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OfflineEdmunter
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Registered: 05/01/13
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Agar and isolates
    #23728007 - 10/11/16 11:12 AM (7 years, 4 months ago)

So what are your usual stages to get an isolate from spores.  I get confused about how many plates to take and end up with too many and the missus threatens to leave with the cat.

germinate
multi spore grow
clone
?????????


How do you continue on and isolate once you like one.

Do you take a wedge from a culture before using it for a grow?

Grow and take a clone from a shroom?

Use and culture and leave a little behind in the plate?


Once you have a culture u like u can then take load of wedges and keep them in the fridge till you need them?

How long can you keep that up for before you need to slant?

Edited by Edmunter (10/11/16 12:19 PM)

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InvisibleMoabfighter
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Registered: 12/13/15
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Re: Agar and isolates [Re: Edmunter]
    #23728014 - 10/11/16 11:14 AM (7 years, 4 months ago)

I'm curious too. I just started agar work.


--------------------
KSSS And PE WBS.
 

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OfflineEdmunter
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Re: Agar and isolates [Re: Moabfighter]
    #23728041 - 10/11/16 11:29 AM (7 years, 4 months ago)

This is interesting but not spelling out my problem

https://www.shroomery.org/forums/showflat.php/Number/21176491

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OfflineEdmunter
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Re: Agar and isolates [Re: Edmunter]
    #23730807 - 10/12/16 10:41 AM (7 years, 4 months ago)

:popcorn:

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