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OfflineMortySmith
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Registered: 03/28/16
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Started grains and agar. Neither are going too well, advice needed.
    #23728829 - 10/11/16 04:56 PM (7 years, 3 months ago)

I think I messed up lol.

These jars were inoculated with PES Amazonian on either the 1st or the 2nd, I forgot to look at the time and it was late, but about 10 days ago. There are 7 total, but I only included pics of the 4 with the most growth.

15 of the easy agar dishes were prepared and sterilized on the 2nd and 3 of those were inoculated on the 4th, each with a different syringe. One was a B+ that I made myself with a spore print from my PF tek harvest a couple weeks ago, one was leftover PES Amazonian from the grain jars, and one was Treasure Coast.



This is just disappointing... I appear to be growing cobweb in my wbs jars and nothing in my agar dishes. I am really unsure what that says about my sterile procedure, as it was almost identical for both the grain jars and the agar. There was some water that had settled in the agar dishes after sterilization, I looked up what to do and people said dump the water when you inoculate. So I did that, but more condensation has settled in the from the sides since then. It is pooling on one side and I feel like the single drop of spore solution just ran across the plate and is in that little pool now, for some reason unable to establish growth... What do you think I did wrong?


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OfflineBuger
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23728857 - 10/11/16 05:08 PM (7 years, 3 months ago)

I thought cobweb only grows on top of substrate like the way it does on casings in some situations. I learn something everyday; I suggest that you wait on it untill you see traditional mycellia and then add a some 3% peroxide to kill cobweb and save the jar, but it is a little risky and unorthodox. Goodluck Comrade.

B


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InvisiblemushboyMDiscord
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: Buger]
    #23728867 - 10/11/16 05:12 PM (7 years, 3 months ago)

did you clean up the agar?

as in do some transfers to new plates? or just spore to agar to jar?

if so then you bring whatever funky shit is in the syringe to the plate and
to the jar and so on.


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InvisibleComebackKid
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: Buger]
    #23728875 - 10/11/16 05:15 PM (7 years, 3 months ago)

How did you prepair your grains? What did you inoculate with?

Your plates are probably fine. They always condensate. It takes a while for spores from a syringe to germinate. I've had spores take amost two weeks, just be patiet.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineMortySmith
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: mushboy]
    #23728912 - 10/11/16 05:27 PM (7 years, 3 months ago)

I prepared the wbs exactly as spitballjedi says to in his tek, and pressure cooked for 2hrs at 15psi. I used a spore syringe of PES Amazonian to inoculate all of the jars, and one of the plates.


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Invisiblewildernessjunkie
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23728945 - 10/11/16 05:37 PM (7 years, 3 months ago)

Quote:

I used a spore syringe of PES Amazonian to inoculate all of the jars,




Right there is where you went wrong. Spore syringes are rarely clean enough to do a direct to grain inoculation.

None of those jars look good to me. Work on your agar a bit to get those spores cleaned up. If its taking too much time or effort, it may be best to start with a new syringe.


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InvisibleComebackKid
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23728960 - 10/11/16 05:42 PM (7 years, 3 months ago)

Its probably contamination from your syringe.
How much solution did you use in each jar?

Also could be just me but the grains seem to be wet. Did you make sure they were dry to the touch before loading jars?


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Offlinewtfcrazymofo
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23729047 - 10/11/16 06:15 PM (7 years, 3 months ago)

Ms to grain don't work well.  Keep up the agar work and try to stick with 1,2 or 3 tops of different kinds.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineMortySmith
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: ComebackKid]
    #23729060 - 10/11/16 06:19 PM (7 years, 3 months ago)

No they were wet. Read SBJ's tek, its how he does it. He just soaks in hot water for 24hrs after rinsing, let the excess drain for 30 minutes, then loads into jars. It was a method linked from "The Noob Forum" and I liked the sound of not boiling/simmering, as I was afraid of the cracked corn making it all sticky, and it just sounded easier. I thought it seemed weird too, but after reading in the comments for hours and seeing him and other people claiming that the boiling was only necessary for rye berries, I said screw it, it wouldn't be recommended by Trusted Cultivators if it didn't get results.

And yeah, I am thinking the contam was in the syringe. I knew it was a risk since spores are dirty and I knew agar was the only sure way I could get clean spawn, but I tried anyways.
I will do 3 more plates, with just the Amazonian this time. I was stupid and forgot to label the first three, so who knows which is which! Maybe someday when they come to fruit, I can compare pictures and at least figure out which was the B+ hahaha. What do you guys suggest I do about the condensation pooling and such? It doesn't seem like it would be beneficial for my growth to start in a pool on the edge...


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Offlinewtfcrazymofo
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23729092 - 10/11/16 06:35 PM (7 years, 3 months ago)

Dump it before you knock it in the sab. 

I prep my wbs by poring boiled water on them pop a lid on, then wait an hour, drain until there is not more than a few drops, load and pc, after the cycle and the pressure drops on its own I take them out (still hot), shake them, and throw them in the sab. Thanks foo man.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineMortySmith
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: wtfcrazymofo]
    #23729116 - 10/11/16 06:42 PM (7 years, 3 months ago)

Yeah, other than the soak time and how hot the water was, that is what I did too. And I did dump the first three out, but more condensation pooled after inoculation. The plates are a bit uneven so it is all pooling to one side. Will the drop of spore solution end up on the side as well? They aren't that uneven, but enough to cause a pool on one side. I dunno haha.


Edited by MortySmith (10/11/16 06:43 PM)


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Offlinewtfcrazymofo
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23729133 - 10/11/16 06:46 PM (7 years, 3 months ago)

Flame you loop then drop solution on it to cool it and 1 drop to catch spores then touch it to the agar if you dont want a flood.  It dries out in a few days if it is only condensation.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineMortySmith
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: wtfcrazymofo]
    #23729162 - 10/11/16 06:55 PM (7 years, 3 months ago)

Quote:

wtfcrazymofo said:
Flame you loop then drop solution on it to cool it and 1 drop to catch spores then touch it to the agar if you dont want a flood.  It dries out in a few days if it is only condensation.




Interesting, I don't just let a drop land in the middle of the plate? I was told to use a flamed loop or scalpel for spores from a print, I did not know about using it with syringes though. I need to read more about making a loop :tongue:


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InvisibleComebackKid
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23729237 - 10/11/16 07:19 PM (7 years, 3 months ago)

Jeepers. Having my spawn that wet would urk me out. I only have experience with rye. Usually having wet spawn is asking for trouble.

You never mentioned how much solution you used in each jar... Obviously you shouldnt be nocking up jars with spores while youre working with agar but Im just curious.

I have a hunch that you used more than 1cc and that hunch needs to be put to rest :lol:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineMortySmith
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: ComebackKid]
    #23729311 - 10/11/16 07:51 PM (7 years, 3 months ago)

Quote:

ComebackKid said:
Jeepers. Having my spawn that wet would urk me out. I only have experience with rye. Usually having wet spawn is asking for trouble.

You never mentioned how much solution you used in each jar... Obviously you shouldnt be nocking up jars with spores while youre working with agar but Im just curious.

I have a hunch that you used more than 1cc and that hunch needs to be put to rest :lol:




It was just about 1cc. I did 7 jars and had barely over 3ccs left. Went much smoother than my first grow haha, I have refined my method of using the syringe pretty well after reading some threads about it.

When I first used a syringe for brf jars, I was like "Whoa there! That was more than 1cc in just the first hole!" because it just slid so easily. It was due to the way I was holding it in my hand haha, now I can use quite a bit more pressure and just have it slooooowllyy slide down as the drops form at the end of the needle until they drop off.

I think 1cc ended up being a few drops on average. Didn't really count, just watched where the plunger was at mostly haha.


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InvisibleComebackKid
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23729418 - 10/11/16 08:30 PM (7 years, 3 months ago)

Usually when doing syringe to grain you want to use as little solution as possible. A drop or two will suffice. 1cc is quite a bit more solution than you need to noc up a jar of grain.

My first grain jar I did I didn't do any research and used a whole syringe thinking it would colonize faster. My jar ended up looking a lot like yours do pretty quickly. What I didnt realize at the time was that all I had done was introduce my grains to a shittonne of bacteria before any of the billions of spores even had a chance to germinate.

With a spore syringe, less is always more.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineMortySmith
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: ComebackKid]
    #23729477 - 10/11/16 08:58 PM (7 years, 3 months ago)

Good to know. Why is it that brf cakes are usually ok with spore syringes then?


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Offlinesporeworker
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: MortySmith]
    #23729553 - 10/11/16 09:29 PM (7 years, 3 months ago)

take of a bit of a ms syringe & make a LC, save lots of trouble personally i cant find a downside to a good LC.. & you can isolate farther once you have a tested LC sample your happy with.    (I prefer the self injection port/micron filter premade lids)


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Carpe Diem


Edited by sporeworker (10/11/16 09:31 PM)


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OfflineMortySmith
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: sporeworker]
    #23729643 - 10/11/16 10:00 PM (7 years, 3 months ago)

I thought LC was almost impossible to tell if its contaminated? What would be the benefit of MS to LC over MS to agar, clean it up, then turn any one of the clean cultures into however much LC you need? Have you ever had any contaminated LCs?

I should have only done agar but I was eager to start grains so I took a risk. As long as you guys say the plates could possibly still grow I will keep watching them!


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InvisibleTuhdoww
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Re: Started grains and agar. Neither are going too well, advice needed. [Re: Buger]
    #23729700 - 10/11/16 10:14 PM (7 years, 3 months ago)

Quote:

Buger said:
I suggest that you wait on it untill you see traditional mycellia and then add a some 3% peroxide to kill cobweb and save the jar, but it is a little risky and unorthodox. Goodluck Comrade.

B




Yea I'd say that's a little risky...:uhoh:

I'm sure you've done this before with great results, huh?


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