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Gizmoleon
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Registered: 07/08/16
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Possible to make some kind of slurry without a blender?
#23745386 - 10/17/16 12:00 PM (7 years, 3 months ago) |
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Hello fellow shroomerites! I'm planning to inoculate some rye jars. I thought of doing it by agar slants, but I think it goes a little slow. Do you think it's possible to cut an partially colonized (and offcourse) isolated agar plate into small pieces and drop it into a container with sterile water (maybe some honey or another nutrient?), shake it, perhaps wait a few days and extract the liquid which by now hopefully will by loaded by nice myc?
Do you have any other tips to make an fast acting medium for inoculation of grain jars? I know G2G and water from colonized grains works wonders, but I have neither at the time.
-------------------- "Who says it's a Camberwell Carrot? I do. I invented it in Camberwell, and it looks like a carrot."
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Josex
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Registered: 11/13/15
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Re: Possible to make some kind of slurry without a blender? [Re: Gizmoleon]
#23745407 - 10/17/16 12:10 PM (7 years, 3 months ago) |
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https://www.shroomery.org/forums/showflat.php/Number/22833314#22833314 https://www.shroomery.org/forums/showflat.php/Number/23188000
I know that the way things are done in these links can be counter-intuitive for a noob, but that's the way most people do it nowadays. It's effective, simple and little risk.
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
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Re: Possible to make some kind of slurry without a blender? [Re: Gizmoleon]
#23745440 - 10/17/16 12:21 PM (7 years, 3 months ago) |
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Tiger drop tic tac toe can be just as fast as liquid
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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Gizmoleon
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Registered: 07/08/16
Posts: 23
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Re: Possible to make some kind of slurry without a blender? [Re: NDStepp84]
#23745825 - 10/17/16 02:40 PM (7 years, 3 months ago) |
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Thank you good sir's! I'm definitely gonna try the tic tac-tec Also gonna try the blenderless li for the hell of it, but my Agar is quite solid. I used the LME recipe. 5g LME and 4,5g agar to 250ml water. I'm not convinced the agar will dissolve in the water, but maybe its no biggie if i shake the living hell out of it? Any experience?
-------------------- "Who says it's a Camberwell Carrot? I do. I invented it in Camberwell, and it looks like a carrot."
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Josex
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Re: Possible to make some kind of slurry without a blender? [Re: Gizmoleon]
#23745869 - 10/17/16 02:49 PM (7 years, 3 months ago) |
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Quote:
Gizmoleon said: Thank you good sir's! I'm definitely gonna try the tic tac-tec Also gonna try the blenderless li for the hell of it, but my Agar is quite solid. I used the LME recipe. 5g LME and 4,5g agar to 250ml water. I'm not convinced the agar will dissolve in the water, but maybe its no biggie if i shake the living hell out of it? Any experience?
The second link I gave you is about how to make an agar mix soft enough to break easily, while at the same time being good for spore inoculation (spores like soft agar) and taking transfers.
It's actually good for your cultures to change your agar recipe from time to time, this will help to keep them vigorous. So it's ok to switch from LME to PDA.
If for whatever reason you need to make an LI and the agar is very stiff for the blenderless, you can use a stainless wire tangled up to make a ball, like in the picture:

That thingy will destroy any agar puck.
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blackout


Registered: 07/16/00
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Re: Possible to make some kind of slurry without a blender? [Re: Josex]
#23746063 - 10/17/16 03:29 PM (7 years, 3 months ago) |
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Quote:
Josex said: If for whatever reason you need to make an LI and the agar is very stiff for the blenderless, you can use a stainless wire tangled up to make a ball, like in the picture:

That thingy will destroy any agar puck.
Commercial versions of these are available, for mixing up dry protein powders, the type weightlifters use.
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Josex
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Re: Possible to make some kind of slurry without a blender? [Re: blackout]
#23746084 - 10/17/16 03:35 PM (7 years, 3 months ago) |
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I know I know! I was so stoked up to buy one of those that I actually emailed the seller of the original "blender bottle" and asked him if all the parts of the blender bottle were made out of pp5 plastic. Only the bottle is, neither lid nor spout are pp5. And I think the same goes for all the different brands available.
I think I read eautu saying that he had a blender bottle and even though the lid is not pp5 it didn't melt after pc'ing, but how long would it take until it deforms?
If you know of a brand which sells these bottles entirely made of pp5, please let me know. I'm like crazy trying to find one.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
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Re: Possible to make some kind of slurry without a blender? [Re: Josex]
#23746197 - 10/17/16 04:10 PM (7 years, 3 months ago) |
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Quote:
Josex said: I know I know! I was so stoked up to buy one of those that I actually emailed the seller of the original "blender bottle" and asked him if all the parts of the blender bottle were made out of pp5 plastic. Only the bottle is, neither lid nor spout are pp5. And I think the same goes for all the different brands available.
I think I read eautu saying that he had a blender bottle and even though the lid is not pp5 it didn't melt after pc'ing, but how long would it take until it deforms?
If you know of a brand which sells these bottles entirely made of pp5, please let me know. I'm like crazy trying to find one.
I have 15 of the blender bottles modified with blender blade attachments glued into the bottom.
I have used them over 20x without any problem as far as warping or melting.
I only pc them for 40 min at most though.
The only problem I have with them is the pour spout is very hard to open so I often just take off the lid to inoculate. I have been taping the pour spout cover to where it is just barely open and then shutting all the way(I have holes with sfd's on the lids for ge during sterilization) so that when I open it with a lot of force in sterile conditions it doesn't go clumsily flying open and possibly contamming the whole thing.
I do love slurries and LI though and these container work great.
For a blenderless LI with stiff agar I think you will want to use something sharper (and maybe heavier)for breaking up the agar than what comes with the shake/blender bottles.
but...
Tic Tac Toe the bitch FTW
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Josex
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Re: Possible to make some kind of slurry without a blender? [Re: tombosley8]
#23746240 - 10/17/16 04:19 PM (7 years, 3 months ago) |
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Really? You're da boss man thanks That's what I wanted to hear, I'm ordering some like right now.
Just to be sure, yours are the original brand "blender bottle"?
LI vs tic-tac-toe... Haven't tried the latter, but all I can think about is that with a single plate you can noc lots of jars if you do a LI, that alone wins it for me
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tombosley8
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Registered: 10/14/13
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Re: Possible to make some kind of slurry without a blender? [Re: Josex]
#23746277 - 10/17/16 04:28 PM (7 years, 3 months ago) |
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I agree with blenderless LI being better than tic tac toe, but just for this case since the agar is solid as he said it will be very hard to break it up very much but I guess it could still be more than a tic tac toe. It's just an extra step that may not have much benefit.
Blenderless LI's work best when the agar is prepared specifically for the task(softer agar)
Yeah my blender bottles are the brand "Blender Bottles"
I have the large and small ones for different procedures such as slurry/LI's.
Also if your attaching the blade use a shitload of red rtc and sand the edges down so it bonds to the surface better.
I originally made a few as EYA shows in the tribal container tek and most ended up leaking after a couple runs.
probably just my shitty craftsmanship but, if your going to be making it, make sure it'll last, right?
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Edited by tombosley8 (10/18/16 09:54 AM)
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Josex
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Re: Possible to make some kind of slurry without a blender? [Re: tombosley8]
#23746310 - 10/17/16 04:36 PM (7 years, 3 months ago) |
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Ok cool and again thank you I'll just buy the bottle and leave it "as is" but for the sfd's as you suggested. I kinda like the ball and shaking for the fuck of it
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Smoothcat
Renegade-master



Registered: 10/07/15
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Re: Possible to make some kind of slurry without a blender? [Re: tombosley8]
#23746355 - 10/17/16 04:45 PM (7 years, 3 months ago) |
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I used a similar agar recipe as yours and blenderless li worked fine. Just use less water and it breaks up better.
There were a few chunks and it wasn't like a perfect slurry but they all worked as inoculation points and colonised quickly.
I like blenderless LI nice and low tek easy to do and as long as your plate is clean you're good 
Good luck!!!
-------------------- Back once again with the ill behaviour Links I like
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
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Re: Possible to make some kind of slurry without a blender? [Re: Smoothcat]
#23747011 - 10/17/16 07:51 PM (7 years, 3 months ago) |
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I bet Eat's corn meal mush plates would kick ass for blenderless LI, haven't read enough of the thread to see if anyone has tried it, but I love cornmeal LI.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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Josex
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Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Possible to make some kind of slurry without a blender? [Re: NDStepp84]
#23747594 - 10/17/16 11:35 PM (7 years, 3 months ago) |
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Quote:
NDStepp84 said: I bet Eat's corn meal mush plates would kick ass for blenderless LI, haven't read enough of the thread to see if anyone has tried it, but I love cornmeal LI.
Amen to that... I've done it with brf plates and one of them was very hard but that nasty wire ball destroyed it The difference in colonization times is really noticeable. Gotta try cornmeal soon was well!
Edited by Josex (10/17/16 11:43 PM)
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wtfcrazymofo
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Re: Possible to make some kind of slurry without a blender? [Re: Gizmoleon]
#23747828 - 10/18/16 02:42 AM (7 years, 3 months ago) |
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Quote:
Gizmoleon said: Hello fellow shroomerites! I'm planning to inoculate some rye jars. I thought of doing it by agar slants, but I think it goes a little slow. Do you think it's possible to cut an partially colonized (and offcourse) isolated agar plate into small pieces and drop it into a container with sterile water (maybe some honey or another nutrient?), shake it, perhaps wait a few days and extract the liquid which by now hopefully will by loaded by nice myc?
Do you have any other tips to make an fast acting medium for inoculation of grain jars? I know G2G and water from colonized grains works wonders, but I have neither at the time.
Tribal container FTW
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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blackdust

Registered: 02/28/09
Posts: 8,327
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Re: Possible to make some kind of slurry without a blender? [Re: wtfcrazymofo]
#23748922 - 10/18/16 01:21 PM (7 years, 3 months ago) |
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i colonize a 4 oz cake drop in sterile water bang the fuck out of it on a tire than inoculate
its what i do
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