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Offlinespore-ty
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A dish
    #23739705 - 10/15/16 10:53 AM (7 years, 3 months ago)

Should I let it colonize out and make a master from the whole petri?



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Offlineenlightenment
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Re: A dish [Re: spore-ty]
    #23739709 - 10/15/16 10:55 AM (7 years, 3 months ago)

looks great. :thumbup:

it depends on what your goals are...

you can cut the colonized part out and put it in a master jar right now.


Edited by enlightenment (10/15/16 10:56 AM)


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Offlinespore-ty
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Re: A dish [Re: enlightenment]
    #23739719 - 10/15/16 10:57 AM (7 years, 3 months ago)

To make some masters and expand

How many wedges would you take per master?


Edited by spore-ty (10/15/16 10:58 AM)


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Offlineenlightenment
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Re: A dish [Re: spore-ty]
    #23739737 - 10/15/16 11:06 AM (7 years, 3 months ago)

I like to use about a quarter to a half of a dish per quart jar.
It is just about time. You can be successful by using only a 1mm² piece of agar but it takes some more time to colonize the whole jar.


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Offlinespore-ty
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Re: A dish [Re: enlightenment]
    #23740194 - 10/15/16 03:13 PM (7 years, 3 months ago)

Can i cut into the actual agar and drop that in? Or try to get only myc.?


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Offlineenlightenment
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Re: A dish [Re: spore-ty]
    #23740203 - 10/15/16 03:17 PM (7 years, 3 months ago)



If you want you can cut out some more. But you should only cut colonized agar.

or a nice hexagon:


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Offlinethebug76
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Re: A dish [Re: spore-ty]
    #23740205 - 10/15/16 03:18 PM (7 years, 3 months ago)

Trying to get only mycelium is a pain in the ass. Check out some agar teks on here. The process takes a little practice, but you'll be making clean transfers in no time.


--------------------
Bug 


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Offlineenlightenment
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Re: A dish [Re: thebug76]
    #23740212 - 10/15/16 03:20 PM (7 years, 3 months ago)

cutting a triangle out of colonized agar is a PITA? Why? It takes me 5 seconds :shrug:

//edit

LOL
now i know what you mean.. you just wanted to scrub the agar?
No. Of course you can cut the agar UNDER the myc. But as i said i would not cut the uncolonized parts that are exposed to air.


Edited by enlightenment (10/15/16 03:26 PM)


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Offlinespore-ty
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Re: A dish [Re: thebug76]
    #23740302 - 10/15/16 03:56 PM (7 years, 3 months ago)

Quote:

enlightenment said:
cutting a triangle out of colonized agar is a PITA? Why? It takes me 5 seconds :shrug:

//edit

LOL
now i know what you mean.. you just wanted to scrub the agar?
No. Of course you can cut the agar UNDER the myc. But as i said i would not cut the uncolonized parts that are exposed to air.




Haha ya exactly and ok sweet and man thanks alot for the edited pics really helped  a ton


Quote:

thebug76 said:
Trying to get only mycelium is a pain in the ass. Check out some agar teks on here. The process takes a little practice, but you'll be making clean transfers in no time.





Good shit, any teks in specific you follow?

Now question for both of you guys would yall reccommend a lc?


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Offlineenlightenment
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Re: A dish [Re: spore-ty]
    #23740311 - 10/15/16 03:58 PM (7 years, 3 months ago)

You could transfer the half of the colonized agar to grain and with the other half starting a LC.

You should be careful with LC. Inoculate only one jar to test your LC.


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Offlinespore-ty
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Re: A dish [Re: enlightenment]
    #23740316 - 10/15/16 04:00 PM (7 years, 3 months ago)

I think Ill do that, can you see how may sectors it has or too bad of a picture?


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Offlineenlightenment
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Re: A dish [Re: spore-ty]
    #23740318 - 10/15/16 04:02 PM (7 years, 3 months ago)

It almost looks like an isolate to me. How many transfers did you make?


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Offlinespore-ty
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Re: A dish [Re: enlightenment]
    #23740399 - 10/15/16 04:42 PM (7 years, 3 months ago)

Two lol


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Offlineenlightenment
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Re: A dish [Re: spore-ty]
    #23740410 - 10/15/16 04:51 PM (7 years, 3 months ago)

Nice. I would transfer to grain/LC as i wrote and grow it out. I personally would not make more transfers because you never know how your isolate performs until you fruit/test it. It may look nice on the dish but can produce fruits with low potency and low yield and so on.


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