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OfflineSouth_Paw
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Registered: 07/10/14
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Re: Do I need really need a PC? [Re: PussyFart] * 2
    #24114493 - 02/23/17 08:06 PM (6 years, 11 months ago)

Why do you like agar mushboy?
I think I already know the answer, but I am here to hear opinions.
For me, it's the ability you have to isolate strains... It is no doubt more precise than trying to use LC, but LC can still be used in the same way. If not much less efficient.

Quote:

NDStepp84 said:
Let me send you some of my high endospore oats and try to just PC them one hour @ 15 PSI, they will think it's just a sunny day at the beach.




Who said anything about oats? I'd love to try it though

Quote:

mushboy said:
I eat raw red meat. I love tenderloin shaved thin with lemon juice salt n pepper. Mmmm
Nature has symbiotic relationships. A micro climate inside a dwelling does not




I agree. Steak is delicious, I get mine rare. Love sushi too. Everyone likes their food a little raw sometimes. How would you feel if you ate burnt toast everyday?

Quote:

PussyFart said:
Quote:

South_Paw said:
Well, I've posted my results and I have no reason to lie to you.



Well, others have posted their results and we have no reason to believe you....

Grains for 15 min @ 13 psi :smbfacepalm:




Again fair enough. My grows will be well documented from now on.

So does anyone want to comment of the tek or not?


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Offlinetump
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Registered: 03/17/16
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Re: Do I need really need a PC? [Re: South_Paw]
    #24114895 - 02/23/17 11:09 PM (6 years, 11 months ago)

I agree a little bit my first 20 or so bacths of grain were pc for 90 mins instead of 2 hours or 2.5 hours. And they were sterilize enough to live with. Then i reliezied that advice changed for a reason. The stardred is 2 hours that kills 14% more endospores. Running a pc another hour on a 1000w stove or my case two pc then for hour is 15 cents. Isn't your piece of mind per grain run worth that to you. Since i swicthed over to two hours pc time I've only shut it off earlier once because i was passing out and re cooked it the next day.


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Offlinetump
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Re: Do I need really need a PC? [Re: South_Paw]
    #24114914 - 02/23/17 11:18 PM (6 years, 11 months ago)

Quote:

South_Paw said:
Why do you like agar mushboy?
I think I already know the answer, but I am here to hear opinions.
For me, it's the ability you have to isolate strains... It is no doubt more precise than trying to use LC, but LC can still be used in the same way. If not much less efficient.

Quote:

NDStepp84 said:
Let me send you some of my high endospore oats and try to just PC them one hour @ 15 PSI, they will think it's just a sunny day at the beach.




Who said anything about oats? I'd love to try it though

Quote:

mushboy said:
I eat raw red meat. I love tenderloin shaved thin with lemon juice salt n pepper. Mmmm
Nature has symbiotic relationships. A micro climate inside a dwelling does not




I agree. Steak is delicious, I get mine rare. Love sushi too. Everyone likes their food a little raw sometimes. How would you feel if you ate burnt toast everyday?

Quote:

PussyFart said:
Quote:

South_Paw said:
Well, I've posted my results and I have no reason to lie to you.



Well, others have posted their results and we have no reason to believe you....

Grains for 15 min @ 13 psi :smbfacepalm:




Again fair enough. My grows will be well documented from now on.

So does anyone want to comment of the tek or not?



Ok so heres a tek. You make agar plates and cook them 45 mins. You drop spores on them , you make tranfers until clean all in still air box. You tranfer clean into a cooked lc in still air boox. You prep grain, soaking 24 hours and drain to air dry 1 hour. You load up jars with aready made lids with ship and ge filter, cover in foil. Pc 2 hours at 15psi. Let cool down. Knock up sterility with syringes pc in same cycle and suck up lc. Inject into grain jar small amount. Repeat each day with new jars for new grain. Once colonized grain jara are done, mix coir in a bucket with water and then mix in grain and drump in pastic tote with lid cracked. Mist when needed, pick shrooms. Repeat, repeat, repeat. Done a tek for you


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InvisiblemushboyMDiscord
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Re: Do I need really need a PC? [Re: tump]
    #24114935 - 02/23/17 11:29 PM (6 years, 11 months ago)

No I mean carpaccio. Not rare. But raw.
I'm super duper cereal.


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Offlineknorr
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Registered: 02/20/17
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Last seen: 6 years, 4 months
Re: Do I need really need a PC? [Re: Mad Season]
    #24115126 - 02/24/17 01:43 AM (6 years, 10 months ago)

I just wanted an elaboration really..to clear things up for me as well as for others.

I assumed you were talking about LC's inoculated with a clean agar wedge, because MS syringe -> LC seems to be a certain way to fail and did not even occur to me as an alternative. Also the OP says his LC is clean.

Id never inoculate grains with a ms syringe, mainly because I don't like to wait for failure when I can wait for results, and wouldn't advocate it.

Cleaning ones inoculum is easy and encouraging people to use dirty inoculum seems to be a disfavour imo.


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Offlinetump
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Re: Do I need really need a PC? [Re: knorr]
    #24115148 - 02/24/17 02:11 AM (6 years, 10 months ago)

Quote:

knorr said:
I just wanted an elaboration really..to clear things up for me as well as for others.

I assumed you were talking about LC's inoculated with a clean agar wedge, because MS syringe -> LC seems to be a certain way to fail and did not even occur to me as an alternative. Also the OP says his LC is clean.

Id never inoculate grains with a ms syringe, mainly because I don't like to wait for failure when I can wait for results, and wouldn't advocate it.

Cleaning ones inoculum is easy and encouraging people to use dirty inoculum seems to be a disfavour imo.



Yes clean agar wedge into lc.


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Offlineknorr
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Registered: 02/20/17
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Re: Do I need really need a PC? [Re: tump]
    #24115269 - 02/24/17 04:45 AM (6 years, 10 months ago)

Quote:

tump said:
Yes clean agar wedge into lc.




I wouldn't do anything without knowing the inoculate is clean.

Surprised a TC would do otherwise.


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InvisibleMad Season
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Re: Do I need really need a PC? [Re: knorr]
    #24115368 - 02/24/17 06:32 AM (6 years, 10 months ago)

Yeah agar is the best but I assumed he didn't have agar, considering he didn't have a PC, and sounded like this was his first time doing grains.. I automatically don't trust anybody saying their LC is clean at that point. Even if it grew in brf.. bacteria doesn't affect brf nearly as bad as it does in grain spawn.

So yeah 1 drop of spores to grains is way better than a noob made LC, unless you can do agar well.. in which case for practice dropping wedges to grains and g2ging is IMO better for noobs than LC anyways.


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Edited by Mad Season (02/24/17 06:38 AM)


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Offlineknorr
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Re: Do I need really need a PC? [Re: Mad Season]
    #24117926 - 02/25/17 03:33 AM (6 years, 10 months ago)

Quote:

Mad Season said:
Yeah agar is the best but I assumed he didn't have agar, considering he didn't have a PC, and sounded like this was his first time doing grains.. I automatically don't trust anybody saying their LC is clean at that point. Even if it grew in brf.. bacteria doesn't affect brf nearly as bad as it does in grain spawn.

So yeah 1 drop of spores to grains is way better than a noob made LC, unless you can do agar well.. in which case for practice dropping wedges to grains and g2ging is IMO better for noobs than LC anyways.




I just took his word for the LC being clean. Perhaps I'm weird in that way, but others like me could misinterpret your wording and use ms to grains because a trusted cultivator said so.
I just care about the success people have with their cultivation. Wasn't out to get you personally. 

If what the OP said about his LC isn't the case I agree 100% with your reasoning.


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Offlinetump
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Re: Do I need really need a PC? [Re: knorr]
    #24118038 - 02/25/17 05:45 AM (6 years, 10 months ago)

Everyone says there of is clean until they see contam hit the jar or sub before it fruits. Even my last of big batch should have been clean since I used a clean wedge but all 49 jars knocked up were bacteria infection and made vingar smelling jars. Its best to test this out but until you see fruit your never 100% sure you did good. But same plate of agar I knocked up different jar of of at same time and spawn looks and smells fine. Lc is like a sword you live by Lc you die of lc


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Offlineblackout
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Re: Do I need really need a PC? [Re: South_Paw] * 1
    #24118851 - 02/25/17 01:28 PM (6 years, 10 months ago)

Quote:

South_Paw said:
yet i'm still confused as to why people say to PC for over an hr. just seems like a waste of time and dries out your substrate.



yep, the forum is full of non stop threads with people with useless dry grains after PCing for over 60mins, to think all these idiots had to do was reduce the time to 13mins. You'd think they would have seen all the other threads and know it would be a total failure.

I really share your confusion...


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Offlineknorr
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Re: Do I need really need a PC? [Re: blackout]
    #24120345 - 02/26/17 03:36 AM (6 years, 10 months ago)

Quote:

blackout said:
Quote:

South_Paw said:
yet i'm still confused as to why people say to PC for over an hr. just seems like a waste of time and dries out your substrate.



yep, the forum is full of non stop threads with people with useless dry grains after PCing for over 60mins, to think all these idiots had to do was reduce the time to 13mins. You'd think they would have seen all the other threads and know it would be a total failure.

I really share your confusion...




Let me clear that confusion for you mate. We pc for 90min because we want to raise the RH in the kitchen :grin:

Edit: and that's why AA pressure cookers suck :tongue:


Edited by knorr (02/26/17 03:38 AM)


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OfflineTiamo
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Re: Do I need really need a PC? [Re: knorr]
    #24120436 - 02/26/17 05:24 AM (6 years, 10 months ago)

Nothing better than a 2 hour PC run in winter. Free sauna


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OfflineSouth_Paw
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Registered: 07/10/14
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Re: Do I need really need a PC? [Re: blackout]
    #24120858 - 02/26/17 09:59 AM (6 years, 10 months ago)

Quote:

blackout said:
Quote:

South_Paw said:
yet i'm still confused as to why people say to PC for over an hr. just seems like a waste of time and dries out your substrate.



yep, the forum is full of non stop threads with people with useless dry grains after PCing for over 60mins, to think all these idiots had to do was reduce the time to 13mins. You'd think they would have seen all the other threads and know it would be a total failure.

I really share your confusion...




:eek: the amount of people who post without reading the entire thread is fairly mind-boggling. Sometimes this place reminds me of the comments on Yahoo answers.

I never had a problem with dry grains, I presoak and cook. I'm talking about other types of materials.


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OfflineSouth_Paw
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Registered: 07/10/14
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Re: Do I need really need a PC? [Re: Mad Season]
    #24120886 - 02/26/17 10:15 AM (6 years, 10 months ago)

Quote:

Mad Season said:
Yeah agar is the best but I assumed he didn't have agar, considering he didn't have a PC, and sounded like this was his first time doing grains.. I automatically don't trust anybody saying their LC is clean at that point. Even if it grew in brf.. bacteria doesn't affect brf nearly as bad as it does in grain spawn.

So yeah 1 drop of spores to grains is way better than a noob made LC, unless you can do agar well.. in which case for practice dropping wedges to grains and g2ging is IMO better for noobs than LC anyways.




Also, being a noob and being stupid are two different things. My LC is clean. And i favor it over agar because I can literally have gallons of clean LC. Its so much more versatile. But agar is fun too. Id like to make some agar LC someday


--------------------

"The most successful people I know are lazy opportunists."


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InvisiblebodhisattaMDiscordReddit
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Re: Do I need really need a PC? [Re: South_Paw]
    #24120890 - 02/26/17 10:19 AM (6 years, 10 months ago)

Quote:

South_Paw said:My LC is clean. And i favor it over agar because I can literally have gallons of clean LC. Its so much more versatile. But agar is fun too. Id like to make some agar LC someday



:lolwut:

Quote:

South_Paw said:
yet i'm still confused as to why people say to PC for over an hr. just seems like a waste of time and dries out your substrate.



:lolwut:X2


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InvisibleMr Piggy
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Re: Do I need really need a PC? [Re: bodhisatta]
    #24120895 - 02/26/17 10:20 AM (6 years, 10 months ago)

I'm just sitting here waiting to see these clean grains.

:shrug:


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InvisibleMycolorado
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Re: Do I need really need a PC? [Re: South_Paw]
    #24120899 - 02/26/17 10:21 AM (6 years, 10 months ago)

Quote:

South_Paw said:
I'm trying to inoculate half pint jars of rye berries with LC (clean). However I've been reading that steam sterilization doesn't kill endospores therefore I need a PC for sterilization if I'm trying to inoculate grains.

So any opinions on this? Or should I just go ahead and steam sterilize and shoot em up?





Quote:

South_Paw said:
Just'd like to bump this. ended up buying a PC for about $100. Sterilizes BRF rye , manure, peat, etc just fine in as little as 15 mins (PSI ~ 13).




What's all this?


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Offlineblackout
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Re: Do I need really need a PC? [Re: South_Paw]
    #24120908 - 02/26/17 10:25 AM (6 years, 10 months ago)

Quote:

South_Paw said:
the entire thread is fairly mind-boggling.


:thumbup:


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OfflineSouth_Paw
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Registered: 07/10/14
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Re: Do I need really need a PC? [Re: Mycolorado]
    #24120953 - 02/26/17 10:39 AM (6 years, 10 months ago)

Quote:

Mycolorado said:
Quote:

South_Paw said:
I'm trying to inoculate half pint jars of rye berries with LC (clean). However I've been reading that steam sterilization doesn't kill endospores therefore I need a PC for sterilization if I'm trying to inoculate grains.

So any opinions on this? Or should I just go ahead and steam sterilize and shoot em up?





Quote:

South_Paw said:
Just'd like to bump this. ended up buying a PC for about $100. Sterilizes BRF rye , manure, peat, etc just fine in as little as 15 mins (PSI ~ 13).




What's all this?




That was the original topic of the thread, which seems to have expanded to another question about sterilization time


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"The most successful people I know are lazy opportunists."


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