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OfflineDerek
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Registered: 02/19/04
Posts: 158
Last seen: 12 years, 4 months
2 Yr Old Spore Prints, No growth for 14 days.
    #2372150 - 02/23/04 03:33 PM (12 years, 9 months ago)

Made 5 Puerto Rican Syringes, 5 Hawaiian Syringes. All from 2 year old spore prints, there were in an altoids tin, just found them a couple weeks ago.

Syringes were made in accordance with the most demanding sterility specs.

I innoculated 5 pf jars with Puerto Rican, no dice, 14 days ago, and not even a single sign of life.

5 Days ago, 5 pf jars and popcorn innoculated with Hawaiian, no dice either.

They?re in an incubator which stays in the 80-81 degree range.

Should I keep waiting for these kids or should I look around for new spore prints?


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InvisibleTHEBOSS
THE BOSS

Registered: 12/16/03
Posts: 1,252
Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: Derek]
    #2372160 - 02/23/04 03:34 PM (12 years, 9 months ago)

bump that temp up to 86 and just wait, mine where 3 years old and took awhile as well.
Keep us posted on how you do.


THE BOSS


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InvisibleMilkVein
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Registered: 08/27/03
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Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: Derek]
    #2372161 - 02/23/04 03:35 PM (12 years, 9 months ago)

if there is no cantams....just keep waiting....and try to get your temps up a bit


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Look at how the time goes past.
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OfflineDerek
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Registered: 02/19/04
Posts: 158
Last seen: 12 years, 4 months
Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: MilkVein]
    #2372196 - 02/23/04 03:42 PM (12 years, 9 months ago)

Will do.


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OfflineBen_Stein
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Registered: 08/16/03
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Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: Derek]
    #2372238 - 02/23/04 03:49 PM (12 years, 9 months ago)

Yeah, it's a waiting game.

Also, you might want to check viability of your spores in some liquid (Karo or dextrose). You should see germination a bit faster, and if they fail to germinate in there, the jig is up.


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Offlinevermonster
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Registered: 10/17/03
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Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: Derek]
    #2372710 - 02/23/04 05:50 PM (12 years, 9 months ago)

Did you let the spores re-hydrate for a day in the syringe
before you shot the jars?


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OfflineDerek
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Registered: 02/19/04
Posts: 158
Last seen: 12 years, 4 months
Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: vermonster]
    #2375084 - 02/24/04 09:55 AM (12 years, 9 months ago)

Alright, I switched from the utterly useless heated jar incubator to a tub-in-tub. Temps are now teasing around 86.

Syringes: I let the puerto ricans sit a day, and I let the hawaiians sit 2.

Viability: I know at least some of the spores should be viable, I had the very last remains of a golden teacher print in the same altoids container, i dumped it into a little bonsai tree with some left over grain and mycelium took started appearing within 3 or 4 days. Unless golden teacher spores are known to be more durable than other strains' spores.

Do you think I can avoid the karo techs? (Where I live, things like corn syrup are imported from another hemisphere, and are prohibitively priced.)

Thanks for the feedback.


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Invisibletripndicular
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Registered: 08/25/02
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Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: Derek]
    #2375115 - 02/24/04 10:10 AM (12 years, 9 months ago)

It gets kinda dicey when spores are that old , but you should gets some sort of germination , just give it some more time , watch for contams , and keep those temps around 86 . It is good you waited a day or two before inoccing . But just to be on the safe side , prep some more jars and use some fresher sproes . IMO agar is best chance to get some germination with prints that old , but even then it takes more time than normal . Once saw spores take a month to germ on agar , almost tossed them in trash when low and behold white fuzz was apperaing , and once it did it was like wild fire .
Good Luck
Trip n D


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So with that said here is our mission statement .

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OfflineDerek
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Registered: 02/19/04
Posts: 158
Last seen: 12 years, 4 months
Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: tripndicular]
    #2375155 - 02/24/04 10:25 AM (12 years, 9 months ago)

I have some new Ecuador and B+ prints coming in.

Hopefully I'll have some growth before they get here, since I have something like 18 jars in the incubator.


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OfflineDerek
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Registered: 02/19/04
Posts: 158
Last seen: 12 years, 4 months
Re: 2 Yr Old Spore Prints, No growth for 14 days. [Re: Derek]
    #2375668 - 02/25/04 11:04 AM (12 years, 9 months ago)

My jars look totally and completely sterile. Its quite a depressing sight.

I still check them twice a day. But everytime hope diminishes...


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