty much everything you need to know, the whys and hows... everything. Now you have to decide if you want to put in some work and read them, or keep asking questions so that people do the work for you :spoonfeeding:
Sorry, I was on mobile when posting this question, wasn't in a position to be browsing the net & reading a bunch of articles. I have looked at your links though, thanks for the great resources! Thanks to you I've decided to invest into some agar plates and a SAB.
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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