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OfflinePussyFart
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Re: Do I need really need a PC? [Re: South_Paw]
    #24112418 - 02/23/17 12:10 AM (6 years, 11 months ago)

Quote:

South_Paw said:
Just'd like to bump this. ended up buying a PC for about $100. Sterilizes BRF rye , manure, peat, etc just fine in as little as 15 mins (PSI ~ 13). I grow everything starting with PF tek, not contams so far in any jars I've done. PC definitely worth it.

Boiling definitely does the job for the most part, rye grain included. I believe the most important part is  sterility during inoculation



Quote:

South_Paw said:
^^^

yet i'm still confused as to why people say to PC for over an hr. just seems like a waste of time and dries out your substrate.



Quote:

South_Paw said:
Also why I like LC. If it's done right it will be sterile and also hydrate substrate when u inoculate.



I need a third hand to facepalm right now......

We say PC BRF jars for 60 minutes and grains for 90 minutes(and these are minimums) because thats how long it takes the center of the substrate jars to come to temperature to ensure proper sterilization and to ensure the death of endospores.

And please explain to me how anything can dry out when the PC is filled with steam....I am dying to hear this one. Me and everyone else who has been doing it for decades must not know that our jars are dried out after 2 hours of sterilization in a 100% RH environment.....I guess I missed that part in basic logic.


Edited by PussyFart (02/23/17 12:12 AM)


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Offlinetump
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Re: Do I need really need a PC? [Re: PussyFart]
    #24112541 - 02/23/17 02:38 AM (6 years, 11 months ago)

After my first steam sterilizer run cooking 24 hours that top grains dried out in all jars in all 4 rows. So it can dry out while cooking. Point being it needs to be cooked proper timed to kill everything even with pf cakes. I don't know why you hate lc pussyfart? If it's clean being knocked up with agar there is nothing faster way off production of clean cultures.


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InvisibleAlexSmitt
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Re: Do I need really need a PC? [Re: tump]
    #24112562 - 02/23/17 03:05 AM (6 years, 11 months ago)

I feel like no one mentioned you can boil/steam brf jars because the fact that there are no endospores there whereas grains do and it takes longer to kill them, hence longer pc time. Like the tc said, those are minimum times. I did read somewhere that you can boil grain for x amount of minutes 3 days in a row and that works for those without a pc. And just for fun I experimented with boiling time on the pf jars, I got away with 25 mins and had great success (which I do not recommend, just follow the tek). As far as drying out, I've thankfully never experienced that.


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InvisibleJosex
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Re: Do I need really need a PC? [Re: tump]
    #24112613 - 02/23/17 04:19 AM (6 years, 11 months ago)

Quote:

tump said:
Pressure cooking makes it easier with small amounts. Streaming makes sense for farms because doing 200lbs of grains and 40 sub blocks is needed for a day production. Get the cheap 70 dallor ones at walmart. As to ms being better then lc is easy. Lc saves time if clean and full of myc. Most sugar lc can't contain more then 12.3% by volume. Most times you can't ready get lc perfectly clean without agar and other thing you need higher psi sterile stuff. The result is myc growing in your liquid with heavt amounts of bacterial life. You shoot up with the bacterial life inside the lc into grains. Boom of bacterial life in grain spawn making myc weaker and slower growth vs even spores water with light bacteria infection. Where once the spores germinate they soon out run the bacterial life.




"You shoot up with the bacterial life inside the lc..."  epic poetry:awepreciation:


Edited by Josex (02/23/17 04:45 AM)


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Offlinetump
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Re: Do I need really need a PC? [Re: Josex]
    #24112639 - 02/23/17 04:57 AM (6 years, 11 months ago)

How long have you been waiting to quote that.


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Invisibleamidogen
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Re: Do I need really need a PC? [Re: Josex]
    #24112678 - 02/23/17 05:53 AM (6 years, 11 months ago)

:highfive:


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Offlineknorr
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Re: Do I need really need a PC? [Re: Mad Season] * 1
    #24112721 - 02/23/17 06:33 AM (6 years, 11 months ago)

Quote:

Mad Season said:
:shrug: I agree with what you said lol

Although MS IMO is better than LC...




As I see it a syringe from a vendor is far more likely to be contaminated than a LC I've made myself. Would think that a TC as yourself would feel the same so that's why I assume you make your own prints in a sterile environment.

Would you recommend a noob to shoot up their grains with a syringe from a vendor? Cuz that's how I would have interpreted your post a couple of years ago..


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InvisibleMad Season
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Re: Do I need really need a PC? [Re: knorr]
    #24113005 - 02/23/17 09:32 AM (6 years, 11 months ago)

What are you asking?

The reason why I feel MS is better than LC is because in a spore syringe, if there's bacteria it doesn't just multiply rapidly and take over all the inoculant. A spore syringe can have a few bacterial drops, but not every drop will be contaminated. An LC is pretty much either clean or fucked. And it can be hard for people to tell if it's bacterial. If you don't have agar, 100% MS > grains is much better than MS > LC > grains.

I'm sure people can make clean LCs with spores, but the point is that unless you're inoculating with agar, it's more risky than just spores by themselves.


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OfflineSouth_Paw
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Re: Do I need really need a PC? [Re: PussyFart]
    #24113241 - 02/23/17 10:58 AM (6 years, 11 months ago)

Quote:

And please explain to me how anything can dry out when the PC is filled with steam....I am dying to hear this one. Me and everyone else who has been doing it for decades must not know that our jars are dried out after 2 hours of sterilization in a 100% RH environment.....I guess I missed that part in basic logic.




Wrong. It most certainly does happen. Learn some thermodynamics.

EDIT: Yet I'm sure that the amount of water in substrate before PCing and the composition of the substrate are a major factor in whether or not it loses moisture. Think about what happens when your PC shuts off. The jars retain heat way longer than the surrounding atmosphere within the PC. Now think about the relationship between cold and hot air.


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Edited by South_Paw (02/23/17 11:07 AM)


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InvisiblemushboyMDiscord
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Re: Do I need really need a PC? [Re: South_Paw]
    #24113250 - 02/23/17 11:02 AM (6 years, 11 months ago)

i just got done pcing about 40lbs of birdseed over 3days. its totally awesome.

it all is pressure cooked at 15 psi for 2hours. this is a industry standard that
people do everyday here all day 365 for 20years.


not an outlandish opinion.


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OfflineSouth_Paw
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Re: Do I need really need a PC? [Re: mushboy]
    #24113268 - 02/23/17 11:10 AM (6 years, 11 months ago)

Well it goes without being said that a larger amount of substrate requires a longer sterilization time .... Now that is common logic. No need to PC half pint BRF tek jars for over an hour, what a waste of time lol


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OfflineSouth_Paw
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Re: Do I need really need a PC? [Re: South_Paw]
    #24113280 - 02/23/17 11:15 AM (6 years, 11 months ago)

Also, who here has taken a steaming hot shower and afterwords had dry skin. Just sayin'


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Invisibleamidogen
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Re: Do I need really need a PC? [Re: South_Paw]
    #24113296 - 02/23/17 11:24 AM (6 years, 11 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (06/21/18 11:01 AM)


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OfflineSouth_Paw
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Re: Do I need really need a PC? [Re: amidogen]
    #24113297 - 02/23/17 11:25 AM (6 years, 11 months ago)

Regardless, my point is still valid.


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Invisibleamidogen
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Re: Do I need really need a PC? [Re: South_Paw]
    #24113302 - 02/23/17 11:27 AM (6 years, 11 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (06/21/18 11:00 AM)


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InvisibleMycolorado
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Re: Do I need really need a PC? [Re: South_Paw]
    #24113313 - 02/23/17 11:33 AM (6 years, 11 months ago)

What's the point of this thread?  What are your results doing it this way?  It just seems like you have the answers you want already, aren't interested in listening to any other input and are arguing about trivial shit.


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OfflineSouth_Paw
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Re: Do I need really need a PC? [Re: amidogen]
    #24113317 - 02/23/17 11:36 AM (6 years, 11 months ago)

That it is indeed possible to dry out substrate by over PCing. And I don't "think" that I have sterilized grains, it addition to basically anything else that would be sterilized , I HAVE sterilized them in that timeframe and not once had issues with contamination.

Not pulling anyone's leg here. Just stating facts.

As for my point in this thread, it's that you really don't need to PC for as long as most of the teks on these threads say.


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InvisibleMycolorado
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Re: Do I need really need a PC? [Re: South_Paw]
    #24113322 - 02/23/17 11:37 AM (6 years, 11 months ago)



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Invisibleamidogen
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Re: Do I need really need a PC? [Re: South_Paw]
    #24113324 - 02/23/17 11:39 AM (6 years, 11 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (06/21/18 11:00 AM)


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OfflinePaulWall
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Re: Do I need really need a PC? [Re: Mycolorado]
    #24113327 - 02/23/17 11:40 AM (6 years, 11 months ago)

There are several threads on this exact same subject. Do some searches


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