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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 4 months
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Re: Pasty Agar Tek [Re: Edmunter]
#23732666 - 10/12/16 10:00 PM (7 years, 4 months ago) |
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Has anyone tried stacking these in Qt jars with a lid so they can fit more while having a higher water level in the PC ?
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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I must've goofed somewhere as all I'm growing is bacteriai guess :/
-------------------- KSSS And PE WBS.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Are your blanks bacterial as well?
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Let's pull one out of the PC and see.
Nope. Looks pretty damn blank. All my other plates I inoculate are still blank...
Maybe I just spazzed out on the one bacterial plate. Hopefully that's the case.
-------------------- KSSS And PE WBS.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Could just be a dirty swab or print. It happens, that's why we use agar in the first place. Give em a bit the spores might still germ and be able to to be cleaned up.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 3 months, 18 days
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Re: Pasty Agar Tek [Re: Edmunter]
#23733960 - 10/13/16 12:37 PM (7 years, 4 months ago) |
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Quote:
Edmunter said: Pressure cookers are quicker
maybe for you, not for me & my setup though. I spoke of the timings before, the attentive time for me is minimal as I am confident enough to leave the microwave on when I am not there. I can leave my house after 2-3mins work leaving the microwave on for another 20-30mins or so.
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ghaleon
Stranger
Registered: 10/12/16
Posts: 60
Last seen: 5 years, 6 months
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Re: Pasty Agar Tek [Re: blackout]
#23734510 - 10/13/16 03:27 PM (7 years, 4 months ago) |
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I apologize in advance for all the questions. So far I've had inconsistent success with this. Fortunately, just recently, I purchased a proper pressure cooker (presto 16qt) and am now using a sterlite container as a SAB. Before I was using an electric pressure cooker, and a cardboard box as a SAB. Decided to 'upgrade' due to frustration. I'm sure some of my questions have already been answered in this thread but there's alot to read through and thought it might be easier to just ask.
1) After pressure cooking the agar plate there is still some water sitting on top of the plate. Even after leaving the plate alone in the SAB for some time it never solidifies. What did I do wrong? I brought the pressure up slowly (had the fire on low and then gradually increased over time). Although, admittedly, I was a bit impatient so my idea of 'slowly' isn't enough.
2) For some other plates I would have a little water sitting on top. But then it would solidify after sitting the sab for some time. I believe this is normal/okay/satisfactory?
3) The aluminum foil covering the PP5 container would turn black/burned/charred at the bottom (the part touching the water). Is this something to worry about? Did I overcook? Whenever the PC goes a little over 15 PSI I would just adjust the heat to get the needle as close to 15 PSI as possible.
4) Any recommendations for a good scalpel/exacto knife? The scalpel I bought from amazon couldn't be flame sterilized (only the tip goes orange hot). It was a #11 blade I believe.
Edited by ghaleon (10/13/16 03:30 PM)
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 4 months
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Re: Pasty Agar Tek [Re: ghaleon]
#23734979 - 10/13/16 06:28 PM (7 years, 4 months ago) |
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1-Use more agar
2 & 3-its not a problem
Which scalpel? I like the #8
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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ghaleon
Stranger
Registered: 10/12/16
Posts: 60
Last seen: 5 years, 6 months
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Re: Pasty Agar Tek [Re: Kenetic]
#23735004 - 10/13/16 06:40 PM (7 years, 4 months ago) |
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Quote:
kenetic said: 1-Use more agar
2 & 3-its not a problem
Which scalpel? I like the #8
1- Now that I think about it the problem might be too much water.
4- This is what I bought. It's fucking terrible: https://www.amazon.com/gp/product/B000GKY5UK/ref=oh_aui_detailpage_o00_s00?ie=UTF8&psc=1
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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Re: Pasty Agar Tek [Re: ghaleon]
#23735021 - 10/13/16 06:48 PM (7 years, 4 months ago) |
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ghaleon
Stranger
Registered: 10/12/16
Posts: 60
Last seen: 5 years, 6 months
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Quote:
tombosley8 said: I highly rec this one http://www.ebay.com/itm/NEW-STAINLESS-STEEL-SCALPEL-KNIFE-HANDLE-7-10-SURGICAL-STERILE-BLADES-11-/370795265366?hash=item56551ebd56
The handle never gets too hot and you can torch the whole blade.
Alright thanks. I will try it out.
Edited by ghaleon (10/13/16 07:00 PM)
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Space Coast Slayer
Total Fucking Noob

Registered: 09/19/16
Posts: 158
Loc: I come from the water.
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Damn I had no idea agar could be this simplified.. The couple teks I peeped were pretty intimidating to the average noob but this seems real beginner friendly.. Thanks for taking the time to share this..
-------------------- Everything I post is pure fiction and all images were stolen from google.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 12 days
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Re: Pasty Agar Tek [Re: ghaleon]
#23736214 - 10/14/16 07:13 AM (7 years, 4 months ago) |
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Quote:
ghaleon said: I apologize in advance for all the questions. So far I've had inconsistent success with this. Fortunately, just recently, I purchased a proper pressure cooker (presto 16qt) and am now using a sterlite container as a SAB. Before I was using an electric pressure cooker, and a cardboard box as a SAB. Decided to 'upgrade' due to frustration. I'm sure some of my questions have already been answered in this thread but there's alot to read through and thought it might be easier to just ask.
1) After pressure cooking the agar plate there is still some water sitting on top of the plate. Even after leaving the plate alone in the SAB for some time it never solidifies. What did I do wrong? I brought the pressure up slowly (had the fire on low and then gradually increased over time). Although, admittedly, I was a bit impatient so my idea of 'slowly' isn't enough.
2) For some other plates I would have a little water sitting on top. But then it would solidify after sitting the sab for some time. I believe this is normal/okay/satisfactory?
3) The aluminum foil covering the PP5 container would turn black/burned/charred at the bottom (the part touching the water). Is this something to worry about? Did I overcook? Whenever the PC goes a little over 15 PSI I would just adjust the heat to get the needle as close to 15 PSI as possible.
4) Any recommendations for a good scalpel/exacto knife? The scalpel I bought from amazon couldn't be flame sterilized (only the tip goes orange hot). It was a #11 blade I believe.
1) try this 22) Potato dextrose yeast agar: 10g agar, 10g potato starch, 7g dextrose, 1g yeast 2) You can pour the water out in the SAB by opening the lid a tiny amunt upside down
3) Standard
4) any metal scalpel that you can flame will work. If you flame it and it doesnt turn red, it doesnt mean it isnt sterile. Its the heat of the torch that burns all the contamination of the blade. If you put any metal blade under intense heat for 5-10 seconds its sterile.
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7munkee
Berilion



Registered: 08/10/15
Posts: 273
Loc: North east US
Last seen: 16 days, 17 hours
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Re: Pasty Agar Tek [Re: DmTree]
#23736326 - 10/14/16 08:29 AM (7 years, 4 months ago) |
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The microwave heats too unevenly for my taste. I have never had any luck with it.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 hours, 10 seconds
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Re: Pasty Agar Tek [Re: 7munkee]
#23737458 - 10/14/16 03:09 PM (7 years, 4 months ago) |
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it doesn't need to get completely red. aslong as it sizzles when cooled then you're fine
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Maeltne
Stranger
Registered: 08/05/16
Posts: 9
Last seen: 7 years, 4 months
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I realize a PC is ideal; but how might this be adapted to boiling (or microwaving) sterilization?
Edited by Maeltne (10/15/16 04:24 PM)
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 15 days
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Re: Pasty Agar Tek [Re: Maeltne]
#23740373 - 10/15/16 04:28 PM (7 years, 4 months ago) |
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Boiling or microwaving is not an option. It is possible with a high contam rate but who wants this? Ebay -> pressure cooker. It is worth the money.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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agar can be sterilized without pressure, steam sterilize agar for 60m at least. also do not use grain soak water or home made PDA. use "lab grade" media that dissolves completely leaving a clear transparent solution with no sediment or turbidity/opaque. it's not ideal but you can get away steam sterilizing some media that way.
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ghaleon
Stranger
Registered: 10/12/16
Posts: 60
Last seen: 5 years, 6 months
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Alright guys, I'm a bit worried here. I just pressure cooked some new plates but I don't think I put enough water in here. I'm using the presto 16-quart cooker and put 1.5 quarts of water in there (which is the minimum). Whenever I put more the pasty plates float in the water and aren't still. When I was done pressure cooking for 45 minutes there was still a little bit of water left (the water level was under the rack).
1) Do the pasty plates need to be in direct contact with water for the entire duration of the 45 minutes to be properly sterilized/cooked? I suspect it was only properly cooked for 20 or so minutes before too much water getting evaporated. Leaving the plates getting cooked above water.
2) Was I supposed to be 2 or more quarts of water in there? Won't that increase the chances of the plates tipping over and being cooked on its sides?
I'm panicking a bit here. I won't be able to use the pressure cooker again until Thursday. My roommates HATE the sound it makes when it's on 15 psi.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pasty Agar Tek [Re: ghaleon]
#23746456 - 10/17/16 05:16 PM (7 years, 4 months ago) |
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Use jar rings to elevate them if the rack isn't high enough, you don't want them in contact with the water. A few inches is enough, I only use an inch worth myself. It should be plenty for the run.
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