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InvisibleLlwr
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Registered: 08/11/16
Posts: 92
2nd tub
    #23725878 - 10/10/16 05:24 PM (7 years, 3 months ago)

Here's my second tub, finally got off my ass and got some tissue onto agar today.  I've been working with agar off colonized rye grains (bunch off plates ready to go) but I figure a clone is a little bit less of a crapshoot.



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InvisibleBoogieman47
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Registered: 03/05/16
Posts: 9,712
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Re: 2nd tub [Re: Llwr]
    #23725880 - 10/10/16 05:25 PM (7 years, 3 months ago)

Looks good man


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: 2nd tub [Re: Boogieman47]
    #23726593 - 10/10/16 08:46 PM (7 years, 3 months ago)

Syringe to grains?

Looks good man. Nice job.


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InvisibleLlwr
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Re: 2nd tub [Re: morty422]
    #23726669 - 10/10/16 09:10 PM (7 years, 3 months ago)

pf to grain,  finally gonna use the last 18 quarts of that spawn tomorrow and noc up some grain masters with agar after that.  no idea what's going to come from that as it wasn't a tissue sample, should be more consistent though.  I got 4 samples tonight so that's in the works.


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Invisiblemorty422
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Registered: 07/06/16
Posts: 988
Re: 2nd tub [Re: Llwr]
    #23726828 - 10/10/16 09:59 PM (7 years, 3 months ago)

Quote:

Llwr said:
pf to grain,  finally gonna use the last 18 quarts of that spawn tomorrow and noc up some grain masters with agar after that.  no idea what's going to come from that as it wasn't a tissue sample, should be more consistent though.  I got 4 samples tonight so that's in the works.




You say you got 4 tissue samples--so you grabbed 4 clones from that tub today?
Nice!

I've had much better luck with clones than anything else-which is a general consensus for many here IMO...

pf to grain. That's interesting... I haven't seen too much of this. Could you elaborate?


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InvisibleLlwr
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Registered: 08/11/16
Posts: 92
Re: 2nd tub [Re: morty422]
    #23728505 - 10/11/16 02:46 PM (7 years, 3 months ago)

Sure, I saved 4 colonized pf cakes, dumped the dry verm layer and sliced off about 3/8"  off the top of the cake with a sterile knife.  I then distributed the remaining cake between 7 rye grain masters with a flame sterilized fork, getting it red hot and wiping with 70% between each master.  Each quart colonized in about 5 days due to there being a shit ton of inoculation points.  One batch went bacterial so I was left with 21 grain masters. All the work was done in a sab.  I know people say pf cakes are dirty but I didn't really lose to much,  a few jars and one tub out of a bunch.  Probably due more to my lack of skill than the cakes themselves.


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InvisiblemushboyMDiscord
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Registered: 04/24/05
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Re: 2nd tub [Re: Llwr]
    #23728557 - 10/11/16 03:02 PM (7 years, 3 months ago)

Quote:

Llwr said:
Probably due more to my lack of skill than the cakes themselves.




:rofl: i know what you mean.


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