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Offlinetryptkaloids
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Re: Pastywhyte's EzLC tek. [Re: tombosley8]
    #24305177 - 05/08/17 04:32 PM (6 years, 8 months ago)

what is DFA? it's never a bad Idea to change up nutrition sources. I would say pour a kinda thick puck. next time I plan on using a regular mouth quart on it's side to make transferring to it easier.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleJosex
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Re: Pastywhyte's EzLC tek. [Re: tryptkaloids]
    #24305232 - 05/08/17 04:57 PM (6 years, 8 months ago)

Dog food agar.


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: Josex]
    #24305304 - 05/08/17 05:31 PM (6 years, 8 months ago)

I personally prefer to change nute sources.  As for a quart of LC you could use a thicker puck and a more nutritious media. A quart of LC is a fair amount.


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Offlinetombosley8
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #24305412 - 05/08/17 06:21 PM (6 years, 8 months ago)

I did 30 g brf to 12.5 g agar to 625ml water for these 5


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InvisibleBoogieman47
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Re: Pastywhyte's EzLC tek. [Re: tombosley8]
    #24305507 - 05/08/17 06:57 PM (6 years, 8 months ago)

On the bigger jars do you pour in the liquid or syringe it in there??


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: Boogieman47]
    #24305591 - 05/08/17 07:31 PM (6 years, 8 months ago)

I would just pour it myself. In fact I mostly pour and just use syringes to aspirate these days.


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InvisibleBoogieman47
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #24305644 - 05/08/17 07:53 PM (6 years, 8 months ago)

Ya even with a 60cc you would still need like 4 shots and more chances ... i did li to lc on these last jars but i will try this out next time thanks man


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OfflineLennybernadino
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Re: Pastywhyte's EzLC tek. [Re: Moabfighter]
    #24429536 - 06/23/17 03:27 PM (6 years, 7 months ago)

So a quick question about Whatman filters, i looked at the whatman site and found 116 different syringe filters, um which one do we like to use?


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InvisibleBoogieman47
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Re: Pastywhyte's EzLC tek. [Re: Lennybernadino]
    #24429542 - 06/23/17 03:31 PM (6 years, 7 months ago)



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InvisibleGerms
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Re: Pastywhyte's EzLC tek. [Re: Boogieman47]
    #24431231 - 06/24/17 09:36 AM (6 years, 7 months ago)

What's the purpose of the SHIP in the lid? I have some of the blue ones and just got 60cc syringes with 18 guage needles and whatman filters. Do you solely use it to inject water once colonized to size of a quarter, then only use the filter to aspirate into syringe for usage?


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: Germs]
    #24431259 - 06/24/17 09:51 AM (6 years, 7 months ago)

Don't use the filter to aspirate. That's what the SHIP is for. You can use it to inject/aspirate if you are less confident in your ability to pour.


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InvisibleGerms
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #24431290 - 06/24/17 10:06 AM (6 years, 7 months ago)

I just purchased those fancy jelly jars, is pouring an option for those?


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Offlinetryptkaloids
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Re: Pastywhyte's EzLC tek. [Re: Germs]
    #24431327 - 06/24/17 10:29 AM (6 years, 7 months ago)

no pour would be best with those. they are just a little awkward to handle in the SAB


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleGerms
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Re: Pastywhyte's EzLC tek. [Re: tryptkaloids]
    #24431351 - 06/24/17 10:49 AM (6 years, 7 months ago)

It took some more reading but I understand now; the whatman filter isn't actually used to filter the syringe, it's mainly for gas exchange while the SHIP does the injecting and aspirating. I wasn't looking to do pouring anyway as I already have tried that with BLI and while the results are decent I'd like something with quicker growth.

I prefer pouring agar over no pour but for LC this seems very simple


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Offlinetryptkaloids
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Re: Pastywhyte's EzLC tek. [Re: Germs]
    #24431368 - 06/24/17 10:58 AM (6 years, 7 months ago)

even with pouring it should be faster than bli because of the extra nutes and myc growing in there. the main reason I say no pour would be best is because you need to sterilize the jars anyway. petri dishes come sterile. I don't think jars do


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
InvisibleGerms
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Re: Pastywhyte's EzLC tek. [Re: tryptkaloids]
    #24436431 - 06/26/17 11:05 AM (6 years, 6 months ago)

If the whatman filter is for GE why couldn't you just do like a 1/8 inch hole with MP over the top? Just curious as I'm sitting here waiting for the silicone to set so I can throw them in the PC


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's EzLC tek. [Re: Germs]
    #24436955 - 06/26/17 03:29 PM (6 years, 6 months ago)

Micro pore is a poor filter for liquids. But if you read the tek again you will see my alternative options to whatman.


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InvisibleBoogieman47
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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte]
    #24436996 - 06/26/17 03:51 PM (6 years, 6 months ago)

Doesnt the filter allow you to tip and shake without worry??.


Did you see that link i posted pasty? 100 22ug filters for 54$ i know thats us prices but i cant imagine they are much more for you guys


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Offlinetryptkaloids
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Re: Pastywhyte's EzLC tek. [Re: Boogieman47]
    #24437013 - 06/26/17 03:56 PM (6 years, 6 months ago)

some filters can't get wet. period. the filter keeps the jar from forming a vacuum seal and pressurizing the vessel when you extract the water


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
InvisibleBoogieman47
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Registered: 03/05/16
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Re: Pastywhyte's EzLC tek. [Re: tryptkaloids]
    #24437051 - 06/26/17 04:10 PM (6 years, 6 months ago)

I meant those whatman filters sorry i have a bad habit of saying something i know what im talking avout in my head and leaving shit out lmao


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