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OfflineJohnnyCakes
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Registered: 08/29/16
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Last seen: 6 years, 2 months
Burnt bacon smell in incubator *DELETED*
    #23729089 - 10/11/16 06:33 PM (7 years, 3 months ago)

Post deleted by JohnnyCakes

Reason for deletion: I answered my own question. A rash posting



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OfflineJohnnyCakes
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Re: Burnt bacon smell in incubator [Re: JohnnyCakes]
    #23729415 - 10/11/16 08:28 PM (7 years, 3 months ago)

Welp, the smell was obviously contamination, and I should have been able to tell that even as a noobie. I apologize for posting when I knew the procedures. I looked at both my bags to compare, and one had growth in it. A lot of wet growth. Does anyone know what this stuff is? Just curious now, I threw it out.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Burnt bacon smell in incubator [Re: JohnnyCakes]
    #23731860 - 10/12/16 05:00 PM (7 years, 3 months ago)

Bacteria, but I'm not positive what kind.

I personally wouldn't bother incubating unless I couldn't get my room above 68ish f. They can be a fire hazard at worst, or a waste of electricity at best. Though if your temps cause you really need it, then by all means use it. Just make sure to be safe.

What temp did you have the incubator set to?


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OfflineJohnnyCakes
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Re: Burnt bacon smell in incubator [Re: TheEaglesGift]
    #23732582 - 10/12/16 09:29 PM (7 years, 3 months ago)

Sorry for being all over the place with this posting, answering my own questions and deleting stuff.
My incubators have been averaging between 70-75 the past week but were at 75-80 the week before. Today I re-checked the incubator and found the other grain bag to be contaminated as well, it reeked of the same smell. It had white growth which looked healthy but also wetness around grains on the other side of the bag.
So I had to toss out both of my bags in the end. I am pretty sure I messed up by not wiping the inject ports with alcohol before inoculating the bags in the oven.
is my incubator safe? it still smells kinda like burnt bacon and I have 6 jars in there


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InvisibleThe Phleg
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Re: Burnt bacon smell in incubator [Re: JohnnyCakes]
    #23732599 - 10/12/16 09:34 PM (7 years, 3 months ago)

isolate that bacon smell, it might be worth fortunes!


--------------------
You wanna get high? Drink tap water.
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InvisibleTheEaglesGift
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Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Burnt bacon smell in incubator [Re: The Phleg]
    #23732677 - 10/12/16 10:05 PM (7 years, 3 months ago)

Why and how are you inoculating your bags in the oven?


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OfflineJohnnyCakes
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Re: Burnt bacon smell in incubator [Re: TheEaglesGift]
    #23734763 - 10/13/16 05:04 PM (7 years, 3 months ago)

I don't have an SAB or a flow hood or any other sterilized space, so I figured why not inoculate the bags in the oven?
I just wiped the needle and injected it in the "sterile" space of the oven like I did with my jars


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OfflineOfTheVoid46
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Re: Burnt bacon smell in incubator [Re: JohnnyCakes]
    #23734806 - 10/13/16 05:22 PM (7 years, 3 months ago)

Take a big cardboard box and cut off the top and cut two holes in the side.

Put saran/plastic wrap all over the top and tape it.

Do the same for the two arm holes.

When it comes time to use it simply poke through the plastic covering the arm holes which will somewhat seal them around you.

It's ghetto and far from perfect but I've done it in times of need and I've never had a problem with it.

It's definitely not my go-to but it's better than nothing.

EDIT: Obviously put everything in it before you tape it up. Myself, I spray everything with disinfectant as I put it in and spray down the inside of the box too but that's just me.


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Edited by OfTheVoid46 (10/13/16 05:23 PM)


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OfflineJohnnyCakes
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Re: Burnt bacon smell in incubator [Re: OfTheVoid46]
    #23735652 - 10/13/16 10:36 PM (7 years, 3 months ago)

I read about that technique. Does a cardboard box as a glove box work alright for a highly contaminated zone? My house is very difficult to keep sterile, as the kitchen, bedroom, and bathroom are all connected. I want to work with agar as well and know the oven tek wouldn't work for that...
Edit: I am worried about the arm holes mostly. Will that actually work? Does not seem like a true SAB


Edited by JohnnyCakes (10/13/16 10:43 PM)


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InvisibleTheEaglesGift
The Nagual


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Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Burnt bacon smell in incubator [Re: JohnnyCakes]
    #23735956 - 10/14/16 02:46 AM (7 years, 3 months ago)

Just build a still air box. They're simple and cheap to make. Find a big tote, bigger the better since you don't want to be cramped.

If making a SAB is too hard or expensive for you then you're in the wrong hobby. Inoculating in the stove isn't wise.


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OfflineJohnnyCakes
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Re: Burnt bacon smell in incubator [Re: TheEaglesGift]
    #23737653 - 10/14/16 04:20 PM (7 years, 3 months ago)

Got a big box and made a cheap one. I will try this out and see how it compares with the oven.
I inoculated all my jars with the windows open, my porch door open, and my air filter running, in the oven, and they are still uncontaminated as far as I can tell. Just the bags got contams. I don't think this glove box would do nearly as well in the same environment. So, it's just a matter of convenience with whether or not I have still-air, and what kind of work it is. For example, with agar I will close all off air flow and use this glove box. The oven tek has proven itself surprisingly successful to me though for the PF tek.
I appreciate all the advice:thumbup:


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InvisibleTheEaglesGift
The Nagual


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Re: Burnt bacon smell in incubator [Re: JohnnyCakes]
    #23738933 - 10/15/16 12:30 AM (7 years, 3 months ago)

Are you running the oven while you do this?

What is the value exactly of inoculating in your oven? What is the gain of it?


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OfflineGet Shwifty
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Re: Burnt bacon smell in incubator [Re: TheEaglesGift]
    #23739815 - 10/15/16 11:43 AM (7 years, 3 months ago)

Quote:

TheEaglesGift said:

What is the value exactly of inoculating in your oven? What is the gain of it?




The heat floats away all of the airborne contams from your jar and syringe. It has worked for me more often than not.


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OfflineJohnnyCakes
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Re: Burnt bacon smell in incubator [Re: Get Shwifty]
    #23739996 - 10/15/16 01:48 PM (7 years, 3 months ago)

I apologize for never explaining that. Here's the breakdown: I let the oven bake at 325 for 20 minutes to sterilize and then I let it sit at 225 for 10 minutes and then began the inoculation on a tray pulled out of the oven. I used a cardboard box as the base for temporarily setting things down and sprayed the entire area with Lysol before beginning. I worked one jar at a time, and wiped my needle with alcohol between each one. The oven functions as a sort of household flow-hood, but not really. Get Shwifty sort of got it. I think it more floats contams away from the injection area, just outside the oven. The heat does not get rid of contamination on your jars or needle! Both need to be properly sterilized before beforehand as standard before inoculating in a sterile area, just like with a SAB.

I did not realize it does not work as well with the unforgiving grain substrates. Thus far I can only approve of its use for injecting jars with spore wata. It has worked even in incredibly unsterile, moving air environment. so, to answer your question, there is much to gain if you cannot access any sort of still air or sterilized air and still want to start your inoculation process with the PF tek.


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InvisibleTheEaglesGift
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Re: Burnt bacon smell in incubator [Re: JohnnyCakes]
    #23741066 - 10/15/16 10:45 PM (7 years, 3 months ago)

I'll stick to still air. It's easy to produce. All it takes is a box.


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Burnt bacon smell in incubator [Re: JohnnyCakes]
    #23744080 - 10/16/16 10:20 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 11:39 AM)


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OfflineGet Shwifty
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Re: Burnt bacon smell in incubator [Re: amidogen]
    #23746149 - 10/17/16 03:54 PM (7 years, 3 months ago)

Quote:

amidogen said:
Quote:

Get Shwifty said:
Quote:

TheEaglesGift said:

What is the value exactly of inoculating in your oven? What is the gain of it?




The heat floats away all of the airborne contams from your jar and syringe. It has worked for me more often than not.



:facepalm:

Quote:

JohnnyCakes said:
I apologize for never explaining that. Here's the breakdown: I let the oven bake at 325 for 20 minutes to sterilize and then I let it sit at 225 for 10 minutes and then began the inoculation on a tray pulled out of the oven. I used a cardboard box as the base for temporarily setting things down and sprayed the entire area with Lysol before beginning. I worked one jar at a time, and wiped my needle with alcohol between each one. The oven functions as a sort of household flow-hood, but not really. Get Shwifty sort of got it. I think it more floats contams away from the injection area, just outside the oven. The heat does not get rid of contamination on your jars or needle! Both need to be properly sterilized before beforehand as standard before inoculating in a sterile area, just like with a SAB.

I did not realize it does not work as well with the unforgiving grain substrates. Thus far I can only approve of its use for injecting jars with spore wata. It has worked even in incredibly unsterile, moving air environment. so, to answer your question, there is much to gain if you cannot access any sort of still air or sterilized air and still want to start your inoculation process with the PF tek.



:facepalm: :facepalm: :facepalm:

Both of you. Please never give this advice to anyone. Ever.

At least you've made the leap in logic that since the hot air isn't killing you, it's also not killing the millions of spores floating around your kitchen.

The leap you've failed to make is that having your shit right outside your hot oven that is open to your much less hot kitchen is creating a fuck ton of air currents. Guess what rides all over and onto everything with those currents? Contaminate spores of every variety.

How do you not see how terrible of an idea this is compared to an SAB? I think it's because you think SAB stands for Sterile Air Box :facepalm:

There is no barrier to owning an SAB. Anything you can come up with is bullshit.

You buy a large clear tote for $5 at most, a cheap spray bottle for $1.50, and a bottle of soap for no more than that. You cut two arm sized holes in tote, and wa fucking la. SAB. You fill your spray bottle with a drop of soap, then add water. You put anything you want to work on in it, spray down the sides with soapy water, then spray into the air above the SAB. Close the lid, wait 5 minutes for the air to settle. Flame sterilize any instruments you use with a torch, sanitize hands, jars, etc with 70 iso. Move slowly. Contaminates that were in the air before you sprayed stick to the wet soapy box sides, and by keeping movements slow, any you stir up end up there too. Still Air Box. Still. Not sterile.

There is no good reason to not have an SAB, and your luck with the oven method is not proof that it's a viable alternative to an SAB. There is no need for "sterile air". You can manage non-sterile air effectively with an SAB, not with a fucking oven. Cook pizza with your oven. Don't inoculate with it. Please never say those things again, ever. Do a little research before you make a public service announcement that some noob will see, follow, and then end up on the contam forums like you. :micdrop:

But don't take my word for it. If you really want to see just how ineffective your oven method is, try successfully knocking up an agar plate in front of it. Agar is brutal when it comes to showing you every vector you expose it to. Try it with your oven, then again with the $7 of money and 10 minute of time to set up your SAB and do the same.

:facepalm:




I was only passing on info that was given to me from a major growkit supplier. Do you want to send them some angry emails?

Try being less snippy with people when correcting them, its a life skill.

With that said, I will no longer offer "advice" of any kind.


Edited by Get Shwifty (10/17/16 04:00 PM)


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Invisibleamidogen
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Registered: 05/07/16
Posts: 1,782
Re: Burnt bacon smell in incubator [Re: Get Shwifty]
    #23746158 - 10/17/16 03:59 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 11:46 AM)


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OfflineGet Shwifty
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Registered: 10/14/15
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Re: Burnt bacon smell in incubator [Re: amidogen]
    #23746165 - 10/17/16 04:01 PM (7 years, 3 months ago)

You know, for people who trip a lot some of you seem awfully aggressive.


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Burnt bacon smell in incubator [Re: Get Shwifty]
    #23746196 - 10/17/16 04:09 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 11:46 AM)


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