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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#23961547 - 12/27/16 11:03 PM (7 years, 1 month ago) |
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About 3 days ago the idea popped into my head to add agar to LC and then I've done some searches... and I came across Jokefox's thread and somehow totally missed this thread and I guess it's too late to see what I missed.
I'm having trouble with LC's lately. For some reason it seems to take a solid week before the culture recovers and visible growth begins. My best guess is that the grains are sucking up the LC like a sponge and dehydrating the mycelium which is preventing rapid recovery. I'm not sure why this would be happening now and in the past I have seen recovery in 24 hours.
But this got me to thinking if I could add some agar to my LC then perhaps the mycelium would retain some water as an agar gel that would coat the grains and prevent the mycelium from becoming dehydrated. I'm trying out my first attempts now.
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Thedillestpickle
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#23961553 - 12/27/16 11:07 PM (7 years, 1 month ago) |
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I'm curious what motivates jokefox and Pastwhyte to add agar to their LC? For me the idea is to maintain the moisture on the surface of the grains for rapid recovery after inoculation. Is anyone else thinking it could be useful for that? and if that's not the goal then what is the idea behind agar in LC?
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Theblingbling
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agar to LC? What?
-------------------- Always up for a trade, pm me ive got lots of peyote seed, prints and chems. Trade list https://www.shroomery.org/forums/showflat.php/Number/23962426
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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well the original post is gone now but I think from reading some of the posts that this was a tek that had agar included in a LC
If you use about 1 gram of agar per 500mL of LC then you'll end up with a nearly liquid solution that has small bits of suspended gel in it. Whether this is advantageous or not I don't know yet, I'm testing it out for myself to see if it helps improve recovery time for inoculated grains.
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Chk
Reverser


Registered: 09/06/13
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#23961670 - 12/28/16 12:23 AM (7 years, 1 month ago) |
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Was really not my point to make you remove your tek Oo that was a cool tek btw..
And if eventually you can remove a step , isn't it even more idiot proof ?
--------------------
Edited by Chk (12/28/16 12:27 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pastywhyte's EzLC tek. [Re: Chk]
#23961686 - 12/28/16 12:37 AM (7 years, 1 month ago) |
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You still don't get it. Obviously the full purpose of the steps are not clear. It will stay deleted until I have time and inclination to rewrite. That way it's full purpose might be clear to even those with no intention of trying it
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Edmunter
Mr



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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#23961784 - 12/28/16 01:58 AM (7 years, 1 month ago) |
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The Tek was simple and Id like to go back to it this month. Is it in your journal? Its quite simple to follow and although ive had 2 failures can see the potential.
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tump
ban the undead



Registered: 03/17/16
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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
#23961795 - 12/28/16 02:06 AM (7 years, 1 month ago) |
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Yes please chill. This method is golden please bring it back
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Pastywhyte's EzLC tek. [Re: tump]
#23962108 - 12/28/16 08:05 AM (7 years, 1 month ago) |
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EDIT: To avoid further confusion
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
Edited by ComebackKid (12/28/16 09:15 AM)
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Edmunter
Mr



Registered: 05/01/13
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Make a nutrient rich very soft agar plate in a small jar, add sterilised water. Use your maths to make 2% LC when water and agar are mixed.
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MrPaulCaruso


Registered: 11/30/16
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Re: Pastywhyte's EzLC tek. [Re: Edmunter] 1
#23967087 - 12/29/16 11:58 PM (7 years, 30 days ago) |
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The higher you fly, the thinner the air gets.
If everything is written for noobs, advanced knowledge ends, and only noobs remain.
Keep doing just exactly what you're doing, Pasty. We need you to keep the bar high.
If you expect everything to be perfect, you will be disappointed. The truest value comes from constantly pushing the envelope to improve art and the science.
When my stepmother goes snow skiing, she NEVER falls. But she never gets any better at skiing, either.
"Take a chance. Get hurt a little. But play as well as you can. Otherwise, you've got nothing to talk about in the locker room." (Maude, from Harold and Maude)
Finally, "illegitimus non carborundum"
-------------------- Tobacco smoke is radioactive. You know -- Chernobyl, Hiroshima, atomic bombs, chest X-rays -- that stuff. Google it.
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 8 days
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24077589 - 02/09/17 02:30 PM (6 years, 11 months ago) |
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Just to clarify, there is nothing actually wrong with the original tek? And the only reason its gone is to avoid confusion?
I'm flabbergasted that people got so mixed up, it was one of the simpler teks around. Colonize agar, add water. Not trying to flame here I'm just surprised.
Hell I'll rewrite it for you if you tell me the finer points I may have forgotten. I enjoy making teks as clear/concise as possible.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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It's gone because the advantages are numerous and clearly its too much for people to understand. It's being rewritten with more pictures and hopefully better explanations.
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Psilosopherr
A psilly goose



Registered: 02/15/12
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24077686 - 02/09/17 03:30 PM (6 years, 11 months ago) |
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glad to hear it. sure sounded good on paper, have yet to try it.
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The_breadsticks
Stranger


Registered: 09/23/16
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte] 1
#24077762 - 02/09/17 04:04 PM (6 years, 11 months ago) |
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Quote:
Pastywhyte said:
I may repost this later once it's more fleshed out and made idiot proof. In its current form it's obviously too complex. Maybe the food coloring is too distracting. Or I might just delete most of my other writeups and stop posting my methods.
Pasty, I really look up to you, and am truly thankful for all the help and info posted.
That said, you're being kind of a baby about this. One idiot not getting it doesn't discount the hundreds of idiots(me) that DID get it. Hope you're having a good day bro, we all love and appreciate your work.
-------------------- FREE TUMP
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Psilosopherr
A psilly goose



Registered: 02/15/12
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shots fired
Edited by Psilosopherr (02/09/17 04:05 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Quote:
The_breadsticks said: That said, you're being kind of a baby about this.
Hey its my method. I could have just kept it to myself and said nothing to no one. I share because I want people to benefit. If I feel that it's not being understood then it's my right to pull it. I don't want any half assed writeups floating around causing confusion.
Seriously having mats floating on top of a LC and thinking that is a reliable method to determine cleanliness is a serious misconception. If something I wrote is responsible for that idea then it needs to go. It's a complete misunderstanding of the method and it's benefits.
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The_breadsticks
Stranger


Registered: 09/23/16
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
#24079315 - 02/10/17 10:37 AM (6 years, 11 months ago) |
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Quote:
Pastywhyte said:
Quote:
The_breadsticks said: That said, you're being kind of a baby about this.
Hey its my method. I could have just kept it to myself and said nothing to no one. I share because I want people to benefit. If I feel that it's not being understood then it's my right to pull it. I don't want any half assed writeups floating around causing confusion.
Seriously having mats floating on top of a LC and thinking that is a reliable method to determine cleanliness is a serious misconception. If something I wrote is responsible for that idea then it needs to go. It's a complete misunderstanding of the method and it's benefits.
It was more so about when you mentioned deleting all your write ups. Come on bro, we don't want to live in a pre-pasty-plate-place.
You know you're a massive part of mushcult and a massive part of a lot of people's success! I am eagerly awaiting the return of this Tek.
-------------------- FREE TUMP
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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I too am curious what I missed.
I don't have any idea what Pasty originally posted, but I wanted to share an experience I had recently. I've been making LC's with agar lately in an effort to improve my results with LC. I was finding my LC wouldn't take off very quickly after hitting grain and tried to come up with a theory as to why. I imagined that the grain was prepped a bit too dry causing it to act like a sponge sucking the moisture out of the LC and hurting the mycelium... just a wild theory, but it led me to try and think of a way to prevent that from happening, so I tried adding agar into the LC. I did some searches and came across an old post that was basically that same idea, and he mentioned how if you got the agar ratio correct you could shake the LC and it would retain bubbles in suspension... thus allowing you to have an aerated LC without the need for a stir-plate.
I found my agar powder didn't perform with the same ratio of agar to water that was prescribed in that thread so I started experimenting with my agar and found that about 1 gram of agar to 500mL produced a consistency that could be easily shaken and still retain bubbles. It seems to take slightly longer to colonize than regular LC, but one interesting advantage is that you can tell that it is clean because if you don't shake it for a few days the mycelium starts to grow along the agar coated walls of the jar. Pros: -you don't need a stir-bar/plate -no stir bar may also mean gentler on the growing mycelium -you can see clean growth on the agar coated walls -even coating of grain without wet-spots -theoretically could have faster recovery time than regular LC -exactly easy as making regular LC once you find the ratio that works for your particular brand of agar
Cons: -You have to get the ratio within a narrow range to obtain the desired effect -If you did want to use a stir-bar you might find it doesn't work well with this method -haven't tested this but there may be issues with storing in refrigerator if the agar gels up too much
I personally think adding agar to LC is an interesting idea, something worth experimenting with. I'd be curious to know if other people have tried it and how they are going about it/what results that have seen and what pros and cons they have determined from their experience.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Okay now I'm going to mention where my method changes.
You don't need to hit a magic number of agar to the formula. You just make agar. You don't worry about it taking a long time to colonize through the heavy fluid. Because you can use anything for the recipe you can have the joy of a brown rice flour and potato flake LC that is crystal clear. See right through it.
Part of why I'm rewriting this with new pics is because I don't think people understood that this lets you get away from soluble media only. These different media types allow for a much faster and robust recovery. So be patient and don't bother to look for this thread to be bumped, I will probably start a new one.
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