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OfflineDinkinFlicka84
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Help with correcting spawning technique & Possible conatams? Pics included.
    #23724142 - 10/10/16 02:02 AM (7 years, 3 months ago)

In these first 2 pics it may not seem like an obvious contam, but I'm wary now that I know how insignificant contams can appear. This pic was taken 3 days after I noticed it. Wanted to see if it turned into trich, cobweb or other mold. BTW, I've smelled every jar before and after I broke them up and they all smelled like fresh mushroom. I've heard you can ALWAYS smell a contam before you spawn (T.C. Frank H.), but I've also heard you can't always smell it.

Set A
 


Is there a negative effect from spawning my monos this way?
I PC bulk 1hr, later mix near ratio [2qt WBS]:[3qt verm/3qt coir] inside tub for 3-5 minutes, use thin trash bag pulled up & over rim tub rim, & lock the lid on said bag. There'd be a good amount of moisture during this 5-7 day period before fruiting and seemed drops of water from the lid may have fallen into the substrate while colonizing because I've had to move the tubs around.

I've since changed the way I spawn my monos now by mixing 2:6 (1:3) WBS & coir/verm/gypsum in a stainless steel bowl(bleach/soap scrubbed), levelled out in tub, using 3.5mil clear liner, cutting 3 inches above the sub, duct tape all holes and using locking lids.



Would the sub having tons of tiny droplets covering the surface (below) or "wet spots" where fallen moisture "darkened" the surface (above) have an effect on contams?
Occasionally the bag would be loose and touch the edges of sub, which colonized fully. I was intrigued they were 100% in 5 days usually. Very fluffy on top, but there would be areas like in these pics that were not as fluffy or as colonized-seeming. These are the areas (above) I worry about, despite not seeing green after a few days. 

   

Re: PC'ing coir/verm/gypsum:
I know it's controversial, however, I can't imagine PC'ing or pasteurizing causing contamination either way, since coir simply provides structure/holds water and vermiculite has only trace minerals.


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OfflineMadSeasonStudent
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: DinkinFlicka84]
    #23724398 - 10/10/16 08:03 AM (7 years, 3 months ago)




2:6 ratio seems to be little high, no?

If you decide to pressure cook or pasteurize your sub
And you have dirty spawn; its not going to matter too much which way you go.


I sterilize usually but, some say pasteurization is better
because of the microbes arent totally killed off.

I followed Frank as well, he has some great growing ideas.

Longer it takes to colonize the chances of contam goes up
Thats my theory on it. Maybe 2:6 ratio is perfect. Ive always done 7 quarts of spawn
To 8 jars of sub thats 1:1 ration. 2:1 is good as well. Thats why i was wondering about 2:6 ratio not saying it wont work..i was just curious as to why.
Dude im so stoned forgive me if i seem off.


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InvisibleInocuole
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: MadSeasonStudent]
    #23724416 - 10/10/16 08:12 AM (7 years, 3 months ago)

You don't need your verm and coir to be 50/50..  That's a lot of verm, not enough coir.


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OfflineDinkinFlicka84
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: MadSeasonStudent]
    #23724453 - 10/10/16 08:30 AM (7 years, 3 months ago)

That's just it. All 30 jars were G2G from 1 single qt jar and they colonized in 5 days. It was the fastest I've ever seen them. The jars didn't smell off or anything. In fact, I posted pics of 10/30 jars fully colonized and had multiple members say they looked fine, including Cronic, a trusted cultivator on this site.

Now, most of my monos (1:3) are contaminated. I'll post pics here in a minute. I don't get it. I've been doing this for almost 5 years now and have had plenty of contams and then a good period with little to no contams. Now, I have no idea what could be wrong since the jars smelled fine and coir/verm are very contam resistant.

Also, when I spawned them (2:6) they also colonized 100% in 5 days. I even game them 6 just to be sure. But somehow, contams still go in there. I don't get it. Like you said, the longer the colonization time, the more change of contams.


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Edited by DinkinFlicka84 (10/10/16 08:39 AM)


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OfflineDinkinFlicka84
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: Inocuole]
    #23724459 - 10/10/16 08:33 AM (7 years, 3 months ago)

Quote:

Inocuole said:
You don't need your verm and coir to be 50/50..  That's a lot of verm, not enough coir.




Well, it's worked for me for a long long time. Plus, most of the coir/verm teks say 50/50. I see you have a shit-ton of posts, so I guess it's just personal preference. But I'm not saying you're wrong. I just know it's worked for me so far. In my sig, you can see the period where my agar, monos and GH were helping me produce nice, healthy fruits.


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OfflineDinkinFlicka84
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: DinkinFlicka84]
    #23724505 - 10/10/16 08:54 AM (7 years, 3 months ago)

I see now that TWO TC's said they looked fine and I'm just now going through every single tub and they're all contaminated for sure. I thought they looked off, but I think you have to really look closely to see that a lot of the individual grains of WBS are not fully covered in mycelium.

Which still boggles my mind because they smelled like mushroom when I opened the jars before spawning them to bulk. Is it even likely at all that I would get the odd contamination that doesn't show up after shaking the jars specifically to check if they're contaminated? Neither in how they look OR smell?

https://www.shroomery.org/forums/showflat.php/Number/23683176

Here's the post with pics of the jars.


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Edited by DinkinFlicka84 (10/10/16 08:55 AM)


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OfflineMadSeasonStudent
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: DinkinFlicka84]
    #23724536 - 10/10/16 09:10 AM (7 years, 3 months ago)



Well you have to go back to the start and pin point where it possibly become from

if it shows contam before the first flush, its a spawn issue.

Have you played around with the P.H levels to get a higher P.H?
I never had an issue at 8.5 PH


I soaked all my jars one time when i had an outbreak in hot soapy water and bleach for 24 then dishwashwr for a few times. That did the trick for me.

Are you soaking your seeds prior to pressure cooking?
Some of the wbs ive bought easily tammed out.

Could it be your pressure cooker?
Maybe a new seal or something would help
Ive been going longer pc time 3 and a half hours instead of the 90 minutes


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OfflineDinkinFlicka84
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: MadSeasonStudent]
    #23724570 - 10/10/16 09:23 AM (7 years, 3 months ago)

Quote:

MadSeasonStudent said:


Well you have to go back to the start and pin point where it possibly become from

if it shows contam before the first flush, its a spawn issue.

Have you played around with the P.H levels to get a higher P.H?
I never had an issue at 8.5 PH


I soaked all my jars one time when i had an outbreak in hot soapy water and bleach for 24 then dishwashwr for a few times. That did the trick for me.

Are you soaking your seeds prior to pressure cooking?
Some of the wbs ive bought easily tammed out.

Could it be your pressure cooker?
Maybe a new seal or something would help
Ive been going longer pc time 3 and a half hours instead of the 90 minutes




I'm in Denver, so I PC 120min. I don't soak my seeds simply because I usually don't and haven't had any problems. It's frustrating to do the same routine and get different results. I shook the jars to see if they would contam and they recovered and looked the same. But I've read that some contams require more oxygen to grow.

I've never heard much about pH from any teks I've read, plus I think I figured what the problem was. It was this last batch that I G2G'd from 1 jar. The time before, I did it from 1 qt and 1 pint jar. 8/10 tubs contammed, but 2 tubs are fine. I just realized that I should've kept each set of 12 jars separate of each other, in case one of the 2 jars I G2G'd from were contammed. So the fact that 2 tubs (4 jars) made it just fine, means that I mixed the rest of the jars up, and just happened to get those 2 sets of 2 jars from the non-contaminated G2G jar.

Also, you can't smell jars that you use for G2G. I need them as clean and free of any extra floaties going around my face, even with a mask and gloves.

I'm going to start over with agar. I've just had problems where I can't see or tell if mold is growing on my plates because they aren't always green or a different color than myc.


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OfflineMadSeasonStudent
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: DinkinFlicka84]
    #23724591 - 10/10/16 09:33 AM (7 years, 3 months ago)



I would still wash the jars just to make sure its not that.

I think you answered your own question somewhere in that.
Agar is a blessing in disguise for sure!

That PH level probably is all subjective
I know trich doesnt like higher P.H...that is why i mentioned it.
:crazy2::mushroom2:


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OfflineDinkinFlicka84
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Re: Help with correcting spawning technique & Possible conatams? Pics included. [Re: MadSeasonStudent]
    #23726237 - 10/10/16 07:09 PM (7 years, 3 months ago)

Quote:

MadSeasonStudent said:


I would still wash the jars just to make sure its not that.

I think you answered your own question somewhere in that.
Agar is a blessing in disguise for sure!

That PH level probably is all subjective
I know trich doesnt like higher P.H...that is why i mentioned it.
:crazy2::mushroom2:




Yeah, thanks for the suggestion. It's been 5 years and I'm still learning how to do this and every bit of info helps. Plus, anything I can do to stick it to trich, I'm ready for. Ruining my day by showing up in almost all my hard work. I'll look up the pH stuff and see where I might benefit from it.


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