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Offlinespore-ty
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Registered: 01/21/16
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23730012 - 10/12/16 12:07 AM (7 years, 3 months ago)

Quote:

mrknowitall95 said:
I thought LC was almost impossible to tell if its contaminated? What would be the benefit of MS to LC over MS to agar, clean it up, then turn any one of the clean cultures into however much LC you need? Have you ever had any contaminated LCs?

I should have only done agar but I was eager to start grains so I took a risk. As long as you guys say the plates could possibly still grow I will keep watching them!





Do this, spore to lc isnt a safe route


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InvisibleComebackKid
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23730267 - 10/12/16 05:50 AM (7 years, 3 months ago)

Quote:

mrknowitall95 said:
Good to know. Why is it that brf cakes are usually ok with spore syringes then?



If you asked me a month ago I would have an answer for you. Unfortunately I forget but I have a feeling its because brf is nutrient rich?
Brf cakes are usually bacterial as fuck though dont be fooled.

Quote:

mrknowitall95 said:
I thought LC was almost impossible to tell if its contaminated? What would be the benefit of MS to LC over MS to agar, clean it up, then turn any one of the clean cultures into however much LC you need?




Youre absolutely right. Dont fuck with LC untill after you have some clean cultures on agar... so much crap advice in one thread :facepalm3:

EDIT: Also please dont go putting h2o2 in your agar plates. Peroxide has no business being in there!


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Edited by ComebackKid (10/12/16 05:54 AM)


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OfflineMortySmith
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Registered: 03/28/16
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Last seen: 6 years, 4 months
Re: Started grains and agar. Neither are going too well, advice needed. [Re: ComebackKid]
    #23730298 - 10/12/16 06:23 AM (7 years, 3 months ago)

Funny, when I was growing my BRF cakes after the first flush, I even thought to myself "Yeah, I mean I guess the mycelium is still pretty healthy, but it just seems a bit off in some places." But I didn't really know, because it was my first experience with mycelium at all.

Then after the second flush while more carefully examining the cakes I was like "Oh yeah, these are bacterial." It wasn't super dark green or pink or anything but you could just tell that the mycelium was having a hard time fighting something instead of just growing more mushrooms.


Edited by MortySmith (10/12/16 06:32 AM)


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OfflineJesusDaMartian
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Registered: 04/27/14
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23730484 - 10/12/16 08:30 AM (7 years, 3 months ago)

*Inoculate form syringe to grain .
*Flame Sterilize Needle
*Use pillow stuffing as the filter
*Do inoculation in a still air environment
*Don't forget to sterilize the grain 90 min at 15 psi
-Also make sure to add gypsum when soaking- It makes the grain easy to shake. Easy shake = Easy colonization .
Good luck friend


--------------------
"If you smile at me, I will understand
'Cause that is something everybody everywhere does
In the same language"
-Wooden Ships


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OfflineMadSeasonStudent
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23730837 - 10/12/16 10:50 AM (7 years, 3 months ago)

Looks super wispy to me. Have you shook the jar yet?
10 days is really a long time and it should be colonized imo
And since they arent, probably a few reasons why..molds, bacteria etc.
Takes 3 transfers to clean a plate up according to RR

So with that in mind. Start a few plates for agar control and transfer and you will be ahead of the game with clean spawn. Those pp5 containers work slick for agar. 10 for 2 at dollar store.


--------------------



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InvisibleMad Season
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Registered: 09/16/12
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MadSeasonStudent]
    #23730917 - 10/12/16 11:14 AM (7 years, 3 months ago)

That isn't cobweb (dactylium) that is some grey ass mold that wishes it could colonize as quickly as cobweb.

Oh and 6 days is nothing for germination on agar. In fact if it germinated quicker, often times it's some green ass mold that germinates quicker.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinesporeworker
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Registered: 07/21/16
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Last seen: 6 years, 1 month
Re: Started grains and agar. Neither are going too well, advice needed. [Re: Mad Season]
    #23731178 - 10/12/16 12:51 PM (7 years, 3 months ago)

Quote:

I thought LC was almost impossible to tell if its contaminated? What would be the benefit of MS to LC over MS to agar, clean it up, then turn any one of the clean cultures into however much LC you need? Have you ever had any contaminated LCs?



  yes it is harder to tell if contamination is present, however the reason i suggested this is lc grows allot faster than ms saving time (since colonization time is so short i just test a sample before using a stored lc).  yes that was line of thinking, a lc can be used for agar & visa versa so you could either make agar samples until your satisfied or expand that into a lc (preference i suppose.. ima fan of lc inoculating while some love strictly working with agar).  out of about quite a few lc jars iv only experienced 2 contams. back when i was shaking jars, pretty sure i got filter disc wet.. made a stir plate & started using stir bars and iv had great luck:cool:  ...as i said above i suggested this method because it shaves some time >good luck:mushroom2:


--------------------
Carpe Diem


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InvisibleMad Season
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: sporeworker]
    #23731242 - 10/12/16 01:15 PM (7 years, 3 months ago)

Ms to agar is the same rate of colonization, and it will tell you if it's truly clean. Drop the wedge into LC or make LIs. Going MS > LC is like playing roulette really. I guess if you enjoy gambling, then keep on doing it. But here in shroomery we recommend the best way to get a clean grow. Recommending MS > LC is :justno: especially when there's contaminations involved here.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinesporeworker
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Registered: 07/21/16
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Last seen: 6 years, 1 month
Re: Started grains and agar. Neither are going too well, advice needed. [Re: Mad Season]
    #23731345 - 10/12/16 01:49 PM (7 years, 3 months ago)

fair enough.. always up to learn new things. guess iv just had really good luck this far so i havnt felt the need to change methods:grin:  have a couple dishes im playing with as well, il have to give agar>lc a shot & see the difference:wink:


--------------------
Carpe Diem


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OfflineMortySmith
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Registered: 03/28/16
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: Mad Season]
    #23731496 - 10/12/16 02:51 PM (7 years, 3 months ago)

Quote:

sporeworker said:
fair enough.. always up to learn new things. guess iv just had really good luck this far so i havnt felt the need to change methods:grin:  have a couple dishes im playing with as well, il have to give agar>lc a shot & see the difference :wink:



I think you have already seen the difference, all those times you had uncontaminated LC! The real difference is the odds, I think. Instead of a 1 in 10, 1 in 20, or whatever chance of getting a contaminated LC, its 0, because all your contams show up on agar and stay on agar :smile:

Quote:

Mad Season said:
That isn't cobweb (dactylium) that is some grey ass mold that wishes it could colonize as quickly as cobweb.

Oh and 6 days is nothing for germination on agar. In fact if it germinated quicker, often times it's some green ass mold that germinates quicker.



That is good to know! I was thinking it was only a few days lol, I must have been reading about transfers or something.


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InvisibleComebackKid
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23731566 - 10/12/16 03:14 PM (7 years, 3 months ago)

Quote:

mrknowitall95 said:
Quote:

sporeworker said:
fair enough.. always up to learn new things. guess iv just had really good luck this far so i havnt felt the need to change methods:grin:  have a couple dishes im playing with as well, il have to give agar>lc a shot & see the difference :wink:




I think you have already seen the difference, all those times you had uncontaminated LC! The real difference is the odds, I think. Instead of a 1 in 10, 1 in 20, or whatever chance of getting a contaminated LC, its 0, because all your contams show up on agar and stay on agar :smile:




The difference is in the yields. There's no way to make uncontaminated LC with MS unless you do agar first.
Odds are 100% bacrerial from spore syringe.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineMortySmith
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Registered: 03/28/16
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: ComebackKid]
    #23731607 - 10/12/16 03:26 PM (7 years, 3 months ago)

Quote:

ComebackKid said:
Quote:

mrknowitall95 said:
Quote:

sporeworker said:
fair enough.. always up to learn new things. guess iv just had really good luck this far so i havnt felt the need to change methods:grin:  have a couple dishes im playing with as well, il have to give agar>lc a shot & see the difference :wink:




I think you have already seen the difference, all those times you had uncontaminated LC! The real difference is the odds, I think. Instead of a 1 in 10, 1 in 20, or whatever chance of getting a contaminated LC, its 0, because all your contams show up on agar and stay on agar :smile:




The difference is in the yields. There's no way to make uncontaminated LC with MS unless you do agar first.
Odds are 100% bacrerial from spore syringe.




So you are saying even his "uncontaminated" LC was still bacterial? And he doesn't notice because it still produces fruit, just not as much as it would if it never had to be battling that invisible bacteria throughout colonization?


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Offlinesporeworker
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Registered: 07/21/16
Posts: 44
Last seen: 6 years, 1 month
Re: Started grains and agar. Neither are going too well, advice needed. [Re: ComebackKid]
    #23731641 - 10/12/16 03:38 PM (7 years, 3 months ago)

Quote:

The difference is in the yields. There's no way to make uncontaminated LC with MS unless you do agar first.
Odds are 100% bacrerial from spore syringe.



I can understand the benefits of agar>LC but how could odds of bacteria be 100%... How would it be possible to grow successfully if contaminated with bacteria? Also once mycelium colony is established in theory shouldn't that fight bacteria to a degree?  >>I could understand what ^Offlinemrknowitall95 sugested about energy being lost due to fighting but my samples are very clean & in addition my agar is inoculated with my LC & looks/grows great :confused:


--------------------
Carpe Diem


Edited by sporeworker (10/12/16 03:45 PM)


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Invisibleamidogen
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Registered: 05/07/16
Posts: 1,782
Re: Started grains and agar. Neither are going too well, advice needed. [Re: sporeworker]
    #23731763 - 10/12/16 04:28 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 11:26 AM)


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Offlinesporeworker
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: amidogen]
    #23731889 - 10/12/16 05:10 PM (7 years, 3 months ago)

That makes sense    learn something new all the time:thumbup:  have to use a piece of the agar i have going to try it out.. so instead of using inoculation port on lid just put in glovebox & add agar wedge if i remember correct?


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Started grains and agar. Neither are going too well, advice needed. [Re: sporeworker]
    #23732149 - 10/12/16 06:42 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 11:26 AM)


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Offlinesporeworker
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Registered: 07/21/16
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: amidogen]
    #23732282 - 10/12/16 07:44 PM (7 years, 3 months ago)

cool!  so in theory the difference would be MS LC vs isolate LC?  -weird question once agar wedge is added to LC mix & put on stir plate does wedge "desolve" into solution?


--------------------
Carpe Diem


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Started grains and agar. Neither are going too well, advice needed. [Re: sporeworker]
    #23732338 - 10/12/16 08:05 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 11:26 AM)


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InvisibleComebackKid
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: amidogen]
    #23732365 - 10/12/16 08:15 PM (7 years, 3 months ago)

Im not sure if it desolves. Only done LC once and I didnt notice if it disolved or not. Im going to assume no but would also like to know for shits


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Offlinesporeworker
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Registered: 07/21/16
Posts: 44
Last seen: 6 years, 1 month
Re: Started grains and agar. Neither are going too well, advice needed. [Re: amidogen]
    #23732738 - 10/12/16 10:39 PM (7 years, 3 months ago)

I wondered if we were on different pages for a sec lol  once i find my extra stir bars il give it a go (im curious if it dissolves myself:grin:), think ill just use a small wedge or 2 unless theres a reason you say to add the whole puck?  use agar for additional nutrients or just more myc. to go around?  so would this be considered a isolate LC or just a different method?


--------------------
Carpe Diem


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