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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: hermanium]
    #25290107 - 06/24/18 10:46 PM (5 years, 7 months ago)

Things like not letting the air settle in the SAB or reaching over the jar with your hands.


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OfflineStingingRoger
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #25362959 - 08/02/18 07:46 PM (5 years, 5 months ago)

Really cool way to do LC!

Are whatman filters a necessity when using syringes for bags ? cheers.


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OfflineSpinny
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Re: Pastywhyte's EzLC tek. [Re: StingingRoger]
    #25373678 - 08/07/18 10:29 PM (5 years, 5 months ago)

My question would be using this tek to select a contam free sample. once it is noted that the sample is clean could one just squirt a few cc's on the agar, wait a couple days then withdraw it and then use this liquid to seed a jar of prepared and sterilized grain water jar of say a half pint?

I was thinking that might be better as it would take multiple injections to fill up the jar, and i think i would be more comfortable injecting once instead of opening it up and pouring.

would this work?

thanks.


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: Spinny]
    #25373685 - 08/07/18 10:33 PM (5 years, 5 months ago)

That would totally work fine.


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Offlinemushroomnate
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #25394527 - 08/17/18 01:22 PM (5 years, 5 months ago)

Gonna try this tek with this APE clone.:awesomenod:

Thanks!


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InvisibleJohnny.rotten
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #25415078 - 08/26/18 05:04 PM (5 years, 5 months ago)

going to try this tomorrow, Thanks :takingnotes:


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OfflineNoccer
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #25482500 - 09/23/18 08:39 AM (5 years, 4 months ago)

Looking so forward to trying this, I may even skip the fancy jelly jars so I can start NOW.


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InvisibleBumholio
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Re: Pastywhyte's EzLC tek. [Re: Noccer]
    #25492068 - 09/27/18 02:38 AM (5 years, 4 months ago)

This is most definitely ezaf. Just started an lc yesterday, I wish I'd seen this before then. I may scrap yesterday's and start this today. Thanks pdub:fistbump:


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"great things may come to those who wait, but only what's left by those who hustle"


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Invisible36fuckin5
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Re: Pastywhyte's EzLC tek. [Re: Bumholio]
    #25660413 - 12/05/18 08:48 PM (5 years, 1 month ago)

I tried this out and it seems to have worked wonderfully.

I was pretty loose with it, as usual for me. I did 2 grams agar, 3 grams malt to 100 ml of water. Didn't use it all, but I made about a 3/4" puck in a tall 1/2 pint regular mouth jar with a Whatman filter. Inoculated with an average speed AA+ MS culture.

When it colonized about halfway I just dumped in some sterile water like a G2G. Not sure how much, about an inch from the top.

It's been 9 days and it's definitely the thickest LC I've ever seen. I shake it like a baby I hate every day that I remember. The liquid is crystal clear when I let the myc settle. I haven't tested it yet but it looks amazing. I'll be doing 10 jars with it tomorrow.

Great job, Pasty. This is probably how I'll do LC from now on.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: 36fuckin5]
    #25661324 - 12/06/18 09:58 AM (5 years, 1 month ago)

I’m glad you liked it, I going to be making some up myself today. :rockon:


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Offlinepsillyboy
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #25661817 - 12/06/18 02:16 PM (5 years, 1 month ago)



--------------------
"True terror is to wake up one morning and discover that your high school class is running the country - K. Vonnegut

“The real truth, that dare not speak itself, is that no one is in control. Absolutely no one.” ― Terence McKenna

"LSD is a psychedelic drug which occasionally causes psychotic behavior in people who have never taken it." - Timothy Leary


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Invisible36fuckin5
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Re: Pastywhyte's EzLC tek. [Re: psillyboy]
    #25662325 - 12/06/18 06:16 PM (5 years, 1 month ago)

I hit 10 jars today. We'll see how they do.

I had a thought after I trashed everything though - could you maybe reuse the puck? Mine was awfully thick and I feel like it could have given up a little more. I ended up with about 110 ml of liquid. I got dirty with the last bit and popped the lid off and just sucked it up in the syringe. That syringe wasn't the one I used.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineEdmunter
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Re: Pastywhyte's EzLC tek. [Re: 36fuckin5]
    #25663537 - 12/07/18 11:04 AM (5 years, 1 month ago)

Im trying 3 of these this month.  Do you think this would be good for oyster production?


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OfflineEdmunter
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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #25676021 - 12/13/18 04:25 AM (5 years, 1 month ago)

So Ive already funked out with one of these due to a sticky scalpel but look forward to seeing if I can get this to work this time... 


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OfflineEdmunter
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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #25685333 - 12/17/18 12:49 PM (5 years, 1 month ago)

This one looks clean



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OfflineEdmunter
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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #25695038 - 12/22/18 12:37 AM (5 years, 1 month ago)

So this had a small satalyte (9 o'clock) but it grew as exactly the same mycellium as the central mother.  Adding water in the next few days.



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OfflineEdmunter
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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #25698353 - 12/23/18 01:55 PM (5 years, 1 month ago)


fingers crossed


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Offlinesouthbounpachyderm
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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #25698384 - 12/23/18 02:06 PM (5 years, 1 month ago)

Trying this out today. Thanks for the write up Pasty


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OfflineBobBee
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #25707530 - 12/28/18 11:24 AM (5 years, 1 month ago)

Can you tell me why you do not like to inoculate through polyfil? I am new, and that was my plan for simplicity and hopefully a successful first try


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I am at my happiest when I am with mushrooms.


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Invisible36fuckin5
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Re: Pastywhyte's EzLC tek. [Re: BobBee]
    #25708398 - 12/28/18 07:04 PM (5 years, 1 month ago)

Quote:

Brobry said:
Can you tell me why you do not like to inoculate through polyfil? I am new, and that was my plan for simplicity and hopefully a successful first try




If your Poly is tight enough to be effective it'll bend your needle.

IDK if anyone has tried, but I'm gonna try making a second LC from one of mine. It might have enough left in it. We'll see.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
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