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Offlinesenseit
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Registered: 01/16/16
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How Do They Make (A)PE Syringes?
    #23740968 - 10/15/16 10:03 PM (7 years, 3 months ago)

Recently I obtained some APE and PE Syringes from a trusted vendor. They didn't carry any spore prints for these strains. From what I've read, they don't really drop spores, so people use sterile q-tips to swab the spores from the gills of mature mushrooms.

My question is how do they make (A)PE syringes? Are they immersing the swabs in sterile water or using some other technique to collect the spores?


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Invisibleamidogen
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Registered: 05/07/16
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Re: How Do They Make (A)PE Syringes? [Re: senseit]
    #23740983 - 10/15/16 10:08 PM (7 years, 3 months ago)

.


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Edited by amidogen (04/19/18 11:35 AM)


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OfflineGreg
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Re: How Do They Make (A)PE Syringes? [Re: senseit]
    #23740988 - 10/15/16 10:09 PM (7 years, 3 months ago)

I've read they blend the fruits then separate out the spores in a centrifuge.

I would link to the post I found that information from, but it contains the name of a vendor.


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Invisibleamidogen
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Re: How Do They Make (A)PE Syringes? [Re: Greg]
    #23740989 - 10/15/16 10:10 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (06/21/18 11:33 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: How Do They Make (A)PE Syringes? [Re: amidogen] * 1
    #23741014 - 10/15/16 10:21 PM (7 years, 3 months ago)

https://www.shroomery.org/forums/showflat.php/Number/12190289#12190289

Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.






use that search engine bro


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Offlineblackout
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Re: How Do They Make (A)PE Syringes? [Re: amidogen]
    #23742047 - 10/16/16 10:42 AM (7 years, 3 months ago)

Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.



Quote:

Workman said:
Its an adaptation of an existing method.  I forget where I first read about it but I want to say Mycelium Running by Stamets.  I'd have to confirm that.  The method is self cleaning since the contaminates are lighter than the spores and deposit on the top of the pellet.



Quote:

Workman said:
I left out a step.  You should coarsely filter the mushroom material before centrifuging.  Its probably obvious that you don't need large chunks in the centrifuge.




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Offlinesenseit
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Re: How Do They Make (A)PE Syringes? [Re: blackout]
    #23742446 - 10/16/16 12:48 PM (7 years, 3 months ago)

Very cool thanks for the info. I wasn't asking to create my own, I will just use the swabs and clone fruits for that. It was just out of curiosity.


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Offlinesenseit
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Re: How Do They Make (A)PE Syringes? [Re: bodhisatta]
    #23742470 - 10/16/16 12:56 PM (7 years, 3 months ago)

Quote:

bodhisatta said:
use that search engine bro




What did you search? 'PE Spore syringe method' returns 7 pages of stuff.  Now that I know you use a centrifuge it would have narrowed it down quite a bit. I also didn't look in "Advanced Mycology"


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InvisiblebodhisattaMDiscordReddit
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Re: How Do They Make (A)PE Syringes? [Re: senseit] * 2
    #23742527 - 10/16/16 01:16 PM (7 years, 3 months ago)

https://www.shroomery.org/forums/dosearch.php?where=body&tosearch=both&how=all&words=method+spores+PE&limit=25&forum=c1&showmain=1&expert=1&sort=r&way=d

TC box, only one result per thread, search in mushrooms mycology and psychedelics header(includes cultivation and advanced sub threads)

search query is "PE spores method"

blackout has me on ignore because I call out his bullshit so that's why he replied with the same quote 12 hours later. :lol:


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InvisibleJacobStorm
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Re: How Do They Make (A)PE Syringes? [Re: bodhisatta]
    #23742812 - 10/16/16 02:48 PM (7 years, 3 months ago)

I'm actually working on a method for using a swab to make a  MS syringe. Getting ready to post it up too.


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Offlinesenseit
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Re: How Do They Make (A)PE Syringes? [Re: JacobStorm]
    #23748337 - 10/18/16 09:17 AM (7 years, 3 months ago)

Quote:

bodhisatta said:
TC box, only one result per thread, search in mushrooms mycology and psychedelics header(includes cultivation and advanced sub threads)

search query is "PE spores method"

blackout has me on ignore because I call out his bullshit so that's why he replied with the same quote 12 hours later. :lol:





Cool thanks for the search tips, those are helpful.

Quote:

JacobStorm said:
I'm actually working on a method for using a swab to make a  MS syringe. Getting ready to post it up too.




What do you use the syringes for? Aren't the swabs viable for much longer?


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InvisibleJacobStorm
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Registered: 07/21/15
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Re: How Do They Make (A)PE Syringes? [Re: senseit]
    #23750910 - 10/19/16 12:46 AM (7 years, 3 months ago)

Well sure but I mean in case someone doesn't have access to agar, or has no use for agar, they can make a MS syringe from the swab.


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Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

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azur said: If you like 6th grade results, then 6th grade projects are great.


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OfflineMykoJackson
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Re: How Do They Make (A)PE Syringes? [Re: JacobStorm] * 1
    #26643207 - 05/02/20 08:50 AM (3 years, 8 months ago)

Quote:

JacobStorm said:
I'm actually working on a method for using a swab to make a  MS syringe. Getting ready to post it up too.




Jacob- did you ever dial in this method?  I’d be curious to see what your protocol is.


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