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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Ferather]
    #23840176 - 11/16/16 09:48 PM (7 years, 2 months ago)

So I'm glad I didn't put the parasol clones in the fridge yet.
Turns out the myc just started growing much more thinly so I didn't see the new growth, rather than completely stalling out as I thought it did originally.
It's thickening up again too!

The polypore is on it's first set of transfers now, still growing quickly.
Mold colonies on the original clone plates have begun sporulating so it's fortunate I got the transfers out before that happened.


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OfflineMattisfat
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23840627 - 11/17/16 03:10 AM (7 years, 2 months ago)

:popcorn:


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OfflineGreg
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Wild mushroom clone logs [Re: Mattisfat]
    #23849097 - 11/19/16 04:10 PM (7 years, 2 months ago)

Pine polypore progress:


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OfflineFerather
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Re: Wild mushroom clone logs [Re: Greg]
    #23849142 - 11/19/16 04:26 PM (7 years, 2 months ago)

How did that happen Greg? Did they fall off your sample do you think?
I am very happy to see you got a clean transfer running.

That mycelium is very strong looking.


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OfflineGreg
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Re: Wild mushroom clone logs [Re: Ferather]
    #23849156 - 11/19/16 04:30 PM (7 years, 2 months ago)

Probably came from the outside of the fruit I cloned, it was living on a rotting pine tree after all :lol:
Doubt I would have got mold if I could have ripped the fruit open to get tissue but it was too dense for that, so I had to cut tissue from the outside of it.


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OfflineFerather
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Re: Wild mushroom clone logs [Re: Greg]
    #23849173 - 11/19/16 04:35 PM (7 years, 2 months ago)

Fair enough, fully noted, thanks  :takingnotes:


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OfflineGreg
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Re: Wild mushroom clone logs [Re: Ferather]
    #23856764 - 11/22/16 10:10 AM (7 years, 2 months ago)

Shaggy Parasol and polypore clones update:


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OfflineMattisfat
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Re: Wild mushroom clone logs [Re: Greg]
    #23856951 - 11/22/16 11:00 AM (7 years, 2 months ago)

That shaggy parasol has some nice colouring on agar


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OfflineFerather
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Re: Wild mushroom clone logs [Re: Mattisfat]
    #23857152 - 11/22/16 12:11 PM (7 years, 2 months ago)

Hmmm, I am worried that colouring is bacteria. It is to far from the mycelium, and far from uniform around it.
Each plate has different patching and there is an obvious difference in colour too.

Or is it really that bad, like a Reishi grow full of bio waste.


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OfflineGreg
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Re: Wild mushroom clone logs [Re: Ferather]
    #23857163 - 11/22/16 12:15 PM (7 years, 2 months ago)

I'll put it under my microscope later and we'll know for certain.

Edit:
All I see at 400x and 1000x is dense brown mycelium, no other microorganisms.
I will post micrographs soon.


Edited by Greg (11/22/16 12:29 PM)


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OfflineFerather
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Re: Wild mushroom clone logs [Re: Greg]
    #23857198 - 11/22/16 12:31 PM (7 years, 2 months ago)



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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineGreg
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Re: Wild mushroom clone logs [Re: Ferather]
    #23857216 - 11/22/16 12:37 PM (7 years, 2 months ago)

I've seen that before actually.
You have to consider where he took that clone, presumably somewhere in Rhode Island which is across the states from me.
Also consider the post right below that picture.
Quote:

Terry, that looks very good, when I've tried to grow this, it was never as symmetrical as yours, and the agar stained dark brown pretty quickly.



Here is 1000x, myc taken from near the center of one of the plates.


Not saying it's not possible for it to be contaminated, it just really doesn't look that way to me.


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OfflineGreg
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Re: Wild mushroom clone logs [Re: Greg]
    #23857289 - 11/22/16 01:02 PM (7 years, 2 months ago)

Couple other pictures.
Agar from the area that's stained brown but doesn't have myc on it:

Agar from a blank petri:

Ignore the stains in the same spots on both photos, some random shit got on the camera adapter lens and I'm not sure how to clean it off...

Edit:
Lastly, here is a bit of agar from a particularly slimy plate:


Edited by Greg (11/22/16 01:21 PM)


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OfflineFerather
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Re: Wild mushroom clone logs [Re: Greg]
    #23857338 - 11/22/16 01:22 PM (7 years, 2 months ago)

Image one has a love heart, around it is a rainbow pool. Like petrol (gasoline) on water.
Image 2 is clear of said formation, indeed it looks chemical and not bacterial.

Although, image one the 3 green dots have the same pools.


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OfflineFerather
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Re: Wild mushroom clone logs [Re: Ferather]
    #23857426 - 11/22/16 01:54 PM (7 years, 2 months ago)

I enhanced your first two images and uploaded to Imgur: Here.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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OfflineGreg
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Re: Wild mushroom clone logs [Re: Ferather]
    #23857455 - 11/22/16 02:02 PM (7 years, 2 months ago)

:neat:

I see the little heart too lol.


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OfflineFerather
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Re: Wild mushroom clone logs [Re: Greg]
    #23857497 - 11/22/16 02:19 PM (7 years, 2 months ago)

After comparing all three images fully enhanced, the pooling although weird is chemical.
Thats as far as I can go, the reasoning for it is beyond me at the moment.

At a stab I would say it's some sort of bio waste, or enzyme.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineShu
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Re: Wild mushroom clone logs [Re: Greg]
    #23857645 - 11/22/16 03:03 PM (7 years, 2 months ago)

Quote:

Greg said:
I've seen that before actually.
You have to consider where he took that clone, presumably somewhere in Rhode Island which is across the states from me.
Also consider the post right below that picture.
Quote:

Terry, that looks very good, when I've tried to grow this, it was never as symmetrical as yours, and the agar stained dark brown pretty quickly.



Here is 1000x, myc taken from near the center of one of the plates.


Not saying it's not possible for it to be contaminated, it just really doesn't look that way to me.




Looks good to me too!


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Invisibleflyontoast
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Re: Wild mushroom clone logs [Re: Ferather]
    #23861101 - 11/23/16 03:52 PM (7 years, 2 months ago)

Quote:

Ferather said:
I enhanced your first two images and uploaded to Imgur: Here.



Did you try pulling-up a concordial bitmap algorithm and just apply it to the horizontal pixels?


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Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors.
Also, any commercially viable Pleurotus, cold or hot strains.
Thanks for the Q&A, trades, and all the posters & teachers that have come before us  :skol:


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