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AK1000
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Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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I put some spore syringe on agar and at the first sign of white spots, I transferred it, is that ok?
#23712622 - 10/06/16 09:47 AM (7 years, 3 months ago) |
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Basically, I wanted to isolate and transfer at the EARLIEST sign of mycelium growth. It took 5 days for the spores (many of which can easily be seen as black spots) to start turning white. They were literally just white specks when I took them out and put them into their own plates. (I made 7 different plates from all the different specks.)
Normally I have waited until they were much more visible in size. Is it okay that I transferred a literal speck? It'll grow into mycelium I'm sure...
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
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Re: I put some spore syringe on agar and at the first sign of white spots, I transferred it, is that ok? [Re: AK1000]
#23712634 - 10/06/16 09:49 AM (7 years, 3 months ago) |
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If you transferred a "speck", then I'm not sure how confident you can be you transferred a culture, you should grow it out just enough to determine if it's untainted Myc, and no more than that.
But if you are sure it'll grow out, And you've already done it, you'll have to wait and see now.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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mrmazdarx9
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Registered: 05/15/16
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Re: I put some spore syringe on agar and at the first sign of white spots, I transferred it, is that ok? [Re: AK1000]
#23712637 - 10/06/16 09:50 AM (7 years, 3 months ago) |
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I let it grow out a bit more so I have a clearer view of the actual mycelium, if you've cut the speck out surely all you've done is move one spore to another plate transferring all shit with it.
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: I put some spore syringe on agar and at the first sign of white spots, I transferred it, is that ok? [Re: mrmazdarx9]
#23712642 - 10/06/16 09:52 AM (7 years, 3 months ago) |
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Quote:
mrmazdarx9 said: I let it grow out a bit more so I have a clearer view of the actual mycelium, if you've cut the speck out surely all you've done is move one spore to another plate transferring all shit with it.
Exactly.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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Re: I put some spore syringe on agar and at the first sign of white spots, I transferred it, is that ok? [Re: Mushierage]
#23712653 - 10/06/16 09:55 AM (7 years, 3 months ago) |
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Ah good point mrmazdarx... I did not think of that. I guess I will see what happens to these 7 plates! Next time I'll wait a bit longer as well.
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