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Offlineexperimentalscientist
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Starting up with agar question
    #23722146 - 10/09/16 01:01 PM (7 years, 3 months ago)

I have spores on sterile cotton swabs in test tubes, that have been sitting for about 4 months now from a previous grow. I am planning to simply swab the spores on to the agar with the cotton swabs. Are there any better ways of going about this? should I wet the cotton swab first or anything?

Thanks all!


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InvisiblebodhisattaMDiscordReddit
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Re: Starting up with agar question [Re: experimentalscientist]
    #23722188 - 10/09/16 01:10 PM (7 years, 3 months ago)

Use say 1-1.5% agar instead of 2%. Aka 10-15 grams per liter rather than the typical 20g/L agar

Otherwise swab away.

Don't scrape up the agar either. Swab across the surface.

Last link in my signature


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OfflineEdmunter
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Re: Starting up with agar question [Re: bodhisatta]
    #23722310 - 10/09/16 01:44 PM (7 years, 3 months ago)

When I swab spores onto a plate I take the lid off and swipe the spores in a S shape over the agar.

My trick is that I hold the plate upside down when I do this as mold spore fall and this way u get good results.

Unlock the lid remove the plate holding it upside down, swipe and bosh  replace the plate, lock.

This is al if you are using pasty easy's.  if not im not sure.


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InvisiblebodhisattaMDiscordReddit
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Re: Starting up with agar question [Re: Edmunter]
    #23722335 - 10/09/16 01:52 PM (7 years, 3 months ago)



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Offlinemrmazdarx9
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Re: Starting up with agar question [Re: bodhisatta]
    #23722432 - 10/09/16 02:24 PM (7 years, 3 months ago)

A search what engine you say what's that?:rofl:


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Invisiblemorty422
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Re: Starting up with agar question [Re: mrmazdarx9]
    #23722646 - 10/09/16 03:33 PM (7 years, 3 months ago)

Quote:

mrmazdarx9 said:
A search what engine you say what's that?:rofl:




We have a search engine?

:breakout:


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InvisiblebodhisattaMDiscordReddit
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Re: Starting up with agar question [Re: morty422]
    #23722650 - 10/09/16 03:36 PM (7 years, 3 months ago)

I think people know it's there I just think they skipped library day in 5th grade where they teach you about booleans and the advanced tab.


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OfflineHamHead
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Re: Starting up with agar question [Re: bodhisatta]
    #23722821 - 10/09/16 04:35 PM (7 years, 3 months ago)

Wait, what's a boolean again?


--------------------
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OfflinePortabellaFella 1
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Re: Starting up with agar question [Re: HamHead]
    #23723219 - 10/09/16 06:51 PM (7 years, 3 months ago)

It's related to the dooky decimal system


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InvisiblePastywhyteMDiscord
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Re: Starting up with agar question [Re: PortabellaFella 1]
    #23723399 - 10/09/16 07:39 PM (7 years, 3 months ago)

Quote:

PortabellaFella 1 said:
It's related to the dooky decimal system




0 . :poop:


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InvisiblebodhisattaMDiscordReddit
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Re: Starting up with agar question [Re: HamHead]
    #23723601 - 10/09/16 08:47 PM (7 years, 3 months ago)

Quote:

HamHead said:
Wait, what's a boolean again?



Good definition


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Offlinejngy2k
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Re: Starting up with agar question [Re: bodhisatta]
    #23723712 - 10/09/16 09:19 PM (7 years, 3 months ago)

Quote:

Pastywhyte said:
Quote:

PortabellaFella 1 said:
It's related to the dooky decimal system




0 . :poop:



Quote:

bodhisatta said:
Quote:

HamHead said:
Wait, what's a boolean again?



Good definition







Can you tell me if this is mold?


Edited by jngy2k (10/09/16 10:56 PM)


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InvisibleMad Season
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Re: Starting up with agar question [Re: jngy2k]
    #23723726 - 10/09/16 09:23 PM (7 years, 3 months ago)

There's some growths in there I wouldn't trust at all. Why is everything so wispy? What is your recipe?

Wait you're not even the OP... Make your own thread..


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Invisiblemorty422
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Re: Starting up with agar question [Re: Mad Season]
    #23723817 - 10/09/16 10:01 PM (7 years, 3 months ago)

Quote:

Mad Season said:


Wait you're not even the OP... Make your own thread..




Yarr, I spy me a thread pirate!

:spank:


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