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InvisiblebodhisattaMDiscordReddit
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722254 - 10/09/16 01:28 PM (7 years, 3 months ago)

I would be surprised if REWE or someone couldn't get it for you.

Quote:

Prints are pretty clean because they're 99.9999999% spores even if a few contaminants get on there they're flooded out by numbers if used appropriately(like don't inoculate a LC with spores)




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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
    #23722268 - 10/09/16 01:34 PM (7 years, 3 months ago)

haha, so you seem to know Germany well :wink:
I was in REWE yesterday, they didn't even know what I'm talking about. I asked in an "organic store", there they call it "vegane gelatine" :laugh:
But they don't have it here. Tomorrow my last chance are some Asia markets, maybe I can get some.

Funny, I wasn't even able to get petri dishes since the pharmacies around here don't sell them. Very strange. But theres one left I will visit tomorrow.


What is meant by the last sentence:

"if used appropriately(like don't inoculate a LC with spores)"


Does that mean it is not appropiate to inoculate a LC with spores? Why not?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisiblebodhisattaMDiscordReddit
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722295 - 10/09/16 01:41 PM (7 years, 3 months ago)

LC is a 3D media, any single contaminant that gets in grows through the whole thing and ruins it. spore prints are never 100% clean due to the way they're obtained and made. though a spore print may be 99.9999% spores and 00.0001% contaminants it will be just fine if you start it on agar as the contaminant likely wont even survive being overcome with mycelium or it does grow and you can just take healthy mycelium to a new dish and leave the contaminant behind,

same goes for PF cake, you can squirt a spore syringe in there, and say a contaminant or two gets in, it probably will not be able to take over at all in most cases.
but a single contaminant will wreck a whole LC and you will not necessarily be able to tell at all until it ruins one of your grows.

you would probably find petri dishes where hospitals breweries(quality control and assurance) and farms(dairy and milk quality assurance and control)

there's probably some scientific supplier you can order from.

I would be surprised if apotheke had petri dishes


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InvisiblebodhisattaMDiscordReddit
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
    #23722325 - 10/09/16 01:48 PM (7 years, 3 months ago)

Wir Weichsel Weiber wollen wissen wo warmes Wasser wär womit wir waschen wollen.

Wir Wiener Waschweiber würden weiße Wäsche waschen, wenn wir wüßten wo warmes Wasser wär.

:shrug:

god damnit germnans

dont go over the geschwindigkeitsbegrenzung when driving around either


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722343 - 10/09/16 01:55 PM (7 years, 3 months ago)

haha your German is amazing :laugh:

Are you from the US?


Ok mate, I'll try to get petri dishes and agar agar tomorrow and then will inoculate agar on a petri dish. If I can't get it here, I must buy it online, also no problem.

But I have to be fast because I think tomorrow evening my mushrooms will open the cap. If I can't get agar agar to make my own agar dishes, maybe I'll buy one which is already made, I found a shop selling those with guarantee to be delivered one day after order. It's agar plate sterilized and everything's ready for inoculation. Maybe this can help me until I reveice agar agar to get my own :smile:

In school we worked with agar recipe made by Kimmig oder Kimming, I still have an explanation sheet how to make it here, can I use this for mycelium? :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/09/16 01:57 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722360 - 10/09/16 02:00 PM (7 years, 3 months ago)

never heard of it, get brewing malt, soak it in 150F water for 20-30m strain it out. water it down till its pretty light or if you have a hydrometer get it to like 1 to 2 plato.

or buy dried malt extract powder from a brewing store. use 2% by weight powder and 2% by weight agar-agar and well we know plain water is 1ml=1g for these purposes so like 20g of each to 1L water.

I've been to germany for a few weeks couchsurfing once.


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722370 - 10/09/16 02:03 PM (7 years, 3 months ago)

i dont know shopping details of germania but
here in Merica hitting up a asian supermarket
in the dessert aisle of all places you will
find years worth of agar agar for only a few
dollars. or euros whatever


Edited by mushboy (10/09/16 02:09 PM)


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722377 - 10/09/16 02:06 PM (7 years, 3 months ago)

Kimmig-Agar:

Peptone, Natriumchlorid, Glucose, Agar-Agar 15,0

Its specially made for mushrooms.


But I will use your recipe cause it's easy to get malt extract :smile:, easier than Peptone :laugh: :laugh:



For the fact that you only been here for some weeks, your german is quite good :laugh: Nice!


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisiblebodhisattaMDiscordReddit
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722387 - 10/09/16 02:12 PM (7 years, 3 months ago)

YPD is good for mushrooms too, malt extract agar( MEA) is mostly what I use

YPD would be better than the kimmig,
Yeast(hefe) peptone dextrose. the yeast is dead yeast for nutrition, sometimes called nutritional yeast.

the media you listed includes Natriumchlorid or salt which is something no mushrooms want in their media.


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
    #23722440 - 10/09/16 02:26 PM (7 years, 3 months ago)

Ok, then I forget Kimmig. Just read it on the instruction sheet as to be good for mushrooms :smile:

I'll make it walt extract.


I still have a question to spore syringes:

When I have a spore print, do I have to dry it before getting it into the spore syringe? :smile: Or can I put fresh prints into spore syringe?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722478 - 10/09/16 02:39 PM (7 years, 3 months ago)

You can make a syringe right away


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722493 - 10/09/16 02:43 PM (7 years, 3 months ago)



--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23722510 - 10/09/16 02:50 PM (7 years, 3 months ago)

Should be just fine


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
    #23722621 - 10/09/16 03:24 PM (7 years, 3 months ago)

its either white mold or its just stressed, mold pretty common in pf tek, but i wouldn't count on it, just leave it be and see what happens


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Edited by acidninja (10/09/16 03:24 PM)


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: acidninja]
    #23723056 - 10/09/16 06:06 PM (7 years, 3 months ago)

Quote:

TheAcidJunky said:
its either white mold or its just stressed, mold pretty common in pf tek, but i wouldn't count on it, just leave it be and see what happens





Until now there are 4 mushrooms, two very big and zweo quite small. So I think this Flush is not that powerful like the first one, I will try a third flush but I'm not expecting much.

With a growkit, is it possible after letting it rest for 24 hours with water to initate the third flush to remove the case and put the whole cake upside down in a fruit chamber? Could this lead to a better third flush?


--------------------
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My small Agar to Oyster Open Air Grow Trek


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
    #23723086 - 10/09/16 06:15 PM (7 years, 3 months ago)

Quote:

bodhisatta said:
Should be just fine





I have two questions to your pour agar tek:


1. You say "sterilize your agar for 15-20m at 15PSI. [..] once your PC is back at atmospheric pressure. open it up take your jars out. (tighten the lids down)"

Does that mean that I dont close the bottle of the agar solution when putting it into PC? Just put the lid on but do not tighten it?


and

2. "pour your plates. use your wrist, don't let your hand ever go above anything sterile."

You really mean to open the petri dishes and to pour the agar from the bottle into them and then close them again without my hands, just with my wrists?


and

3. After pouring agar on and putting the lid back on the petri dish, can I just wrap it in saran wrap and it will stay sterile? Or do you prefer parafilm? :smile:



Sorry for the lot of questions but sometimes I don't unterstand parts of the teks you describe so better I ask than making mistakes which frustrates me for sure :P


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/09/16 06:20 PM)


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
    #23724857 - 10/10/16 11:10 AM (7 years, 3 months ago)

Sorry, wrong post!


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/10/16 11:31 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23727158 - 10/10/16 11:57 PM (7 years, 3 months ago)

Usually when you're sterilizing liquids in bottles in an autoclave(lovely English)

The bottles have caps, but most bottle caps do not have special gas exchange lids. Like PF cakes, or grain spawn jars. Those have holes in the lids and some sort of filter. If you autoclave something with no filter the lids are loose during the cycle then tighten it down afterwards. This is typical laboratory procedure. There's many ways to slice a cake though. If you don't have a way to exhaust your jars as they heat up the gasses dissolved in solution become less soluble and there is already air in the headspace so the pressure in the jar reaches higher pressure than even the atmosphere in the pressure cooker and you can pop jars. Big mess if it happens.

You understand our English really well or only kind of? I can try messaging you in some German too if English isn't so good. But your English is way way better than my German.


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
    #23727745 - 10/11/16 09:13 AM (7 years, 3 months ago)

Thank you very much, now I understand.

I have everything now but one thing is missing:Light Malt Extract. I was in 10 shops and can't find it.

I have to order it online.

Is this the right stuff:

http://m.ebay.de/itm/Muntons-Malzextrakt-Hell-aus-Gerstenmalz-500-g-Bier-selber-brauen-/261778251589?nav=SEARCH

It's Muntons Spraymalt light, usually used for brewing.


--------------------
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My small Agar to Oyster Open Air Grow Trek


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23727785 - 10/11/16 09:32 AM (7 years, 3 months ago)

That is the stuff.

You can use amber malt extract too or even rye malt extract

Light is just the most clear. But most people end up using food coloring to make if darker anyway. There's less simple sugars in amber extract the mycelium does just fine on it.


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