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Offlinetommyg57

Registered: 07/05/15
Posts: 140
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question about making swabs
    #23745705 - 10/17/16 02:04 PM (8 years, 3 months ago)

Hey everyone, just a quick and possibly dumb question. About to sterilize some q tips for swabs. I see everyone having some form of gas exchange on the jar with the swabs in them. Is that needed? Will the jar explode? Never PC'd anything that doesn't have some sort of moisture content. Thanks!

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Invisiblewildernessjunkie
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Re: question about making swabs [Re: tommyg57]
    #23745746 - 10/17/16 02:17 PM (8 years, 3 months ago)

It won't explode. But will vacuum seal itself if there's no gas exchange. When you open the jar, it could potentially suck a bunch of dirty air in.

You can flip your jar lid rubber side up, and it will all work out.

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Offlinetommyg57

Registered: 07/05/15
Posts: 140
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Re: question about making swabs [Re: wildernessjunkie]
    #23745764 - 10/17/16 02:23 PM (8 years, 3 months ago)

Ok great, thank you! Ended up punching one 1/4 hole in the lid before I fired everything up.

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Invisiblewildernessjunkie
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Re: question about making swabs [Re: tommyg57]
    #23745774 - 10/17/16 02:25 PM (8 years, 3 months ago)

If its not filtered or sealed somehow, you'll be exposing them to open air and defeating the purpose of sterilization when you remove them from the PC.

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Offlinetommyg57

Registered: 07/05/15
Posts: 140
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Last seen: 7 years, 7 months
Re: question about making swabs [Re: wildernessjunkie]
    #23745794 - 10/17/16 02:30 PM (8 years, 3 months ago)

Foil okay you think..? Honestly didn't think of this process being as severe as grains or agar.

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Offlineenlightenment
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Re: question about making swabs [Re: tommyg57]
    #23745806 - 10/17/16 02:34 PM (8 years, 3 months ago)

Quote:

wildernessjunkie said:
If its not filtered or sealed somehow, you'll be exposing them to open air and defeating the purpose of sterilization when you remove them from the PC.




:whathesaid:

stuff the hole with polyfill.

Where i live you can buy sterile sawbs in the pharmacy for just few dollars. That's what I use.

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Offlinetommyg57

Registered: 07/05/15
Posts: 140
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Last seen: 7 years, 7 months
Re: question about making swabs [Re: enlightenment]
    #23745838 - 10/17/16 02:43 PM (8 years, 3 months ago)

Hmm maybe ill check that instead of pcing these. I started reading these teks yesterday. Honestly had no idea ppl pc'd these. Maybe sterilize like u would for pf tek, substrates etc.. but PC!? Damn, off to the pharmacy in a few.

Edited by tommyg57 (10/17/16 02:48 PM)

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InvisibleMycolorado
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Registered: 07/23/16
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Re: question about making swabs [Re: tommyg57]
    #23745887 - 10/17/16 02:51 PM (8 years, 3 months ago)

Quote:

tommyg57 said:
Foil okay you think..? Honestly didn't think of this process being as severe as grains or agar.



Yep...no need for the lid at all...

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Invisiblemorty422
Wuba-luba-dub-dub!
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Registered: 07/06/16
Posts: 988
Re: question about making swabs [Re: Mycolorado]
    #23745928 - 10/17/16 02:59 PM (8 years, 3 months ago)

I would definitely just find some sterile swabs from a drug store or pharmacy, etc...

They are cheap and sufficiently sterile for swabbing spores to plates.

IMO PCing swabs is sort of a time-waster. I can find sterile swabs for just a few dollars USD where I'm from...

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InvisibleJosex
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Re: question about making swabs [Re: morty422]
    #23745961 - 10/17/16 03:05 PM (8 years, 3 months ago)

And real swabs from the drugstore are much longer than q-tips, letting you keep a safe distance from your plates.
Even if they don't have it at the drugstore, you can ask them to order them for you, real cheap.

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