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Re: How Do They Make (A)PE Syringes? [Re: amidogen]
    #23742047 - 10/16/16 10:42 AM (7 years, 3 months ago)

Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.



Quote:

Workman said:
Its an adaptation of an existing method.  I forget where I first read about it but I want to say Mycelium Running by Stamets.  I'd have to confirm that.  The method is self cleaning since the contaminates are lighter than the spores and deposit on the top of the pellet.



Quote:

Workman said:
I left out a step.  You should coarsely filter the mushroom material before centrifuging.  Its probably obvious that you don't need large chunks in the centrifuge.




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