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OfflineGreg
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Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt.
    #23745725 - 10/17/16 02:10 PM (7 years, 3 months ago)

So I recently found several good sized patches of a wonderful edible species called Shaggy Parasol:
https://www.shroomery.org/forums/showflat.php/Number/23740148
The ID was confirmed by a TI.



I took 6 stipe cuttings to agar and so far at least 2 have shown new growth!
There is a fair amount of bacteria but I'm confident I can clean the cultures up.

This species is supposedly culturable by standard methods because it's saprobic.

I'll update this post if anything comes of this.
I hope to do several indoor and outdoor grows with this species, as well as a culture giveaway...


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23757906 - 10/21/16 12:19 PM (7 years, 3 months ago)

Update, 4 plates out of 7 displayed obvious growth from the clone tissue.
Only 2 were free of any contaminants AND showed growth.

Here they are!


This species bruises brown/red so heavily that it actually dyed the agar brown on some of the plates... :eek:


Edited by Greg (10/21/16 12:29 PM)


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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23758006 - 10/21/16 12:54 PM (7 years, 3 months ago)

Nice!  Would you grow them on leaf litter or compost of sorts?


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Mycolorado]
    #23758015 - 10/21/16 12:58 PM (7 years, 3 months ago)

They were growing near a row of windbreak pines (and one large maple tree).
Heavy leaf and pine duff + dirt.

I will probably use a mix of leaf litter, aged pine needles and compost.

I am going to try some CVG and manure grows indoors too.


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23781099 - 10/28/16 07:58 PM (7 years, 3 months ago)

Update:
One of the plates got a colony of bacteria near the edge of the dish, probably from mishandling of the plate during inoculation.
I would usually just toss it, but since I don't have much of this culture I decided to cut the original transfer from the plate and divide it to two new plates.

They fuzzed up right after transfer and look pretty healthy.
So that makes 3 plates total now.

Still growing very slowly.



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Offlinekatbusa
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23781113 - 10/28/16 08:02 PM (7 years, 3 months ago)

Quote:

Greg said:
Update:
One of the plates got a colony of bacteria near the edge of the dish, probably from mishandling of the plate during inoculation.
I would usually just toss it, but since I don't have much of this culture I decided to cut the original transfer from the plate and divide it to two new plates.

They fuzzed up right after transfer and look pretty healthy.
So that makes 3 plates total now.

Still growing very slowly.






Very cool!


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OfflineFerather
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: katbusa]
    #23782237 - 10/29/16 10:06 AM (7 years, 3 months ago)

Got any white paper? Bottom left looks like it has a dark ring around it.
Top middle also looks like there may be a dark bit to the left.


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Ferather]
    #23782243 - 10/29/16 10:09 AM (7 years, 3 months ago)

They definitely do have a dark ring around the tissue samples!
Good eye.

Reason for the coloration is this species brusies red/dark brown if cut.
The bruising is so strong it actually leaches out in to the agar.

The reason the bottom left has more brown to it is because it's still on the original transfer plate, the others have been moved to new agar.

Pretty neat. Surprised me at first.


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OfflineFerather
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23782247 - 10/29/16 10:11 AM (7 years, 3 months ago)

Oh nice, fair enuff.


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Ferather]
    #23783658 - 10/29/16 07:55 PM (7 years, 3 months ago)

I found the old specimen of this species I collected almost 600 feet from the current patch.
Left is the old one, right is the new one.


I collected the old one about 2 years ago.


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23806628 - 11/06/16 01:29 PM (7 years, 2 months ago)

Still growing extremely slowly...


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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23806915 - 11/06/16 02:53 PM (7 years, 2 months ago)

The top one sure has nice growth, though.  Have you tried different recipes to see if it responds better to varied nutrient sources? i.e. MEA, DFA, etc...just curious...I know you know your shit.


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Mycolorado]
    #23807083 - 11/06/16 03:15 PM (7 years, 2 months ago)

Not yet. I want to try peptone as an additive though.


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OfflineFerather
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23807114 - 11/06/16 03:25 PM (7 years, 2 months ago)

Interesting move Greg, I follow. I have noticed myself, I get similar results with Gelatin.
Gelatin, tea and water has the same effect as agar, starch, tea and water.

Would need to side-by-side, but its interesting.


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Ferather]
    #23816487 - 11/09/16 02:53 PM (7 years, 2 months ago)

I found a wild polypore just out of the pin stage, it was growing from a recently dead lodgepole pine.
Took it to 6 plates of agar. (4 gent+MEA, 2 MEA)
Unfortunately I couldn't break it open to get at clean tissue because the thing was so damn dense!
I ended up just cutting bits with a scalpel, it wasn't easy.
It smells like perfume and mushrooms, it's bizarre.

This is now a C. rhacodes and a wild polypore clone log...


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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg] * 1
    #23816750 - 11/09/16 04:17 PM (7 years, 2 months ago)

Good Luck, From Ferather  :thumbup:


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Ferather]
    #23829030 - 11/13/16 12:37 PM (7 years, 2 months ago)

The Shaggy Parasol plates have slowed to almost a halt, definitely need something they aren't getting from plain MEA.
I'm going to pop 'em in the fridge and work on them at a later date...
Might try Ferather's tea stuff, peptone agar, etc...

The polypore is showing lots of growth already.
Plenty of bacteria too but it's clean enough to get a good culture:



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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23829400 - 11/13/16 02:32 PM (7 years, 2 months ago)

What is the "gent" mean in gent+MEA agar?
Also, what kind of polypore is that? That brown bruising could be ganoderma applanatum, are you in the Pacific North West of NA? Is the top kind of a pale, dusty brown?


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OfflineGreg
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: flyontoast]
    #23829411 - 11/13/16 02:35 PM (7 years, 2 months ago)

gent = gentamicin = autoclavable antibiotic

No idea what polypore it is, far too young to tell for sure.
Only reason I know it's a polypore is it had already developed a small pore surface on one side.
It smells like perfume and was found on a lodgepole pine.
Slight brown bruising.
I'm in Montana, temps here are dipping below freezing every few nights.

P.S. If anyone wants a sample of this culture PM me.


Edited by Greg (11/13/16 02:53 PM)


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OfflineFerather
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Re: Chlorophyllum rhacodes aka Shaggy Parasol, clone attempt. [Re: Greg]
    #23829602 - 11/13/16 03:35 PM (7 years, 2 months ago)



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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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