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OfflinePlazmotech
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Liquid culture water on tyvek filter?
    #23744771 - 10/17/16 08:11 AM (7 years, 3 months ago)

I have a half pint jar with a lid I made. The lid has a silicone SHIP and a little tyvek filter (the cloth kind from painter's coveralls). The tyvek filter is secured with more silicone.

Because the jar is small, the water is all the way up the top of the jar. When agitating it, I suspect some water hits the lid. However, it's a pretty good moisture barrier, almost hydrophobic I'd say. From the outside, the filter isn't wet or moist at all. Should i be worried at all about contams?


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OfflinecronicrFacebook
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Re: Liquid culture water on tyvek filter? [Re: Plazmotech]
    #23744785 - 10/17/16 08:19 AM (7 years, 3 months ago)

The fact that you have an injection port tells me yes you shold worry about contams.


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InvisiblebodhisattaMDiscordReddit
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Re: Liquid culture water on tyvek filter? [Re: cronicr]
    #23744827 - 10/17/16 08:40 AM (7 years, 3 months ago)

If you're making a LC and you don't already do agar work. You should be really worried about contamination


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InvisibleJosex
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Re: Liquid culture water on tyvek filter? [Re: bodhisatta]
    #23744857 - 10/17/16 08:52 AM (7 years, 3 months ago)

I guess you've been reading some ancient info, or maybe one of those old threads where they tell you to inoculate your honey LC with spores... those are terrible and it's not considered good practice nowadays because of how prone to contamination these kinda LC's are.

Read current info from now on and you'll quickly realize that the last thing a newbie should do is an LC.


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OfflinePlazmotech
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Re: Liquid culture water on tyvek filter? [Re: Josex]
    #23744949 - 10/17/16 09:27 AM (7 years, 3 months ago)

Well, shit. Thanks for the info though. What do I do with a small amount of spores then? Should I do grain instead of PF and just G2G?


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow


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InvisibleMad Season
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Re: Liquid culture water on tyvek filter? [Re: bodhisatta]
    #23744957 - 10/17/16 09:30 AM (7 years, 3 months ago)

Quote:

bodhisatta said:
If you're making a LC and you don't already do agar work. You should be really worried about contamination



Bolded and underlined for your convenience. That's exactly what you should do.


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InvisiblebodhisattaMDiscordReddit
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Re: Liquid culture water on tyvek filter? [Re: Plazmotech]
    #23744958 - 10/17/16 09:30 AM (7 years, 3 months ago)

Absolutely a way better idea. to use spores on PF cakes or grains

Putting spores in a LC is a good way to waste time and spores


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