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mrmazdarx9
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Registered: 05/15/16
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Some will fall out at first then it wedges in place. I get a bit come out when I rehydrate part way through after a few flushes
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AgarStudent
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Re: Shotgun Terrarium Tek [Re: mrmazdarx9]
#23749639 - 10/18/16 05:44 PM (7 years, 3 months ago) |
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Okay, Cheers
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AgarStudent
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I haven't invested in the perlite, yet, as a bag of course grain costs $40 from the hydro store. I thought I would try it with towels first to see if the concept worked before shelling out for perlite. Sorry to sound stingy, but it is an expensive hobby!
Okay - I have the AC going and it is set to 22 Deg C (72F) I took the cake out of the monotub that I had in before and tried to tie it up a bit so that the grains underneath were not exposed to fallout. (the top part is cased in peat/calcium carbonate). It has been colonising with daylight conditions for 15 days now.
I have misted it a couple of times - It does not seem like my tub is very humid - not a droplet of water on the walls. Hmm no pins or humidity?
Am I doing something wrong? Are the holes drilled right? Check it out - much appreciated.
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PortabellaFella 1
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Registered: 08/08/16
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Is that tray elevated off the paper towel?
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AgarStudent
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Registered: 01/25/15
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No not off the towel, but it is separated with the plastic that came out of the monotub. Do these strains do a lot better in poo and colonising in spawn bags? Looking at a tek by Bw86:
https://www.shroomery.org/forums/showflat.php/Number/10925421/fpart/1/vc/1
The tek that I am using here is from the truffle tek by violet:
https://www.shroomery.org/forums/showflat.php/Number/17910649/fpart/all/vc/1
Cheers
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bodhisatta 
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Registered: 04/30/13
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Loc: Milky way
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If you're growing sclerotia species follow stonesun's write-ups. The make everyone else's obsolete
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AgarStudent
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Re: Shotgun Terrarium Tek [Re: bodhisatta]
#23751460 - 10/19/16 09:22 AM (7 years, 3 months ago) |
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Oh yeah, nice guide by Stonesun and he has covered a lot of important details.
https://www.shroomery.org/forums/showflat.php/Number/13431948/fpart/all/vc/1
Do you know if you will get a better yield with the 'Grains spawned to bulk substrate' (with horse poo) over fruiting the 'Cased grain substrate' - The latter with no horse poo and just a casing layer?
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bodhisatta 
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Registered: 04/30/13
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You'll get a better yield spawning to bulk over casing grains pretty much universally with Psilocybe species that grow indoors
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Greenskybluegrass7
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Registered: 08/18/12
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Quote:
AgarStudent said: I haven't invested in the perlite, yet, as a bag of course grain costs $40 from the hydro store. I thought I would try it with towels first to see if the concept worked before shelling out for perlite. Sorry to sound stingy, but it is an expensive hobby!
Try a garden supply or hardware store they have it for under ten bucks a bag
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ComebackKid
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Registered: 05/27/16
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Quote:
Greenskybluegrass7 said:
Quote:
AgarStudent said: I haven't invested in the perlite, yet, as a bag of course grain costs $40 from the hydro store. I thought I would try it with towels first to see if the concept worked before shelling out for perlite. Sorry to sound stingy, but it is an expensive hobby!
Try a garden supply or hardware store they have it for under ten bucks a bag
Ftfy
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Substrate surface conditions / Monotub prep and care
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Juiceh
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Registered: 09/25/12
Posts: 3,208
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Quote:
AgarStudent said: I haven't invested in the perlite, yet, as a bag of course grain costs $40 from the hydro store. I thought I would try it with towels first to see if the concept worked before shelling out for perlite. Sorry to sound stingy, but it is an expensive hobby!
Home Depot has bigass bags of perlite for $20. Each bag is enough for 4" depth in 3 66qt shotgun chambers.
If you bought RRs videos the shotgun tek is in there. It is halfway through the "Getting Started With BRF Cakes" section. Which is like the first section. That segment is also on youtube.
Jump to 16:15
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AgarStudent
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Re: Shotgun Terrarium Tek [Re: Juiceh]
#23752913 - 10/19/16 07:12 PM (7 years, 3 months ago) |
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Thanks for the details guys 
Would I be right to simply pressure cook some spawn bags filled with:
horse manure/straw/coir/vermiculite/gypsum (40/20/20/15/5). for 2 hours at 15 PSI
..and then add rye berries colonised with chico nindo or galindoi using grain to grain transfer in front of the flow hood?
Or is the proper process of pasteurization going to give me significantly better resistance to contamination?
Cheers
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Juiceh
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Registered: 09/25/12
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How big of a spawn bag are you talking? I'd increase that time to 3 hours at 15psi for a 5+ lb bag. 2 hours would be ok if you have an AA sterilizer or AA PC modded with a sterilizer valve and can run 20psi.
If you can spawn a sterilized bag of substrate aseptically and seal it in front of a flow hood you don't need to worry about the contam resistance of pasteurized subs. I don't have data on it, but it's likely that PCing and cooking the sub more breaks things down and makes the nutrients more available for the mycelium.
I sterilize substrate and then spawn it in front of the hood all the time. It requires more equipment and skill, but it's totally doable. It's just not noob friendly or SAB friendly, though it can also be done in a SAB but I would not recomend it.
Edited by Juiceh (10/19/16 10:37 PM)
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AgarStudent
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Re: Shotgun Terrarium Tek [Re: Juiceh]
#23753217 - 10/19/16 08:46 PM (7 years, 3 months ago) |
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Cheers Juiceh
In your last paragraph, you you mean you inoculate the substrate in front of the hood?
Just checked out RR's horse poo prep video too. Awesome
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Juiceh
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Registered: 09/25/12
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Yes. If I sterilize the substrate, I inoculate it in front of the flowhood.
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AgarStudent
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Re: Shotgun Terrarium Tek [Re: Juiceh]
#23754182 - 10/20/16 07:58 AM (7 years, 3 months ago) |
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In RR's video on dung pasteurisation, it doesn't look like he is casing his grains and substrate in front of the flow hood? I guess if you are sterilising everything then it can't hurt, although I wonder how you do that with a bigger tub - say if you are doing it it a 50 or 60-quart mono tub.
I am guessing once the grains are colonised and the bulk substrate is pasteurized, then there is more margin for potential contaminants, due to the 'theory' of heat resistant bugs selectively killing off undesirable moulds.
I am not sure if the pasteurization method is based on solid science that I know of, but I would be happy to learn more about it?
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Juiceh
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Registered: 09/25/12
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You don't need to use a flow hood with pasteurized bulk substrate. The heat resistant bacteria protect it. You can inoculate in open air.
You must inoculate all sterilized substrates in aseptic conditions because they don't have that protection.
If you are sterilizing your substrate you don't use tubs. Once the bags of substrate have been spawned and colonized you trim the top off the bag and apply a casing layer after full colonization if you want. Then you put it in your greenhouse\fruiting chamber. Then you get this:


Many gourmet edibles are grown this way. If you can grow cubes this way you're ready to move on to many other gourmet species.
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AgarStudent
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Re: Shotgun Terrarium Tek [Re: Juiceh]
#23755691 - 10/20/16 06:20 PM (7 years, 3 months ago) |
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Okay thanks for clarifying 
If you did sterilise your substrate in the PC (such as poo/verm) then it would follow that you would only be putting it in casing trays (not tubs), as it would be easy to put these trays in front of a hood, while you mix in some spawn and put over some alfoil and poke a few holes for air prior to colonisation.
A week or two later, you would then put the colonised cased tray in front of the flow hood to case it (say with peat/CaCo3), where it would thus be protected from fallout and random contaminants.
You could then take your cased and colonised tray into the humidity chamber, pretty confident that it has some resistance to contamination.
Is that right? Cheers
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Juiceh
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Registered: 09/25/12
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Quote:
AgarStudent said: Okay thanks for clarifying 
If you did sterilise your substrate in the PC (such as poo/verm) then it would follow that you would only be putting it in casing trays (not tubs), as it would be easy to put these trays in front of a hood, while you mix in some spawn and put over some alfoil and poke a few holes for air prior to colonisation.
A week or two later, you would then put the colonised cased tray in front of the flow hood to case it (say with peat/CaCo3), where it would thus be protected from fallout and random contaminants.
You could then take your cased and colonised tray into the humidity chamber, pretty confident that it has some resistance to contamination.
Is that right? Cheers
No. Did you not see what I said about sterilizing in bags? Where did I ever say the substrate was removed from the bags. I said trim the bag and case once colonized, nothing about trays whatsoever. The pictures above show the bags.
If you sterilize in a bag you do not remove it from the bag. The only time you would consider doing that is ONLY after full colonization. Removing a sterilized substrate from its sterile container is asking for disaster. Dumping a bag of sterilized substrate and then mixing grain in by hand(not tumbled in a sealed bag) and then covering with foil and poking holes in it to let contams land on your sterilized(not pasteurized and protected) sub is a guaranteed failure. You do this with pasteurized sub, never sterilized sub.
In aseptic conditions(Flowhood or SAB) you spawn the sterilized bag of substrate with a jar of colonized grain then seal the bag. Tumble the bag and distribute the grains around the substrate without busting the bag. Once its fully colonized in a few weeks you take a razor and trim away the top of the bag so that there is about an inch or two above the substrate surface and apply your pasteurized casing. You do not have to apply the casing in front of the flowhood since the sterilized substrate is now fully colonized.
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AgarStudent
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Re: Shotgun Terrarium Tek [Re: Juiceh]
#23763506 - 10/23/16 08:20 AM (7 years, 3 months ago) |
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Ah okay,
Thanks for fleshing that out. I have actually never pasteurised a substrate before. My last success with cubes was based on using monotubs, in which I was using coir/verm 50/50 and basically sterilised everything except the grain. I never had a contam issue with cubes.
I have heard that pasturised poo substrates are a bit tougher and are working on that one. Hopefully someone can help me out with that - it is a different post.
I can see in your post that you have the spawn bags going - If you just cut the top off them, then your only fruiting that top bit. How do you maximise your yield, by allowing the rest of your colonised substrate to fruit? Does the bit that fruits mean that you cut the bag a bit lower after each flush until all of the substrate is used up?
Cheers
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