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OfflineThelostsinner
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Registered: 09/27/16
Posts: 10
Last seen: 6 years, 6 months
How BADLY did I MESS UP my INOCULATION? * 1
    #23718032 - 10/07/16 10:42 PM (7 years, 3 months ago)

You guys are going to LOVE this. Didn't see many threads like this so I figured I'd just post my terrible/sloppy attempt at inoculating jars of BRF substrate and see if you guys can guess on a scale of 1-10 the magnitude of this screw-up.

Here goes - woke up at 630 AM (morning after sterilizing my jars in PC) thinking I was going to squeeze a slow/delicate process into a half-hour time frame, as I need to be on the road to work in about 30 minutes. For starters, I'm still not sure if my PC sterilized my jars properly - it's a fancy digital one and is self-contained. Instead of manually adjusting my temps I had to use the pre-programmed "sear" setting for 90 minutes, hoping it would be enough.

The morning after I donned my sterilized gloves/mask after washing up, abd in the sterilized closet I once used for growing canna, I took my jars out, set them inside and prepared my spore syringe. I took the plastic tip off with my gloved hand and replaced it with a covered needle.

I removed the plastic cap and heated the tip of the needle until red-hot with a propane torch set on low. Here's where it gets messy - I realized I had punched the holes in my jar lids TOO DAMN SMALL, and the gauge of needle I was sent w/ my spore syringe was a hair too big.

I managed to do a proper inoculation on TWO of my FOUR jars, with flamed needle gently inserted facing glass and squirted in about 0.5ccs per side.

On my final two jars I did not manage to shove my needle through, and after trying to force it, snapped my needle in half. Thinking I had time to save the whole operation, I quickly removed the lids from my last two jars and shoved the syringe in past my dry verm layer and inoculated the rest of the spore solution.

At this point I stuck them in the top shelf of my closet. I'm regulating/monitoring the temps in my room and had a high of 86 deg. F today. I have since adjusted the temps on the space heater and gotten it down to a nice 76, however the RH in my room remains a very low 17%, with a 22% high.

I checked on them about two hours ago, about 16 hours after the initial inoculation, and don't see much of a change yet, however they've barely had time to set up shop.

So, are two of my jars DEFINITELY screwed? I hope I didn't buy a giant plastic tub and turn it into a monotub for no reason. :grin:

TL;DR used "PF Tek done right" guide and inoculated 2 jars w/ lids off because I snapped my needle off halfway through (due to too small holes in lids). lost 3 jars to contams so far, one still going strong. I'm a fucking noob.


Edited by Thelostsinner (10/14/16 11:56 PM)


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InvisibleMoabfighter
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23718040 - 10/07/16 10:47 PM (7 years, 3 months ago)

Holy shit.

I hate to say this, but this is probably the funniest, most terribly poor job I've ever heard at someone trying to inoculate PF jars.

Imagine OP doing this shit. Real life, he did this.


You're insane man, and none of your jars are going to make it.

To add some insight, atleast..

Your dry verm layer comes long before you inoculate. You add the dry verm to the top of your moist mix before you PC.

Two, shoving a spore syringe, without a needle, into your opened jars.... not a fucking chance that's ever gonna work.

Three, open air. No STill Air Box, no attempt to clean yourself/air/breath/anything.


Complete disaster. Throw those away, read, ALOT more, and try again.

Some learn faster than others, I feel it takes you abit longer to learn than some. That's okay. Just try extremely harder next time.


--------------------
KSSS And PE WBS.
 


Edited by Moabfighter (10/07/16 10:50 PM)


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23718046 - 10/07/16 10:51 PM (7 years, 3 months ago)

Wow man...

I wouldn't trust a lot of what happened during that inoculation.

I would start over and try to not have so many vectors of contamination.

Keep them jars around anyway-but I would re-up and start another BRF grow immediately.

There was a lot of bullshittery that happened.

Pushing the broken needle down through the verm... oh man.

:cheers: and re-start  --- but keep those jars around....you know...for science.


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: How BADLY did I MESS UP my INOCULATION? [Re: Moabfighter]
    #23718049 - 10/07/16 10:53 PM (7 years, 3 months ago)

Quote:

Moabfighter said:

To add some insight, atleast..

Your dry verm layer comes long before you inoculate. You add the dry verm to the top of your moist mix before you PC.




Shee-yit... Didn't even catch that shit.

Damn Moab... :lmafo:



OP - It's all good. We have all made some mistakes. Re-start and do it right this time. Plan a bit more accordingly and be a LOT more sterile...

haha.


:breakout:


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OfflineThelostsinner
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Registered: 09/27/16
Posts: 10
Last seen: 6 years, 6 months
Re: How BADLY did I MESS UP my INOCULATION? [Re: morty422]
    #23718079 - 10/07/16 11:04 PM (7 years, 3 months ago)

I knew this was going to be good for me, bahahaha! Also I feel I should edit the OP if it wasn't clear - I DID add my dry verm layer before my PCing, sorry if that was confusing for being so late in the tale.

Still gonna hang on to my first two jars that inocc'd well enough - plus aside from my two disaster jars I don't see how anything could have gotten in with a flamed needle and a small enclosed space w/ gloves and face masking. I suppose I figured the "cleaning up" portion was somewhat implied, so I left it out.


Edited by Thelostsinner (10/07/16 11:07 PM)


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23718092 - 10/07/16 11:08 PM (7 years, 3 months ago)

Quote:

Thelostsinner said:
I knew this was going to be good for me, bahahaha! Also I feel I should edit the OP if it wasn't clear - I DID add my dry ferm layer before my PCing, sorry if that was confusing for being so late in the tale.

Still gonna hang on to my first two jars that inocc'd well enough - plus aside from my two disaster jars I don't see how anything could have gotten in with a flamed needle and a small enclosed space w/ gloves and face masking. I suppose I figured the "cleaning up" portion was somewhat implied, so I left it out.




On a personal side of things, and speaking on your mental stature-Don't get attached to these jars. I would immediately view these as failures... Not trying to be mean or anything-I just don't want to see you let down.

Start new jars immediately. No joke. If your syringes are gone-order new ones.

If I had a print laying around, I would seriously send you one, but all my stuff is on agar.

For really though... count these as a loss and start new jars asap.

:cheers:


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OfflineThelostsinner
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Registered: 09/27/16
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Re: How BADLY did I MESS UP my INOCULATION? [Re: morty422]
    #23718122 - 10/07/16 11:18 PM (7 years, 3 months ago)

I agree 100% - ordered more syringes. :laugh:


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InvisibleLlwr
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23718162 - 10/07/16 11:41 PM (7 years, 3 months ago)

I broke a needle in a pf one time in the middle of doing 36 cakes, luckily I had a bunch of syringes handy.  After that I switched to single innoc point in the center like george does with his Kramer cakes.  Not that you should deviate from the pf tek, build a sab, and do it properly a few times.


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Invisiblemorty422
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Registered: 07/06/16
Posts: 988
Re: How BADLY did I MESS UP my INOCULATION? [Re: Llwr]
    #23718182 - 10/07/16 11:55 PM (7 years, 3 months ago)

Quote:

Llwr said:
I broke a needle in a pf one time in the middle of doing 36 cakes, luckily I had a bunch of syringes handy.  After that I switched to single innoc point in the center like george does with his Kramer cakes.  Not that you should deviate from the pf tek, build a sab, and do it properly a few times.




OP - I would say your best bet is to either take that syringe and begin to isolate cultures on agar-- or start new cakes with correct holes, etc..

I think you should start your progression with one of these two ideals. This is my opinion though, and not a fact.

:cheers:


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Offlinethebug76
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Llwr]
    #23718186 - 10/07/16 11:56 PM (7 years, 3 months ago)

I did that same shit once, except I had a SAB. Evidently, I wasn't driving the nails far enough through the lids to make the holes big enough. I use a drill and plastic lids now, much better.


--------------------
Bug 


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OfflineThelostsinner
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Re: How BADLY did I MESS UP my INOCULATION? [Re: thebug76]
    #23738350 - 10/14/16 07:52 PM (7 years, 3 months ago)

Small update - behold my ONE out of four jars that is so far sans contamination. The other three developed large patches of some kind of black mold type stuff and I tossed em. Well I guess fruiting one cake is enough to keep me entertained until more spores arrive.



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OfflineGreg
always learning
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23738373 - 10/14/16 07:59 PM (7 years, 3 months ago)

Why are you using a quart jar? That's going to stall before it's fully colonized.


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OfflineThelostsinner
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Registered: 09/27/16
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Greg]
    #23738662 - 10/14/16 10:12 PM (7 years, 3 months ago)

I was actually torn between the 1qt and 1/2qt jars at first but had seen guides that involved the use of both of them successfully and figured "eh, more sub more shrooms". I suppose I'll be waiting a bit longer for this one to fill up completely?

Either way, fun little project to keep an eye on while I wait for more spores.

Also in hindsight - I think I'll be buying a pack of the 1/2 qt jars here shortly - stacking full-sized jars inside of my PC was a B.I.T.C.H.


Edited by Thelostsinner (10/14/16 10:14 PM)


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OfflineGreg
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23738668 - 10/14/16 10:14 PM (7 years, 3 months ago)

I was under the impression that you were using the PF tek but then I read you PCd them, carry on lol.


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OfflineThelostsinner
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Greg]
    #23738672 - 10/14/16 10:15 PM (7 years, 3 months ago)

Oh snap now I'm confused - do you not PC your jars of sub with the PF tek?


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OfflineGreg
always learning
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23738683 - 10/14/16 10:20 PM (7 years, 3 months ago)

I was under the impression that you are supposed to steam PF jars?
Aren't PF jars supposed to have a verm layer too?

I don't really remember though, I only do bulk/grains.

I should honestly stop replying though, I'm really, really stoned :hehehe:


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OfflineYaMoonSun
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23738691 - 10/14/16 10:22 PM (7 years, 3 months ago)

I can't believe I read all that. I'd say 50/50 chance if you don't disturbed the dry layer.


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OfflineTerpfreak
❀Terpenes❀
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Re: How BADLY did I MESS UP my INOCULATION? [Re: YaMoonSun]
    #23738699 - 10/14/16 10:25 PM (7 years, 3 months ago)

Im so fucking confused. :barbershreds:


--------------------


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Terpfreak]
    #23738705 - 10/14/16 10:28 PM (7 years, 3 months ago)

Quote:

Terpfreak said:
Im so fucking confused. :barbershreds:



Tldr, "I inoculated two of my pf tek jars without a lid, am I fucked?"


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OfflineTerpfreak
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Re: How BADLY did I MESS UP my INOCULATION? [Re: YaMoonSun]
    #23738735 - 10/14/16 10:49 PM (7 years, 3 months ago)

You inoculated them with the lid off method or you literally took the lid off for a long period of time after inoculation? The fist one is okay. Second one. no. hell no.


--------------------


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OfflineThelostsinner
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Terpfreak]
    #23738854 - 10/14/16 11:43 PM (7 years, 3 months ago)

No no no no - I inoculated my first two jars "perfectly" didn't disturb my dry verm layer, inoculated w/ needle facing glass and squirted in about 0.5cc per side.

Once I got to my 3rd jar I tried to force the needle into the too-small hole and snapped the needle in half. On these last two jars I tried to quickly unscrew my lids and shove my half-needle past my dry verm and inoculate.

Needless to say those two jars PLUS one of my "properly" inocc'd jars were fucked and got thrown away.

The one in the pic above seems to be coming along nicely though!  :letyourspiritsoar:


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OfflineMysticMoteToter
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23738974 - 10/15/16 12:50 AM (7 years, 3 months ago)

Hell yeah man, haha just read the story and laughed with you (: reminds me of when i started lol, everything feels so hectic. Anyways, imo your already ahead of a lot of recent posters though b/c your honest w/ yourself and didn't deny that you compromised the jars. Tossing compromised stuff will get you alot further than hanging onto it, also i'm not sure how you'll be able to get the cakes out other than breaking the jars haha. :shrug: It's a learning experience haha

edit: i'm assuming you've seen these videos? in case not though heres a link to a solid PF tek
http://www.mushroomvideos.com/


Edited by MysticMoteToter (10/15/16 12:53 AM)


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Offlinethebug76
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23739025 - 10/15/16 01:26 AM (7 years, 3 months ago)

Quote:

Thelostsinner said:
Oh snap now I'm confused - do you not PC your jars of sub with the PF tek?





If you're using brf in your pf jars they can be steamed or pc'ed. If you use wbs, I believe they have to be pc'ed. I may be wrong on the wbs. Grinding it may make a difference, but I pc everything now just to be sure.


--------------------
Bug 


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OfflineSergeant Whiskers
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Thelostsinner]
    #23739338 - 10/15/16 07:23 AM (7 years, 3 months ago)

Quote:

Thelostsinner said:
Small update - behold my ONE out of four jars that is so far sans contamination. The other three developed large patches of some kind of black mold type stuff and I tossed em. Well I guess fruiting one cake is enough to keep me entertained until more spores arrive.






HAHAHAHA is that a quart BRF jar?
no way the myc is going to get a hold of all of this (maybe it could have if there was some gas exchange)
PF Jars are supposed to be 4 times smaller than this man
sorry dude but dont get your hopes up for this jar, start over and read some more beforehand

and make sure you throw the contaminated jars to hell, black mold is nasty


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OfflineNDStepp84
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Re: How BADLY did I MESS UP my INOCULATION? [Re: Sergeant Whiskers]
    #23739376 - 10/15/16 07:50 AM (7 years, 3 months ago)

Lol, even if that jar colonizes how are you going to get it out of the jar? It will stall though. Follow the tek with wide mouth half pint tapered jars.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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