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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24235184 - 04/11/17 03:34 PM (6 years, 9 months ago) |
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For some reason I thought LC was LI that you make with nute water and let colonize. Got it now. Thanks for clearing that up for me. Gonna try it when I get off work.
-------------------- KSSS And PE WBS.
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mynakedrat
The phantom hourglass


Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24235209 - 04/11/17 03:42 PM (6 years, 9 months ago) |
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Quote:
Pastywhyte said:
Quote:
Moabfighter said: Trying to wrap my head around the super easy LC.
I still need agar in my LC water? But not enough to make it hard, just to give it some thicker texture than just water?
Anyone got a simple potato flake/honey or Karo recipe?
You make agar and put it in a pint jar with a grain lid. Sterilize.

Then transfer a wedge. Do this in a SAB. Let it grow out a bit to be sure the colony is clean and healthy.

Then add sterile water. Let it colonize. When done pour the colonized water to inoculate.

The super easy part is it works without the need for a ship or whatman filters or syringes.
Wow! I needed that pasty thanks.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Pastywhyte's EzLC tek. [Re: mynakedrat]
#24235444 - 04/11/17 05:26 PM (6 years, 9 months ago) |
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Is pasty plate agar recipe good to use for LC? Trying to stick with what I know or should I add more potato/honey?
Gonna do this in a hour if I can get it good.
-------------------- KSSS And PE WBS.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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The whole mush cult thing doesn't quite click with ya, like you can't connect the dots yet for whatever reason.
Of course dude, that'd work, I haven't done this lc method but it's obvious.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Pastywhyte's EzLC tek. [Re: Josex]
#24235608 - 04/11/17 06:42 PM (6 years, 9 months ago) |
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Quote:
Josex said: The whole mush cult thing doesn't quite click with ya, like you can't connect the dots yet for whatever reason.
Of course dude, that'd work, I haven't done this lc method but it's obvious.
Somewhere along the way, I made a mistake somewhere, and lost ehhhh about 15 grows. I've had two great flushes in about a year. I just like to double check my moves, so I don't keep making bullshit. Sorry if it's aggravating or you don't like to read all my posts or help, I'm just trying to better myself at this hobby. Nothing more, nothing less.
-------------------- KSSS And PE WBS.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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No man, I want to see you succeed, no aggravation taken. So every time you ask such an obvious question I'm like wtf? here we are yet?
Observe more, is all.
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Organic_Magic
Medicine Man



Registered: 10/26/14
Posts: 1,712
Loc: Everywhere
Last seen: 2 years, 6 months
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Re: Pastywhyte's EzLC tek. [Re: Josex]
#24235690 - 04/11/17 07:07 PM (6 years, 9 months ago) |
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Will be trying this with grain water tomorrow in a half pint. good shit pasty, I will post pictures.
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Its all fun and games until mushrooms make you gay
click and learn. Bod's simplified cultivation methods Got side pins?
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Thanks everyone for the help. Giving it a whirl.
Moab's pasty plate recipe LC
-------------------- KSSS And PE WBS.
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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I think it's time to add the water.
-------------------- KSSS And PE WBS.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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So if I'm seeing this right. You transferred a wedge onto some agar that was in the bottom of a media bottle, and will add sterile water to pull nutrients from the agar and grow a nice LC right?
-------------------- JOIN THE POW WOW
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Quote:
TheMadHatter420 said: So if I'm seeing this right. You transferred a wedge onto some agar that was in the bottom of a media bottle, and will add sterile water to pull nutrients from the agar and grow a nice LC right?
Apparently.
Surely it isn't this easy. We'll see....
-------------------- KSSS And PE WBS.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 1 day, 17 hours
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kinda. if your media bottle doesn't have a small neck.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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I had at one point considered this with a QT jar. I was going to ut my lc broth in my media bottle. Then PC it and let cool and attempt to pour the broth into the jar at about 50% colonization, I hope you get success!
-------------------- JOIN THE POW WOW
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Last seen: 1 day, 17 hours
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I just tried LC for the first time. it was a shitshow. I expanded a blue oyster syringe that went alright, but when I tried to tiger drop (wouldn't suggest it) my AA+ plate to 200ml of grain water I made the mistake of using a plate that was way thin on one side and it wouldn't let go. lots of air movement. I also made a transfer that was supposed to be the same as the tek but my dumb ass made the agar way too thick like I'm afraid it would dent a scalpel. next time I'm going to use a small mouth quart pc'd on it's side so it's easy to get a transfer on
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 23 days
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Quote:
tryptkaloids said: I also made a transfer that was supposed to be the same as the tek but my dumb ass made the agar way too thick like I'm afraid it would dent a scalpel.
You can put plates floating/sitting in hot water which will melt the agar or at least have it almost melted and easier to fall away.
It is mentioned a bit, but I think few people do test their agar & techniques etc before sterilizing. i.e. you can boil it briefly and let it set and do lots of practice runs with it in plates, containers, SABs, new scalpels/tools etc. Figure out what is more difficult etc and then go on to properly sterilize it.
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tryptkaloids
Learner



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Posts: 12,641
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Re: Pastywhyte's EzLC tek. [Re: blackout]
#24290931 - 05/02/17 09:34 PM (6 years, 8 months ago) |
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yea my plan is to turn it into more plates. I was just making two small ones and threw way too much in. never really thought about making transfers before sterilization
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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The melting the agar is genius. Hot water bath it, not too hot, but then into SAB, wipe the jar down, carefully open lid and pour water inside, lightly "roll" it, then wait a week or so and inoculate? Can I LC2LC first to keep it going without resetting, or what? Just make a fresh batch with a fresh wedge from a master plate every time?
Thanks.
-------------------- KSSS And PE WBS.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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I think he was talking about letting plates harden before sterilizing and making practice transfers. P.S. you can go lc to lc, I would actually go lc to agar and make lc with that
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Quote:
tryptkaloids said: I think he was talking about letting plates harden before sterilizing and making practice transfers. P.S. you can go lc to lc, I would actually go lc to agar and make lc with that
Ahh I got you. I was thinking my "super ez LC" agar looked quite hard, and I'd basically be pouring sterile water on top of a hard puck. Doesn't sound too ideal. So I thought softening it up a little may help get the nutrients spread about the water.
-------------------- KSSS And PE WBS.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Last seen: 1 day, 17 hours
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what I plan on doing is using grain water (if this actually colonizes.) that way I don't have to rely on the nutes in the agar
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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