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OfflineGrefa
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The Trinch Who Stole My Stash
    #23740329 - 10/15/16 04:05 PM (7 years, 3 months ago)

I've been having such a terrible issue with trinch since my grow area got too hot in the middle of the summer, ever since then I'm seeing trinch in ALL of my tubs since then. Now in my latest test to try and avoid this I spawned my tubs in a different part of the house. 1 outa 4 has trinch since I cased. Here is my the setup in explanation. Now prior to this temperature issue which sparked all the trinch I was averaging 4-6 oz dry per 66 qt tubs doing alternating runs of 4 tubs so that 4 are always batching while 4 are fruiting. so 8 tubs a month. 0 contamination issues before my grow area hit 78 deg, with it being too hot inside the tubs to not have an outbreak. THe season has changed, how can I sterilize my area properly to remove this. A friend suggested I turn the heat up to 80, let it run for a week and hose it down with pool shock diluted 50% in a backpack sprayer, and hose it all down. Does anyone have any experience in completely killing this shit? SHould I have some time of ultrasonic humidifier or something to kill all this crap that keeps showing up. Inbetween runs I clean the tubs inside and out with a bleach solution , I go through can upon cans of neutraair, My sterile game is exactly as it was when I was producing well, short of building a flowbox and running that fulltime I'm not sure what else to do.


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InvisibleMad Season
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Re: The Trinch Who Stole My Stash [Re: Grefa]
    #23740429 - 10/15/16 05:01 PM (7 years, 3 months ago)

Golly, that darn trinch!

So are you using agar or what?


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InvisibleTheEaglesGift
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Re: The Trinch Who Stole My Stash [Re: Mad Season]
    #23741277 - 10/16/16 01:11 AM (7 years, 3 months ago)

Quote:

So are you using agar or what?



The million dollar question.


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OfflineGrefa
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Re: The Trinch Who Stole My Stash [Re: TheEaglesGift]
    #23742696 - 10/16/16 02:10 PM (7 years, 3 months ago)

Agar to a master grain jar, then grain to grain


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OfflineGrefa
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Re: The Trinch Who Stole My Stash [Re: Grefa]
    #23742715 - 10/16/16 02:18 PM (7 years, 3 months ago)

And now after having one tub of this batch I went to extremes on sterilization with goto trinch, it looks like another has cobweb, do i just burn my house down at this point and start over?3


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OfflineGet Shwifty
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Re: The Trinch Who Stole My Stash [Re: Grefa]
    #23744294 - 10/17/16 12:29 AM (7 years, 3 months ago)



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InvisibleTheEaglesGift
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Re: The Trinch Who Stole My Stash [Re: Get Shwifty]
    #23744604 - 10/17/16 06:27 AM (7 years, 3 months ago)

Are you saying trinch ironically?


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InvisibleMad Season
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Re: The Trinch Who Stole My Stash [Re: TheEaglesGift]
    #23744951 - 10/17/16 09:28 AM (7 years, 3 months ago)

Pretty sure he is lol. Honestly just sounds like you've been transferring bacteria in every g2g. Was your agar spotless like at the end of this thread?


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OfflineGrefa
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Re: The Trinch Who Stole My Stash [Re: Mad Season]
    #23746473 - 10/17/16 05:19 PM (7 years, 3 months ago)

Here's the process,

Hillbilly Agar (glad rounds) > master jar > 17 qt jars of grain, the 17th is used to spawn the next run, and I usually run the strain like that for 4-8 runs until the yields start to fall off then I start over again. I use 4 qts per 66qt tub, and use Damions bucket tek to prep my substrate.
4qts of water, 650g coir, 2qt verm, handful soluble gypsum. Current room temp 72






Trinch really kills drive for mycology, ive been fighting it and fighting it and lost so many tubs, ive bout reached my limit.


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Edited by Grefa (10/17/16 05:33 PM)


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OfflineTira
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Re: The Trinch Who Stole My Stash [Re: Grefa]
    #23746525 - 10/17/16 05:38 PM (7 years, 3 months ago)

Try proper pasteurization maybe?


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OfflineGrefa
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Re: The Trinch Who Stole My Stash [Re: Tira]
    #23746580 - 10/17/16 05:59 PM (7 years, 3 months ago)

I follow the bucket tek precisely and have not deviated from what has produced for me in the past.  I get water to 180 deg, and seal it in a bucket with my substrate, wait till it cools, and its ready. Same thing I've done time and time again.


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OfflineGrefa
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Re: The Trinch Who Stole My Stash [Re: Grefa]
    #23746589 - 10/17/16 06:01 PM (7 years, 3 months ago)

My initial inquiry in my post was directed at sterilizing work areas and fruiting rooms. Not on my technique. If we could move the post in the direction of that inquiry I'd be grateful.


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InvisibleMad Season
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Re: The Trinch Who Stole My Stash [Re: Grefa]
    #23746659 - 10/17/16 06:17 PM (7 years, 3 months ago)

Let me answer that for you. Why is everyone asking about your techniques?



Are examples of contaminations I had, the 2nd one I had forgotten in my basement for over half a year, with the furnace kicking on every 20 minutes. I had many many contaminations in my days, and I've never felt compelled to clean my room. I got really fuckin anal with agar, as well as spawn, and had these while I was in this situation..



I've never bleached my room. Just a few vacuums. I've never cleaned a few of my FC tubs either. And they had lots of trichoderma..

These bacterial AF trays were fruited in the open air of my basement which had tubs of trichoderma nearby that I forgot about



You spawn in open air, which is covered in contamination spores anyways, fruiting will NEVER be sterile. The thing you need is a clean af established network, that can easily keep contaminations at bay. If you have trich germinating early, it's most likely because bacteria in your spawn has weakened your myceliums "immune system". Bacteria is pretty much always needed for trichoderma to germinate on mycelium.

Weak mycelium contaminates. Kinda obvious really. That means you need stronger mycelium. Which is why people are figuring out your spawn cleanliness, starting with inoculation.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineTira
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Re: The Trinch Who Stole My Stash [Re: Grefa]
    #23746828 - 10/17/16 06:58 PM (7 years, 3 months ago)

Quote:

Grefa said:
I follow the bucket tek precisely and have not deviated from what has produced for me in the past.  I get water to 180 deg, and seal it in a bucket with my substrate, wait till it cools, and its ready. Same thing I've done time and time again.




180 degrees will partially sterilize your substrate, and trust me trich loves that.  I, myself recently realized that bucket tek's just not worth the risk.  It's risky and works only with coir.  Manure gives better results anyway...

Why don't you try giving proper pasteurization a go, and then see how it goes .)


--------------------
Useful Links for Beginners

The Basics              AMU Teks

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Agar for guaranteed spawn,
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InvisibleMad Season
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Re: The Trinch Who Stole My Stash [Re: Tira]
    #23746868 - 10/17/16 07:07 PM (7 years, 3 months ago)

Wait this is manure getting up to 180?? I like never pass 150 with manure. 140-150 for 1 hour. Better to undercook than overcook manure tbh.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineTira
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Re: The Trinch Who Stole My Stash [Re: Mad Season]
    #23749165 - 10/18/16 02:46 PM (7 years, 3 months ago)

Quote:

Mad Season said:
Wait this is manure getting up to 180?? I like never pass 150 with manure. 140-150 for 1 hour. Better to undercook than overcook manure tbh.




He said he's using coir.  But even with coir, if you're having trich problems, proper pasteurization is the way to go, am I right?


--------------------
Useful Links for Beginners

The Basics              AMU Teks

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Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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InvisibleMad Season
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Re: The Trinch Who Stole My Stash [Re: Tira]
    #23749904 - 10/18/16 07:13 PM (7 years, 3 months ago)

Nah. Pasty tosses coir in his PC :lol: coir is fine, and gets packaged sterile at 1000°


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleTheEaglesGift
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Re: The Trinch Who Stole My Stash [Re: Tira]
    #23750472 - 10/18/16 09:57 PM (7 years, 3 months ago)

Quote:

He said he's using coir.  But even with coir, if you're having trich problems, proper pasteurization is the way to go, am I right?




No, you're not right.

Coir doesn't have any microbes to preserve with pasteurization. The reason we pasteurize is to preserve microbes. If they're not present in coir, then what would be the benefit of pasteurizing it?


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OfflineTira
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Re: The Trinch Who Stole My Stash [Re: TheEaglesGift]
    #23752383 - 10/19/16 03:50 PM (7 years, 3 months ago)

Quote:

TheEaglesGift said:
Coir doesn't have any microbes to preserve with pasteurization. The reason we pasteurize is to preserve microbes. If they're not present in coir, then what would be the benefit of pasteurizing it?




That's a good point .)


--------------------
Useful Links for Beginners

The Basics              AMU Teks

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Agar for guaranteed spawn,
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OfflineGrefa
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Re: The Trinch Who Stole My Stash [Re: Tira]
    #23753087 - 10/19/16 08:08 PM (7 years, 3 months ago)

HMMM well maybe I'll try keeping the water at 150. The bucket tek is all I have experience with, as I am sure there are many ways out there to pasterize but I'm dealing with large amounts of substrate, I need a bulk method that works well, on a positive note I do have a 23.5 qt PC. I just think if I went to the extreme of filling jars full of substrate and PCing them would take an eternity.


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Invisibledankington
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Re: The Trinch Who Stole My Stash [Re: Grefa]
    #23753701 - 10/20/16 12:13 AM (7 years, 3 months ago)

The point was more to the fact that coir doesn't house the microbes considered beneficial (the reason for pasteurization).

Pasteurization is of course necessary when using any fecal matter based substrate :p

But coir, is merely the husks from coconuts, and it has never proved to be particularly hospitable in environ. for most bacterium or mold. Nor mycelium, exactly, but its water retention and  It is therefor more noob friendly. Of course, I suppose it could depend.

So, essentially, coir can be sterilized/partially sterilized with little repercussion. That's  why it's so great for city dwelling newbs. Don't hate. Appreciate.


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OfflineGrefa
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Re: The Trinch Who Stole My Stash [Re: dankington]
    #23844945 - 11/18/16 11:08 AM (7 years, 2 months ago)

The entire point of hot water in the verm and coir mix is to hydrate that substrate via steam and heat. The two as themselves aren't normally contamination prone. The main purpose in the bucket tek in my opinion is to hit that 160 degree sweet spot for most things myco related and hydrate the substrate. My issue wasn't in my surrounds or my rooms it was in my LC that I had made from agar. I'd run my agar through several runs, as well as using the LC for several runs. AT some point the LC got trinch, but trinch doesn't show itself in LC, sometimes it won't even be too prevalent in the grain jars, it wasn't until I spawned the tubs that it really show'd its true colors. When in doubt, start over. Seems to be working well for me so far.


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