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Offlinetombosley8
Full on... Bossley Baggins
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Registered: 10/14/13
Posts: 3,660
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Re: Fresh air exchange best way to achieve this? [Re: PortabellaFella 1]
    #23734351 - 10/13/16 02:39 PM (7 years, 3 months ago)

ok this thread is way bad. no offense op. :facepalm:

For real check out All of spitball jedi's stuff and stick to the teks.

MAKE A SAB. the glove box attached to a hepa filter is going to cause a lot of turbulence making the outside air(billions of contams) able to be mixed in and blow all around your sterile work.  the still air box will stop all movement of air and will trap the contams present with the soapy water you mist in the box...

WBS SHOULD NOT BE USED AS CAKES.  well it can be but with poor results even when done properly with a casing layer in a proper monotub setup.

USE WBS AS SPAWN FOR BULK SUBSTRATES. once your wbs is fully colonized you break it up and mix it with a bulk sub (such as coir, vermiculite, and gypsum) in open air and it will colonize just fine as long as the spawn was 100% clean. 

which leads me to the next part.

TO GET CLEAN SPAWN YOU WILL NEED AGAR. When doing the pf tek you can have contammed cakes from dirty syringes and still get mediocre results because the brf mix is resilient when left in cake form. When using whole grains you want 100% clean or else your results will diminish greatly and you will usually end up with mold further contaminating the environment.

NOCCING UP WBS AND ANY OTHER GRAIN IS A MAJOR NO NO! No spores are clean whether from print/swab/syringe unless printed in a sterile environment which is very rare and yours definitely were not.

DO a lot more reading and AS I SAID< FOLLOW SPITBALL JEDI'S STUFf AND ALL THE TEKS HE LINKS TO> will save you a lot of time/research/question asking.

Spitball Jedi's stuff

BEST PLACE TO START AGAR< PASTY PLATES

READ THIS TOO
Quote:

PortabellaFella 1 said:
Just look in bodhisatta's sig for the link that says where to begin. Start there. Read on how to do it right. Using the search function works as well but can be overwhelming with so many results. Look at what veterans and trusted cultivators do/suggest and follow them to a T.

Ditch the glove box with air blowing through a hepa and make a still air box. No blowing air whatsoever unless you have a proven flow hood. Shut off fans and a/c units. Spray the room liberally with Lysol or oust to help keep contaminations at bay.

Proper procedure and cleanliness work well with pf tek.




:whathesaid:  EXCEPT YOU REALLY DON'T NEED TO GO CRAZY WITH THE LYSOL AND OUST CRAP. Water misted in the air almost works just as well. I stopped using that crap unless i'm doing a deep clean on my bathroom spawn room or something similar.

Just keep the air STILL and the general area dusted and wiped and you should be fine.

Also portafella I think it was wbs we are talking about?:shrug:


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Edited by tombosley8 (10/13/16 04:34 PM)


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
Loc: where?
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OG Cultivator
Re: Fresh air exchange best way to achieve this? [Re: JuliaD]
    #23734381 - 10/13/16 02:48 PM (7 years, 3 months ago)

Quote:

JuliaD said:
So what's a good air exchange tek for monotubs?




frank makes it easy.

and spitballjedi teks are even easier.


Edited by mushboy (10/13/16 02:49 PM)


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OfflinePortabellaFella 1
Enthusiastic
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Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
Re: Fresh air exchange best way to achieve this? [Re: mushboy]
    #23734432 - 10/13/16 03:04 PM (7 years, 3 months ago)

@tombosley8. Yeah man, I asked for full procedure and then didn't read the full procedure lol


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I would like to acquire anything I don’t have


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Fresh air exchange best way to achieve this? [Re: PortabellaFella 1]
    #23735421 - 10/13/16 09:05 PM (7 years, 3 months ago)



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