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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:42 AM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Hey guys relax She was not saying anything about flame-sterilizing inside the SAB, what she said is literally what she said. Of course a torch creates stronger air currents than an alcohol lamp, but everybody uses torches here without issue, just position the torch where it cannot disturb the air around the SAB so much.
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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I haven't even used an SAB yet but, when I used my torch in the bathroom (where I originally did all the ms inoculation) I noticed it was definitely moving air around , that's not good right?
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Edited by Terpfreak (10/17/16 11:58 AM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: DIY alcohol burner [Re: Terpfreak]
#23745387 - 10/17/16 12:00 PM (7 years, 3 months ago) |
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Quote:
Terpfreak said: I haven't even used an SAB yet but, when I used my torch in the bathroom (where I originally did all the ms inoculation) I noticed it was definitely moving air around , that's not good right?
Well, people have got away with open air inoculations in the past, but that's usually done with cakes. A SAB is cheap and easy to build, get one.
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: DIY alcohol burner [Re: Josex]
#23745391 - 10/17/16 12:03 PM (7 years, 3 months ago) |
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I have one one actually, thanks. Just made it yesterday. Starting agar.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: DIY alcohol burner [Re: Terpfreak]
#23745865 - 10/17/16 02:48 PM (7 years, 3 months ago) |
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I use a butane torch in the box with no issues.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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We need a :showmeyourplates: emote
You are needlessly moving air tho :/
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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I don't often get contams on my plates if that's what you're getting at. Here are some recent ones:
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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And why do you use the torch inside the SAB? I'm curious. Surely you concede that you're taking an unnecessary risk, don't you?
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just_curious
Cultivator



Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 6 months
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Re: DIY alcohol burner [Re: Josex]
#23746072 - 10/17/16 03:32 PM (7 years, 3 months ago) |
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I don't see it being anymore of an issue then moving your arms around in the SAB, or swirling your LC around before every inoc.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (06/21/18 11:31 AM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Quote:
just_curious said: I don't see it being anymore of an issue then moving your arms around in the SAB, or swirling your LC around before every inoc.
Do the experiment yourself. Grab some incense sticks, light em up in the sab till there is a lot of smoke and move your arms as you would when doing sterile technique. Observe the movement of the air when you do this and then compare it to the turbulance it creates when you use a torch inside.
I haven't done this myself, but I'm pretty sure the hands will only cause minor turbulance around a small area near your skin, whereas the torch would move all the air in the SAB like clothes in a washing machine.
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just_curious
Cultivator



Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 6 months
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Re: DIY alcohol burner [Re: amidogen]
#23746120 - 10/17/16 03:47 PM (7 years, 3 months ago) |
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It could be a small torch lighter. Same flame, more mobile. I don't use a torch, or put my flame inside if the SAB, but I don't see it making a significant difference in air disturbance compared to what you are already causing when you work.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: DIY alcohol burner [Re: amidogen]
#23746121 - 10/17/16 03:47 PM (7 years, 3 months ago) |
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It's just never been an issue. Period.
Yep, it's a small butane lighter.
Edited by Mycolorado (10/17/16 03:57 PM)
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just_curious
Cultivator



Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 6 months
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Re: DIY alcohol burner [Re: Josex]
#23746129 - 10/17/16 03:49 PM (7 years, 3 months ago) |
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Quote:
Josex said:
Quote:
just_curious said: I don't see it being anymore of an issue then moving your arms around in the SAB, or swirling your LC around before every inoc.
Do the experiment yourself. Grab some incense sticks, light em up in the sab till there is a lot of smoke and move your arms as you would when doing sterile technique. Observ the movement of the air and then compare it to the turbulance it creates when you use a torch inside.
I haven't done it, but I'm pretty sure the hands will only cause minor turbulance around a small area near your skin, whereas the torch would move all the air in the SAB like clothes in a washing machine.
IDK man, not trying to argue. I just don't see it being a huge concern.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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We are not arguing, we're discussing. The whole point of a SAB if still air, that's what'll keep contams at bay. It would only make sense not to stir the air around if you can help it.
Edited by Josex (10/17/16 04:08 PM)
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lvnthalife
Stranger


Registered: 07/20/14
Posts: 662
Loc: ohio river valley
Last seen: 2 years, 11 months
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Re: DIY alcohol burner [Re: Terpfreak]
#23746152 - 10/17/16 03:55 PM (7 years, 3 months ago) |
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Quote:
Terpfreak said: I have one one actually, thanks. Just made it yesterday. Starting agar.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: DIY alcohol burner [Re: Josex]
#23746190 - 10/17/16 04:08 PM (7 years, 3 months ago) |
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Y'all need to get it together
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: DIY alcohol burner [Re: azur]
#23746200 - 10/17/16 04:10 PM (7 years, 3 months ago) |
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Quote:
azur said: Y'all need to get it together

Wow what cracker jack box do that come from
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I got lucky. Girlfriend got it for me for Christmas last year
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