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InvisiblePastywhyteMDiscord
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Pastywhyte's EzLC tek. * 40
    #23740857 - 10/15/16 09:09 PM (7 years, 3 months ago)

Pastywhyte's EzLC

Like many people I am a closet LC enthusiast. I find it great to inoculate bags and the speed is nice as well. But of course LC is a method that carries high contam vectors and needs a little extra care. With my EzLC we can visually inspect the culture before we introduce the liquid meaning as long as your confident in the liquid phase you can be sure of a clean LC (test it at first to be sure you have your movements etc down). We also are able to make LC as easy as transferring agar wedges, with the same action as blenderless, but an aggressive colonizing culture with plenty of stored energy to recover. I find that with this LC I often see recovery in 48 hours with fast aggressive growth. This EzLC is as simple as making no pour agar and requires little in the way of special equipment. Super EzLC doesn't even require the use of Whatman filters or SHIP's. This is to LC what blenderless LI is to LI.

The best part of the method is the elimination of sediment. You can make brf LC. Or potato flakes. All the sediment is trapped in the agar puck. These more complex media's result in quicker recovery after inoculation. I always found karo or SITR to make for sluggish recovery. I have seen 3 day recovery and explosive growth consistently with some of these recipes you normally would avoid for LC.

Materials needed:

2 half pint or pint jars. Fancy Jelly Jars also work well.
2 LC lid with whatman and SHIP
1 60cc Syringe. Smaller will work but more will be needed.
1 PC

You will also need agar supplies. I like powdered food grade agar and really any nutes that you like works for this. PDA, MEA, GW, DFA are all good and kick ass for this.

So the first thing you need to do is mix up some agar. First you need to decide how much LC you plan to make. 120 ml is a nice amount, more than enough to knock up a pile of grains or bags, and if you do this in widemouth half pints, 120 cc fills it pretty good.

So for 120 ml final LC I want to be using about 2-4 grams worth of nutes, be it potato or malt etc. I then make up about 50 ml of water with 2-4 grams nutrition and 1 gram agar. This puts the agar at 2% and nutrient rich.

I have tailored the above recipe for use of one LC. You will probably want more than that, especially if you plan to use this to test cultures or get bored of growing the same thing all the time.

At the end of the day I wouldn't stress to much over ratios etc. Make up some agar, use lots of nutes, make up the jar.


Quick example:




Bam! Its that damn easy. So now ya pressure cook it up. Once PCd and cool we can now knock these up with a clean culture. Take a clean wedge and put it too the plate same as you would make transfers. Let it grow out to the size of a quarter or so. Do to the high nutes it may grow a touch slower but that's okay cause we don't need it to grow out much.




Once I have a clean culture I can visually inspect it for mold or bacteria or other contams. Once I am happy that its clean I then sterilize a jar of water with a lid outfitted with a SHIP. Have a sterilized syringe ready and then using proper sterile procedure aspirate the water and inject into the culture jar.

Sterile water and syringe:




You will soon notice that the nutes from the agar puck leach into the water (as will the food coloring if you use it) but any sediment or solid matter remain trapped in the agar puck. Give it some time to colonize, usually will take a week to 10 days. Swirling it aggressively will help expand the colony faster. You might dislodge the agar but that is not an issue.

Pic of colonized EzLC




Once colonized you are free to aspirate and use. It makes for a pretty aggressive and fast recovering LC compared to the simple sugar recipes many people make. BRF LC is damn fast to recover and dog food agar usually provides explosive growth. Grain water, potato and of course malt extract all kick ass. It can get very thick if you let it go to long so be careful, I have had it get pretty solid. Makes it hard to aspirate. You can use any size jar for this but I find a quart to end up with more LC than I. Half pints and 120 ml LC is enough to do 60 quarts easy. More than enough. I also like the fancy Jelly jars. They are so low that often you can aspirate the liquid without tilting the jar. Makes things much easier.

Grain water EzLC. Dark but clear.



I may test this one because IME media like grain water or dog food can become susceptible to colonies blooming over time. I would never use grain water or dog food recipes for long term use due to high populations inherent to that media. But it looks very good at first glance.


Super Ez LC

Supposing you want to try this but don't have any LC lids. With taller jars its perfectly safe to use a grain jar lid with SFD and just pour the water and then the LC. You just need to be careful when swirling that you don't get the liquid up to the filter. Taller jar helps with this.

Materials needed

2 Grain jar lids with SFD
2 pint jars (I prefer reg mouth but widemouth can work too)
Agar supplies.

Make your no pour agar the same as with the EzLC. Pour in pint jars and PC. Transfer the wedge as normal but the drop might cause it to bounce off to the side. It doesn't matter if its not dead center, just leave it. When sufficiently colonized sterilize a jar of water and in a SAB or in front of flow hood pour the sterilized water into the colonized no pour jar. As long as the level is kept low (less than 250 ml) its easy to swirl and keep the liquid from hitting the filter.

Potato flakes and karo with simple grain jar lids. Note how thick and healthy the colony looks. These were also mostly ignored, very little agitation was done.



The jars above are crystal clear and look strong. They could be tested if you had any concerns and I recommend testing or simply tossing anything that looks off but, these I personally am confident would be fine.

Once colonized you can simply pour it into your grain jars same as G2G. The colony with this method recovers really fast. Buy a dozen reg mouth pints and keep them rolling real easy. Fast easy spawn. 


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Re: Pastywhyte's EzLC tek. [Re: Hallucyjunkie99] * 2
    #23741180 - 10/15/16 11:57 PM (7 years, 3 months ago)

It could but there would be a lot more vectors. I personally dislike the idea of inoculation through polyfil.

If you don't have SHIPs or whatmans I suggest to do the Super EzLC at the end. All you need is grain jar lids, no ports or syringes required.


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Re: Pastywhyte's EzLC tek. [Re: Pastywhyte] * 2
    #23741183 - 10/16/16 12:01 AM (7 years, 3 months ago)

I'm glad people are interested. The best part of this is the range of media that can become LC nutrition. With traditional LC the media type becomes tricky due to sediments etc. But with this we can get away from the simple sugar I hate, and start working with great media choices. BRF with Karo has delivered insane fast recovery. Dog food is robust. A great way to cut down sediment from grain water.

Hope you like. If it proves bust my next tek is gonna be a home run.


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Re: Pastywhyte's EzLC tek. [Re: Hallucyjunkie99] * 2
    #23741188 - 10/16/16 12:04 AM (7 years, 3 months ago)

I need to flesh that part out with more pics but it is solid. I been using that method a fair amount recently.


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Re: Pastywhyte's EzLC tek. [Re: enlightenment]
    #23742225 - 10/16/16 11:36 AM (7 years, 3 months ago)

Yeah I have used LC over a year old but I never would advise that. The colony at that point is sitting in its own metabolites and losing vigor. Keep your cultures vigorous.

The beauty of this method is that you can toss and start fresh LC very fast. It takes me some time to use a quart of LC and by the end it's losing vigor or it goes in the fridge. Roll with these any you have plenty of culture for the home hobbiests needs and none goes to waste.


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Re: Pastywhyte's EzLC tek. [Re: DrBubbles]
    #23746543 - 10/17/16 05:47 PM (7 years, 3 months ago)

Well ya don't need a marble, it will grow thick and into the liquid media just fine. However I don't like the idea of injecting through the SHIP more than absolutely necessary, by only injecting once it cuts down vectors.

However that said, what you propose will work. I guess at that point it's no different than injecting the myc water into a regular LC. So at that point I'm not sure I would bother with the agar mix. By the way I have started LC that way and it works. Just extra vectors with going through the SHIPs more than needed.


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Re: Pastywhyte's EzLC tek. [Re: stevo]
    #23750242 - 10/18/16 08:46 PM (7 years, 3 months ago)

Honestly what it's gonna come down to at that point is what a person is comfortable with. I have poured liquids, used SHIP's, and cracked lids to aspirate. To be honest my success rate with all those was quite good. All comes down to what a person is comfortable doing. If you have done as much G2G as I have the notion of pouring is pretty easy to get used to.

So if you ain't comfortable with pouring then go with what you are comfortable with :thumbup:


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Re: Pastywhyte's EzLC tek. [Re: Inocuole]
    #23750261 - 10/18/16 08:51 PM (7 years, 3 months ago)

Nice comparison Inoc, covers the pros and cons nicely.


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Re: Pastywhyte's EzLC tek. [Re: tump]
    #23757292 - 10/21/16 08:26 AM (7 years, 3 months ago)

If you are asking if you can do edible species with this the answer is of course. Asian producers use a lot of LC to produce their gourmet mushrooms.

If that's not what you are asking then please rephrase the question.


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Re: Pastywhyte's EzLC tek. [Re: tump]
    #23757536 - 10/21/16 10:03 AM (7 years, 3 months ago)

You could start and expand that way. Just watch the recovery on the agar carefully to be sure the master inoculate was clean. But yes that could work fine, in fact I already did that a few times with no issues.


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Re: Pastywhyte's EzLC tek. [Re: morty422]
    #23784262 - 10/30/16 12:54 AM (7 years, 2 months ago)

Yeah it will scale. I haven't done quarts but I have done pints and it worked the same. I imagine a quart would do fine. Glad you like.


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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #23828635 - 11/13/16 10:46 AM (7 years, 2 months ago)

I usually don't let the edges grow right out. Soon as you can be satisfied that no satellites are going to show and there is no bacteria in the media get the water in.


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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #23845197 - 11/18/16 12:52 PM (7 years, 2 months ago)

In a few days it's going to explode. What recipe did you use for that?


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Re: Pastywhyte's EzLC tek. [Re: enlightenment]
    #23851012 - 11/20/16 10:16 AM (7 years, 2 months ago)

That's definitely bacteria. The agar traps any sediment so if you see anything cloudy I bet it's bacteria. How long did you sterilize for? Dog food isn't like MEA and needs a far longer sterilization cycle. 30 min is not going to cut it with DFA.

Also be very careful of lids that don't seal. I had an LC contam on me once the same way.


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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #23856938 - 11/22/16 10:55 AM (7 years, 2 months ago)

No less than 45 min at 15 psi. I might up that tho cause I had a run where some of my DFA plates went bacterial. Could have been a temp spike that helped it happen but I don't like that being a potential vector.


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Re: Pastywhyte's EzLC tek. [Re: Edmunter]
    #23857100 - 11/22/16 11:53 AM (7 years, 2 months ago)

Depends on the type of dog food and if you use simple sugar in the recipe. My dog food is mostly protein and no grain so when I make it I use grain water or brf to pump it up without risking caramelizing the media.


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Re: Pastywhyte's EzLC tek. [Re: Chk] * 2
    #23957835 - 12/26/16 08:31 AM (7 years, 1 month ago)

Sounds like a pain to me. Aspirating really thick LC is hard to do sometimes, what you propose would be far thicker.

I must ask if you have tried it yet? Hard to improve on something if you haven't tried it. Give one of the methods a shot and see what you think. Personally I don't see pouring the water to be a real vector. It's the same as doing G2G.


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Re: Pastywhyte's EzLC tek. [Re: blackout]
    #23960606 - 12/27/16 01:54 PM (7 years, 1 month ago)

If the agar gells enough to get 2 dimensional growth it's going to be hard to aspirate. If it's not gelling enough to get 2 dimensional growth then it's just plain LC. If you want to make plain LC then do it. This tek is for people who are not looking to make traditional LC.


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Re: Pastywhyte's EzLC tek. [Re: Chk]
    #23960700 - 12/27/16 02:43 PM (7 years, 1 month ago)

Sounds good. I will replace the OP with the links provided :thumbup:


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Re: Pastywhyte's EzLC tek. [Re: ComebackKid]
    #23961451 - 12/27/16 09:57 PM (7 years, 1 month ago)

I am very "for real". If I have written this so poorly that people think it's about adding 0.5% agar to an LC to make a culture form a mat on the surface and that will be enough to spot satellites or bacteria colonies, then I have failed. If a TC can't understand the point of being able to trap media in a puck to allow for sediments to be avoided no matter what media is used, then it needs to be re written, maybe with more pics. If people think it's cool to come into a thread discussing a new method with links to old methods then perhaps it's superfluous to begin with.


I may repost this later once it's more fleshed out and made idiot proof. In its current form it's obviously too complex. Maybe the food coloring is too distracting. Or I might just delete most of my other writeups and stop posting my methods.


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