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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24141246 - 03/06/17 01:27 PM (6 years, 10 months ago) |
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As promised I have reworked this, added a few more pics, and hopefully explained the advantages better. I have updated the OP and this should now be back on track. Let's make LC with complex nutrition sources and enjoy a sediment free culture.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte] 1
#24141421 - 03/06/17 02:26 PM (6 years, 10 months ago) |
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Super happy this is back!
First time I tried this I didn't have 60cc syringes so I poured water in like in the super easy method
 BRF EZLC

The growth was pretty thick and I had a little trouble with a 16g needle but it tested clean and Ive got almost full colonization in a week from 5cc It also got me thinking about unmodified lids. I have a couple going right now that are unmodified. Hoping that I can still get clean growth and be able to shake the shit out of them to help break up the culture before using my syringe.
Question: Was the grain water LC in your write up made by putting grain soak water on the agar? Or was it in the agar and stained the sterile water?
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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The grain soak water was in the agar. That's why it stayed sediment free. It colored the water but stayed clear. No sediment. You can use nearly anything with this. Brown rice flour which turns the water milky and a poor choice for LC comes out crystal clear this way.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24141476 - 03/06/17 02:50 PM (6 years, 10 months ago) |
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It's weird how the food colouring doesn't leech into the water but grain soak colour will. Either way no sediments
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Actually I have had loads where the food coloring leaches out too. But its never been a problem.
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Edmunter
Mr



Registered: 05/01/13
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24207963 - 03/31/17 11:47 AM (6 years, 9 months ago) |
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Its back ! Nice one!
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,883
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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
#24218178 - 04/04/17 03:17 PM (6 years, 9 months ago) |
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Is there a reason you don't just PC a syringe filled with water inside initially? Or just do the suck-up-boiled-water-4-times method before squirting into the agar dish?
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Pastywhyte
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Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Can't PC a syringe with water, it just boils out. You can do the suck up method but I don't like it myself.
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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24218619 - 04/04/17 05:59 PM (6 years, 9 months ago) |
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Pasty, you care. You care big time, man!
I see you are rocking the grain water recipe.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24218683 - 04/04/17 06:28 PM (6 years, 9 months ago) |
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Quote:
Pastywhyte said: Can't PC a syringe with water, it just boils out. You can do the suck up method but I don't like it myself.
Hope you don't mind me correcting you but you certainly can, I do it all the time without losing a drop of water.
You just have to place them standing up in the pc, needle with needle cap facing upwards, propping them up against a jar or even agar containers.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pastywhyte's EzLC tek. [Re: hamloaf]
#24218699 - 04/04/17 06:35 PM (6 years, 9 months ago) |
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Quote:
Josex said:
Quote:
Pastywhyte said: Can't PC a syringe with water, it just boils out. You can do the suck up method but I don't like it myself.
Hope you don't mind me correcting you but you certainly can, I do it all the time without losing a drop of water.
You just have to place them standing up in the pc, needle with needle cap facing upwards, propping them up against a jar or even agar containers.
Hmmm I will have to give that a try. But now I mostly just pour the water regardless of whether I use a syringe to aspirate at the end.
Quote:
hamloaf said: Pasty, you care. You care big time, man!
I see you are rocking the grain water recipe.
Yes I like the grain water, it's been a staple for years. But lately I get even better recovery with potato/brf recipes. Amazing growth and have seen recovery in 36 hours. Potato/brf done this way produces a crystal clear LC.
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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24218726 - 04/04/17 06:48 PM (6 years, 9 months ago) |
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Yea man, one time I made a LC out 100% rye boil off water, and the myce recovered before it even hit the grains haha.
Nah, but really though, the myce had begun to recover, and expand on the grains THE NEXT DAY!
I was stoked!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pastywhyte's EzLC tek. [Re: hamloaf]
#24218773 - 04/04/17 07:05 PM (6 years, 9 months ago) |
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Yeah the more I try the more I like a complex nutrition profile in my LC.
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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24218938 - 04/04/17 08:22 PM (6 years, 9 months ago) |
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I consider myself a flamboyant LC enthusiast.
It's weird to me though that most folks can't grasp LC'ing.
This is a good write up though, bud. Thanks for it.
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tryptkaloids
Learner



Registered: 02/08/15
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Re: Pastywhyte's EzLC tek. [Re: hamloaf]
#24222758 - 04/06/17 12:15 PM (6 years, 9 months ago) |
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quick question, how do you keep the plastic lids from leaking? even with the plastic ring mine don't seal properly.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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So pouring is good huh. I liked pouring LI and would like to skip the syringe if possible. Gonna look into the aspirating thing.
I plan to use a blender bottle, blender ball, and SFD lid. I can swirl it with the ball in there and it's tall so the filter won't get wet...
-------------------- KSSS And PE WBS.
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tryptkaloids
Learner



Registered: 02/08/15
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why not just use the blender ball in the jar with the filter? I personally wouldn't want to modify my blender bottle
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Wouldn't have to modify anything I don't think.

I could make the LC recipe in unmodified blender bottle, cook, shake. Then in SAB swap the blender bottle lid for a filtered lid when I add my agar plate. Let it colonize. Swirl, not shake it. Sterilize the bottle lid in foil, then swap the lids again in SAB when time to pour.
Would that be correct, incorrect?
Thanks for any help. Hope to try this soon
-------------------- KSSS And PE WBS.
Edited by Moabfighter (04/06/17 12:42 PM)
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tryptkaloids
Learner



Registered: 02/08/15
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I would just pour from the bottle edge, swapping lids can be another vector.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Quote:
tryptkaloids said: quick question, how do you keep the plastic lids from leaking? even with the plastic ring mine don't seal properly.
I simply swirl the liquid to agitate and prevent it from reaching the top of the jar. Works great. I should caution against filling these up too much, increases the risk of leaks and makes pouring harder.
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