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Offlinenumnum59
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What does this look like?
    #23740905 - 10/15/16 09:37 PM (7 years, 3 months ago)



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Invisibleazur
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Re: What does this look like? [Re: numnum59]
    #23740927 - 10/15/16 09:47 PM (7 years, 3 months ago)

Ba ba bacteria


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblebodhisattaMDiscordReddit
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Re: What does this look like? [Re: azur]
    #23740938 - 10/15/16 09:51 PM (7 years, 3 months ago)

:whathesaid:

grain prep?
what teks you followed
did you follow a tek at all?
sterilization procedure?
what did you inoculate with, etc..


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Offlinenumnum59
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Re: What does this look like? [Re: bodhisatta]
    #23740974 - 10/15/16 10:05 PM (7 years, 3 months ago)

Grain prep doct34s grain prep
Nocd with an lc that ive had positive results with. Im trying to get into agar though.


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OfflineNDStepp84
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Re: What does this look like? [Re: numnum59]
    #23740990 - 10/15/16 10:10 PM (7 years, 3 months ago)

A proper LC is required to be inoculated with agar, then tested on agar.

Doing LC before agar is like trying write a novel before you learn how to read.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Offlinenumnum59
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Re: What does this look like [Re: NDStepp84]
    #23740996 - 10/15/16 10:13 PM (7 years, 3 months ago)

Quote:

NDStepp84 said:
A proper LC is required to be inoculated with agar, then tested on agar.

Doing LC before agar is like trying write a novel before you learn how to read.




Not arguing because i agree but ive been doing ms to lc since i started gowing and havent had a problem in 3 years until i moved into this new house :frown:


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Offlinenumnum59
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Re: What does this look like [Re: numnum59]
    #23741003 - 10/15/16 10:14 PM (7 years, 3 months ago)

Can you point me in the direction on where to start with agar. Im so lost with all the info


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InvisiblebodhisattaMDiscordReddit
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Re: What does this look like [Re: numnum59]
    #23741009 - 10/15/16 10:18 PM (7 years, 3 months ago)

bottom of my signature


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Offlinenumnum59
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Re: What does this look like [Re: bodhisatta]
    #23741023 - 10/15/16 10:25 PM (7 years, 3 months ago)

Missed that, thanks.

What are the chances thats not bacteria?


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Invisibleazur
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Re: What does this look like [Re: numnum59]
    #23741025 - 10/15/16 10:25 PM (7 years, 3 months ago)

:zero:


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Offlinenumnum59
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Registered: 12/07/11
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Re: What does this look like [Re: azur]
    #23741046 - 10/15/16 10:37 PM (7 years, 3 months ago)

This is the second set of jars ive knocked up with this lc. This first is currently in a monotub almost ready to fruit. I pc my syringes before and after use and i switch the needle in between nocs with a new one and still flame sterile that one as well.


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OfflineNDStepp84
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Re: What does this look like [Re: azur]
    #23741068 - 10/15/16 10:46 PM (7 years, 3 months ago)

I'd say you've  had a varying degree bacteria all along in those 3 years, no spore syringe is clean, that jar just happens to be extremely bacterial. Bacterial spawn can produce fruits but BE is affected and weakens myc making it more susceptible to mold.
Creamy looking myc, those wet grains touching the glass, jars that are hard to shake and break up are all some signs of bacteria.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Offlinenumnum59
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Registered: 12/07/11
Posts: 1,189
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Re: What does this look like [Re: NDStepp84]
    #23741102 - 10/15/16 11:05 PM (7 years, 3 months ago)

Quote:

NDStepp84 said:
I'd say you've  had a varying degree bacteria all along in those 3 years, no spore syringe is clean, that jar just happens to be extremely bacterial. Bacterial spawn can produce fruits but BE is affected and weakens myc making it more susceptible to mold.
Creamy looking myc, those wet grains touching the glass, jars that are hard to shake and break up are all some signs of bacteria.




Whats the dead give away that that's bacteria?


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InvisiblebodhisattaMDiscordReddit
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Re: What does this look like [Re: numnum59]
    #23741114 - 10/15/16 11:10 PM (7 years, 3 months ago)

Quote:

Creamy looking myc, those wet grains touching the glass, jars that are hard to shake and break up are all some signs of bacteria.




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Offlinenumnum59
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Re: What does this look like [Re: bodhisatta]
    #23741126 - 10/15/16 11:16 PM (7 years, 3 months ago)

Could it be the grain was too wet when i inoculated?

Somewhat off topic but do you think 25 petri dishes would be enough to get started in agar


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OfflinePortabellaFella 1
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Re: What does this look like [Re: numnum59]
    #23741149 - 10/15/16 11:34 PM (7 years, 3 months ago)

What difference would it make if the grain was too wet? It's still bacterial.

25 dishes won't be enough if you're gonna try to isolate from MS.
Grow it, then clone and clean it using those 25 plates.


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I would like to acquire anything I don’t have


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Invisiblemorty422
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Registered: 07/06/16
Posts: 988
Re: What does this look like [Re: numnum59]
    #23741154 - 10/15/16 11:37 PM (7 years, 3 months ago)

Quote:

numnum59 said:
Could it be the grain was too wet when i inoculated?

Somewhat off topic but do you think 25 petri dishes would be enough to get started in agar




There is no doubt in my mind that the bacteria came from the LC you made. You just can't push a syringe into a LC and expect to have a 100% clean LC. It's up in the realm of winning the lottery imo. :shrug:

25 petri dishes is a good place to start.

If you are only attempting to clean up a drop from a syringe - 25 is more than enough. If you want to start cleaning up clone tissue and/or attempt an isolate. 25 will be ate up rather quickly.

:cheers:


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