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Offlinenoppie
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Panaeolus copelandia goliath AGAR work
    #23724325 - 10/10/16 06:27 AM (7 years, 3 months ago)

Recently inoculated this PDA Agar with syringe. Accidentally push syringe more about 2cc. Here's what happened after 4 days.
Do you guys any opinion all of those are mycelium? Except that redish one.



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OfflinePinpapa
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Re: Panaeolus copelandia goliath AGAR work [Re: noppie]
    #23724364 - 10/10/16 07:34 AM (7 years, 3 months ago)

I would take a small slice off  the edge of new growth of several of the whitest growths, transfer those to clean agar plates and repeat till you can fill a plate with clean growth. But I am new to agar and so far I'm struggling to get a clean one myself.good luck


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"This isn't the right thing to do , so let's go"


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Offlinenoppie
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Re: Panaeolus copelandia goliath AGAR work [Re: Pinpapa]
    #23725403 - 10/10/16 02:26 PM (7 years, 3 months ago)

Why all these growths are brownish at the middle of them? Why they're not white?


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InvisiblebodhisattaMDiscordReddit
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Re: Panaeolus copelandia goliath AGAR work [Re: noppie]
    #23725521 - 10/10/16 03:16 PM (7 years, 3 months ago)

I would toss that plate its lost cause. If you're trying to transfer away from contamination you should have done it 3 days ago.

It really helps to plate the spores appropriately too

Quote:

bodhisatta said:
On spores and agar

Doing this dramatically helps your chances as well as softer agar

Trusted Cultivator said:
Quote:

Trusted Cultivator said:
Quote:

bodhisatta said:
Quote:

bodhisatta said:


take your spores to an inoculation loop. if you have a print then flame the loop dip in agar, pick spores off print and then swab the dish

if you have a syringe flame the loop and drop a few drops onto the loop to cool it down and then one more drop to load it with spores and then swab that








Quote:

bodhisatta said:
Try using an inoculation loop. Flame the syringe needle and the loop then squirt onto the loop to cool the needle and the loop. Squirt another drop to prime the loop. Then swab the dish.



Then you can see growth immediately, see contamination easier, grab healthy mycelium easier, and never worry about spore drops rolling all over a dish giving you germination everywhere at the same time.










also the agar link in my signature







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