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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23727862 - 10/11/16 10:10 AM (7 years, 3 months ago) |
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Perfect, I ordered it. When it arrives, I have everything. My mushroom cap is on Aluminium foil right now so I will have a sporeprint.
When the LME arrives, I'll make the agar and will start
I didn't fide an inoculation loop but bought a inoculation needle, I think it's the same.
Before I start in jars, I want so ask you: Do you use self-heeling silicon on your lids?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23728708 - 10/11/16 04:05 PM (7 years, 3 months ago) |
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Quote:
AlCapone2k said: Before I start in jars, I want so ask you: Do you use self-heeling silicon on your lids?
Ok, I think this question was stupid, since I have to lift the lid anyway to put in some agar slice
But I ask myself: Is it possible to PF-Tek with Agar? PF-Jars have a vermiculite layer on the top as a contamn barrier. When I throw a slice of mycelium agar on it, it won't colonize it, right? So I should start with Grain Cakes, right?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23728729 - 10/11/16 04:13 PM (7 years, 3 months ago) |
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To use agar on a cake skip the dry verm barrier and use a grain jar lid. Remove lid put agar wedge in. Obviously this must be done in a still air box or with a flow hood
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23728768 - 10/11/16 04:28 PM (7 years, 3 months ago) |
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Yes, of course. I realized my question was stupid when reading another tutorials
On thursday I will get my LME and then start my agar. Until then I hope my spore prints are ready. Some say dry them, you say don't dry them - it seems that I am forced to dry them because obviously the caps will have finished the sporing procedure beforce my LME will arrive.
So I will dry the spore prints a litte. After pouring the agar in my petri dishes and waiting for it to cool down, I will take my inoculation needle, sterilize it outside the SAB with a flame, let it cool down inside the SAB and then take my dried spore print and put some of the spores on my agar plate.
Of course I will take care not to open the lid of the petri dish to long.
Then I close the petri dish, wrap it in seran paper (parafilm is too expensive here) and wait until it colonizes the petri dish.
Am I right so far? Where shall I store the petri dishes for colonization? If I understood correctly, in a dark place at room temperature?
Regards bodhisatta
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23728940 - 10/11/16 05:36 PM (7 years, 3 months ago) |
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Doesnt have to be a dark place a shelf is fine. I cut through the saran roll to make short pipes like 3cm wide mini rolls.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23729011 - 10/11/16 06:02 PM (7 years, 3 months ago) |
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Fine, thank you.
Then I will go into Jars, but this will take some time yet. I hope I can ask you some questions then, well, not really questions, just your opinions e.g. which Jar Lids to construct and so on. But we'll see, first of all I have to finish my agar experiment :P
have a nice day/evening. Regards!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23729507 - 10/11/16 09:11 PM (7 years, 3 months ago) |
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Im around all the time so if you have a question i dont see send me a message
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23730589 - 10/12/16 09:10 AM (7 years, 3 months ago) |
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Thank you man, you are great! Some people here seem to be annoyed by noobs like me but you are always willing to help, even when the questions seem stupid.
Just a report: My first spore print is finished in the SAB, I just pulled off the cap and put some aluminium paper under the glass to let it dry now. The cap of the other sporeprint will be finished in two hours.
Since neither my LME nor my pressure cooker have arrived yet, I am forced to dry the spore prints, cause I can't start on agar today.
After the spore print is finished, do I have to dry it in the SAB or is it now save from contaminents? Is it possible to take it out of the SAB and put in a not sterlized box with some Calciumchlorid to dry? Or will it contamine then?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23730611 - 10/12/16 09:20 AM (7 years, 3 months ago) |
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Just take the cap off the foil. And then put the jar or cup back over the print till it dries. If the jar or cup is wet sterilize/sanitize a new one and put it over the top of the print. Then fold the foil up so a clean sterile foil surface covers the print. Fold it up like a note you give to girls when you're 12.
You're whole printing process should be done in the SAB.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23730770 - 10/12/16 10:28 AM (7 years, 3 months ago) |
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Thank you. Since the "free foil", which is not under the cup, is now about 24 hours in the SAB, is it better to take some new foil from the roll and put it on the dried print before folding it?
Or is it better to wipe some alcohol on the "free part of the foil", wait until it dries and then to cover it?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23731881 - 10/12/16 05:08 PM (7 years, 3 months ago) |
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I always make sure the foil i print on is big enough to fold up. And make sure enough of the foil stays in the clean/sterile environment that i have enough excess foil to fold and cover the print
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23733145 - 10/13/16 04:46 AM (7 years, 3 months ago) |
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Clean/Sterile environment is the SAB or the part covered by the cup/jar put on the foil?
Because unfortunately the jar is just as big as the print so the area to fold on it was outside the jar.
So I better take new foil from the roll and put it on the print?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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