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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: Shakedown Street] * 1
    #27124281 - 01/03/21 04:27 PM (3 years, 24 days ago)

IMO LI is full of vectors. I’ve used it and I prefer my methods. Up to you as to what your preference will eventually be. I can talk all day about how something works for me, what you need to decide is what works for you.


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OfflineNodrog99
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27148436 - 01/14/21 07:09 PM (3 years, 13 days ago)

What an incredible TEK.  Thanks so much.

Would it be ok to clone a tissue sample from inside a stem butt onto the agar with this TEK?  I’ve done this with the Pasty No Pour Agar TEK and it worked well.  Hence I assume I can do the same with the EzLC TEK?

Thanks.


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OfflineNodrog99
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Re: Pastywhyte's EzLC tek. [Re: Nodrog99]
    #27149850 - 01/15/21 02:16 PM (3 years, 12 days ago)

Quote:

Nodrog99 said:
What an incredible TEK.  Thanks so much.

Would it be ok to clone a tissue sample from inside a stem butt onto the agar with this TEK?  I’ve done this with the Pasty No Pour Agar TEK and it worked well.  Hence I assume I can do the same with the EzLC TEK?

Thanks.



I appear to have finally found the answer to my own question.  I had read all of this thread but with help of the search facility and correct search criterea I found the following short comment which I had missed earlier:

https://www.shroomery.org/forums/showflat.php/Number/24901927#24901927
(1 PE Biopsy nocced up 12/31.)

So it seems that a biopsy can be used to inoculate the EzLC agar.

However further clarification / confirmation of this would be appreciated.  Regardless I will be having a go at the EzLC tek after I modify the half pint lids today and RTV has set.


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: Nodrog99]
    #27150017 - 01/15/21 04:05 PM (3 years, 12 days ago)

It can be done however, I don’t personally practice that myself. I prefer to be 100% confident in my cultures before I expand them significantly.


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Invisiblemushhead
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27150033 - 01/15/21 04:20 PM (3 years, 12 days ago)

I just wanted to add this here Pasty because I used your method.
Thanks for all the questions you've helped me with Pasty you rock.

PESA EZLC
-> -> -> -> ->
All this was done in an SAB. Syringe/Needle came pre-sterilized.



Here are the jars and the fruiting shoeboxes.

Hats off to you pasty!
:teabird:


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OfflineNodrog99
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27150215 - 01/15/21 05:45 PM (3 years, 12 days ago)

Quote:

Pastywhyte said:
It can be done however, I don’t personally practice that myself. I prefer to be 100% confident in my cultures before I expand them significantly.



Thanks for that Patsy.

Fully appreciate what you’re saying.  As a newb and only interested in producing enough mushrooms for my wife and me (both retired) to eat it’s very small scale.  Plus it’s the experimenting that makes this hobby so exciting for me.  If my cultures fail, contaminate or are lacking it’s not a concern given how little I’m producing.  Just start again and won’t have as many mushrooms to eat for near free occasionally.

Again I want to thank you for your Teks / posts, they really are extraordinary and have been of enormous help.


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OfflineNodrog99
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27150790 - 01/16/21 01:45 AM (3 years, 12 days ago)

Quote:

Pastywhyte said:
I don’t use a stir bar for two reasons, first is that it would just get stuck in the cooled agar puck at the bottom and be limited in its usefulness. ...



I suppose if one wanted to use a magnetic stirrer a sterile stir bar could be added when pouring the water into the jar.  That way it’s not stuck in the agar puck as would be the case if added when PC’ing the agar.  A slight additional risk of contamination but given the lid needs to be removed to pour in the water (unless using the syringe approach) it would be minimal I think? 

Of course as you say the TEK works great without it.  Given that the likes of Mossey Creek Mushrooms have said that a magnetic stirrer does noticeably speed up the process and I have one I will give it a go as an experiment once having mastered the TEK as is to see if it makes any difference.


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OfflineSpectreOfCommunism
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27572991 - 12/07/21 09:58 PM (2 years, 1 month ago)

Sorry if this is a super-nooby question but it was never explained what "aspirating" means in this context. Usually, I understand that word as like breathing something into one's lungs which obviously doesn't fit here -- can someone enlighten me?


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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Offlinenormalperson
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Re: Pastywhyte's EzLC tek. [Re: SpectreOfCommunism]
    #27573118 - 12/08/21 02:45 AM (2 years, 1 month ago)

It means pull LC into the syringe.


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OfflineSpectreOfCommunism
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Re: Pastywhyte's EzLC tek. [Re: normalperson]
    #27574024 - 12/08/21 07:48 PM (2 years, 1 month ago)

Quote:

normalperson said:
It means pull LC into the syringe.



Cool thanks. That was my best guess based on the context but I wanted to make sure I wasn't missing out on some cool esoteric technique


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: SpectreOfCommunism]
    #27574047 - 12/08/21 08:19 PM (2 years, 1 month ago)

You’ll soon learn most of what I do isn’t esoteric whatsoever, most of it is pedestrian bordering on the mundane.


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OfflineSpectreOfCommunism
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27574157 - 12/08/21 10:11 PM (2 years, 1 month ago)

Quote:

Pastywhyte said:
You’ll soon learn most of what I do isn’t esoteric whatsoever, most of it is pedestrian bordering on the mundane.



That's actually what I like so much about your write-ups, Pastywhyte :smile: Just in this case, I didn't get the terminology in question -- thanks again for the great write-up


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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Invisiblettching8475
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27653675 - 02/10/22 11:38 AM (1 year, 11 months ago)

Trying this tek for the first time PW (Super EZ LC style) and a few days in I’m seeing a nice cloud of myc growing out into the liquid media. I’ve been gently swirling the liquid a few times a day and I noticed that the culture is still rooted to the agar surface. Will it release on its own over time or should I expect it to require more vigorous agitation to break it loose/up before pouring it?



--------------------
LAGM 2.022


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: ttching8475]
    #27653801 - 02/10/22 01:30 PM (1 year, 11 months ago)

Give it a few more days, it’s going to leap off into the liquid. Looks good so far.


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Invisiblettching8475
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27653854 - 02/10/22 02:01 PM (1 year, 11 months ago)

:rockon:

Gonna try it with a couple other plates I have coming up as well. Will post results.


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Offlinejjns13k
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #27996584 - 10/13/22 09:16 AM (1 year, 3 months ago)

Just read this whole thread and am definitely going to give it a try. I've got queston that I couldn't answer after reading the whole thing.

When I test the LC after colonization, can I test on another identical jar? Once it shows up clean, use the entire initial jar, and then simply add the water to the 2nd tester jar creating a new LC? Once the 2nd LC is fully colonized, test it on a 3rd etc? Basically just keep these in rotation, using the tester jars as the next generation. Would be way easier than dropping a wedge into the jar each time.


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OfflineHindsight
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Re: Pastywhyte's EzLC tek. [Re: jjns13k]
    #27996589 - 10/13/22 09:24 AM (1 year, 3 months ago)

Yes


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: jjns13k]
    #27996706 - 10/13/22 10:56 AM (1 year, 3 months ago)

Quote:

jjns13k said:
Just read this whole thread and am definitely going to give it a try. I've got queston that I couldn't answer after reading the whole thing.

When I test the LC after colonization, can I test on another identical jar? Once it shows up clean, use the entire initial jar, and then simply add the water to the 2nd tester jar creating a new LC? Once the 2nd LC is fully colonized, test it on a 3rd etc? Basically just keep these in rotation, using the tester jars as the next generation. Would be way easier than dropping a wedge into the jar each time.




Essentially yes. Because each LC starts as a nopour agar you can grow your test into more LC. I’ve done this myself a few times and you can really expand things fast this way.


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Offlinebaldur
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #28050231 - 11/14/22 08:46 AM (1 year, 2 months ago)

I´m planning to use my premixed MEA, and add some malt extract to make it more nutrient rich as the recipe calls for.

Reason being: I live on an island… so would like to use what I can find locally :smile:

The pre mixed agar that I have comes from Beacon Hill Mushrooms, Link to product. Which contains extra light malt extract and agar agar. The recipe on the box calls for 40g per liter of water (which I think contains 20g agar and 20g EMLE). So that gives 2% nutrients and 2% agar.

So the recipe in this thread recommends adding 3-4g of nutrients to 50ml, meaning that the nutrients should be 6-8%.

So let´s say that I´m making 1L of agar for this (in reality I would be making less). I would then mix 40g of the pre mixed MEA powder, and then add 40g-60g of malt extract, giving me 6-8% of nutrients.

Am I thinking this right?


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Re: Pastywhyte's EzLC tek. [Re: baldur]
    #28050300 - 11/14/22 09:26 AM (1 year, 2 months ago)

Yeah you’re on the right track. Don’t sell the potential too short with malt extract tho. Because the agar in this method traps the sediments you can use flours like brf to act as the nute source. Heck even potato flakes work great.


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