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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: Mushierage]
#23713011 - 10/06/16 12:13 PM (7 years, 3 months ago) |
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Thank you very much for your answers.
I have heared that one of the biggest problems of agar cultivation is the production of the agar plates.
Is it possible to just buy ready-to-use agar plates like these ones and use them:
https://www.medplus24.de/nutriplate-fertignaehrboeden-pilz-agar-nkimmig.html?gclid=CKrJ78rjxs8CFRPgGwodygEPAg
These are Agar Plates (Kimmig Style) specially made for mushroom cultivation. 20 cost only 15 Euros, that is very cheap and saves a lot of time. Do I have to sterilize them in a steam pressure cooker before using?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23713026 - 10/06/16 12:18 PM (7 years, 3 months ago) |
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Don't buy pre made plates. Give up on agar if you're too afraid to make your own. It's the damn near easiest thing you'll do. Pour agar into dish. Boom done easy.
You cannot sterilize petri dishes they melt. They come sterilized by gamma irradiation
Last link in signature has all the agar teks that dont suck in one place
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23713474 - 10/06/16 03:02 PM (7 years, 3 months ago) |
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Hey,
I am not afraid, I just read in a discussion on a German forum, that it's not smart to start with agar, because it's more for advanced users. But I will give it a try!
I will read the agar teks in your sig!!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23721522 - 10/09/16 09:14 AM (7 years, 3 months ago) |
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Hey guys,
my second flush is now quite ok, there are just 4 Mushroom that came in the second flush, two are very big and two are quite small. I think I messed sth up while harvesting the first flush so my cake is stressed or so - therefor this flush is very "poor". But no problem, I just want some mushrooms to make a spore print anyway
I read a lot of teks and I think this one is the best: https://www.shroomery.org/8404/Spore-printing-pictorial
But in this tek it says:
"Position the glass carefully: make sure it does not completely seal the spore-dropping zone, so humidity can escape from a gap at the edge of the rim. either this, or make sure the foil is entirely enclosed within the glass: in at least one spot, the glass needs to rest on the teatowel, rather than creating an airtight seal (see on the right, in the pic above). The cap needs to be able to lose humidity slowly. As the cap dries, it sheds spores easier, like in nature."
This wonders me a bit since most of the other teks recommend to put the spore-throwing cap in a sealed environment for the print. And afterwards to lift the glass/container a bit to let the print dry.
What do you think, especially @Mad Season and @bodhisatta
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23721570 - 10/09/16 09:39 AM (7 years, 3 months ago) |
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Don't read any methods or teks off the main site it's all old shit. Look up let's grow mushrooms spore printing
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23721629 - 10/09/16 10:07 AM (7 years, 3 months ago) |
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Quote:
bodhisatta said: Don't read any methods or teks off the main site it's all old shit. Look up let's grow mushrooms spore printing
Quote:
bodhisatta said: Don't read any methods or teks off the main site it's all old shit. Look up let's grow mushrooms spore printing
You mean that site: http://www.mushroomvideos.com/Sporeprinting-Syringes?
I watched the video there and he closes the holes of his SAB after putting on the caps so that no humidity can get out.
So I planned to do so:
1. Put some aluminium foil in a jar and make an aluminium lid on the jar and put it in the oven on 200° for an hour or a bit more. Then I take out the jar, let it cool down a bit and bring it into my SAB.
2. I take some kitchen paper or something and put it in the SAB, and spray a bit of desinfection spray into the box and close the box lid. Then I take out one foil of the jar and put it on the kitchen paper, get my mushroom from the growkit, cut of the stem with a new sterile scalpel and put it with a pin sticked into it on the alu foil.
3. I cover the whole stuff with a small glass I sanitized with 70% ISP and take care to seal it. Wait 24 hours at least until the spore print is made.
4. Take of the glass, take the cap away and let it dry.
Now .. shall I dry it with a glass on the foil or leave the glass, just the uncovered foil in the SAB (with armholes closed)?
I am a bit confused now.
But this tutorial to my SAB I can follow, right?
https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23721652 - 10/09/16 10:15 AM (7 years, 3 months ago) |
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No I mean this stuff https://www.shroomery.org/8404/Spore-printing-pictorial Things that got put on the main site a decade ago. Good info is on the forums.
Mushroom videos.com is one of the places we say please go there look at that stuff instead.
And that SAB tek is one of the standards
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23721675 - 10/09/16 10:25 AM (7 years, 3 months ago) |
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So you mean because the printing pictorial I linked is from 2003 I should watch for the good stuff in the forum. Ok, thanks
Do you have a pictured tutorial which is new and helpful for a spore print made in a SAB?
Mushroomvideos.com is not very detailed unfortunately.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23721689 - 10/09/16 10:29 AM (7 years, 3 months ago) |
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Mushroom videos.com spore printing is the standard. That one may be old but at least it's right.
Its not super detailed because all you're doing is putting a cap on a piece of foil
Just do it in the SAB like he does Infront of the flow hood
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23721999 - 10/09/16 12:20 PM (7 years, 3 months ago) |
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Ok, since I only have these few mushrooms to make a print, I am a bit afraid of ruin it, sorry for asking so much questions.
If I can't get agar agar until tomorrow I am forced to make a spore syringe and I am afraid of making a contaminated spore print.
Have I understood you correctly, that wiping out alumunium foil with ISP is useless, better to sterlize it in the oven with 200°C for one hour and then directly sporeprinting on it?
You don't put any glass oder something on the foil+cap while the spores are falling down?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722013 - 10/09/16 12:24 PM (7 years, 3 months ago) |
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Foil is sterile off the roll. Wiping something sterile with alcohol is like smearing shit on yourself after taking a shower as one famous mycologist put it.
Take a few inches off the roll save itnfor something else. Then the next piece you take you print on. Did you watch the video?
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722110 - 10/09/16 12:49 PM (7 years, 3 months ago) |
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Yes, I did. The part where it's about "indoor prints". I think I will do it like in the video, because its easier than making the print under a cup and have to take care when it dries.
Ok tomorrow I will get new aluminium foil, because my foil for cooking and so on is quite old and sure not sterile anymore New foil should be totally sterile then
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722114 - 10/09/16 12:49 PM (7 years, 3 months ago) |
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Contamination can't wiggle its way through the coil. Remove a foot or so its fine. The box its put in and the shelf its on at the store makes getting a new box a moot point anyway
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722131 - 10/09/16 12:55 PM (7 years, 3 months ago) |
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Good argument. Now I just hope that aluminium foil is not only sterile in the US and in Germany it's not
But I think you are right, if I remove a big part of the aluminium foil and take some foil from the interior of the roll it should be ok.
Do you cover your caps on foil with small glasses or something or do you exactly do it like in the video?
Regards.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722150 - 10/09/16 01:02 PM (7 years, 3 months ago) |
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I put foil down in the SAB then the caps then a Tupperware wiped down with isopropyl or pour boiling water into it as the lid.
Foil is sterile due to its manufacturing process. Surely your foil is made in the same few countries anyone's is.

You can do it outside the SAB too but the foil that's outside the boundaries of the "lid" device shouldn't be folded up on the sterile material
Sometimes I take the caps and move them to a new foil after a good print is obtained on the first foil. The second one I assume to be a little more clean as any contamination probably got on the first print. I keep the first ones to myself and trade the ones done on the second foil in the SAB to be more clean about it all. The first ones never give me a problem anyway. Prints are pretty clean because they're 99.9999999% spores even if a few contaminants get on there they're flooded out by numbers if used appropriately(like don't inoculate a LC with spores)
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: bodhisatta]
#23722163 - 10/09/16 01:04 PM (7 years, 3 months ago) |
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Ok fine, I'll make it this way.
And after 24 hours, you remove the cap. Ok. But do you put the Tupperware-Lid back on the print or do you lift it on one side for air circulation or do you just put the tupperware lid away and let them dry in the sab unconvered?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722181 - 10/09/16 01:07 PM (7 years, 3 months ago) |
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You'll be fine
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722196 - 10/09/16 01:11 PM (7 years, 3 months ago) |
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Yeah, thanks, I think so. I once worked during an internship in a laboratory so I am not totally nooby in sterile work, but I forgot a lot.
I would not get on your nerves with so many questions, but as I said, I only have 3 mushrooms for spore printing, if I fail, I have to get some sporeprints or a syringe from somewhere else, which is quit difficult in germany So I don't want to mess up.
I will let the prints dry in the SAB with the tupperware box upon them, but lift it a bit with some piece of sterile paper for drying (air circulation)
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722220 - 10/09/16 01:18 PM (7 years, 3 months ago) |
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Even a shit done print will be fine on agar.
Time to go to on a shifffhart Or to the Reeperbahn on an ICE train.
 Forget about mushrooms for a few hours
I thought you were using these print right away. Why dry them. Why do fancy extra stuff to dry them
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
#23722246 - 10/09/16 01:26 PM (7 years, 3 months ago) |
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haha, fine that you know the Reeperbahn, I'd go there, but it's far away from me right now But next time I'm there, I will remember your tipps
And yes, If I get agar agar tomorrow (will visit some asia stores) I won't dry it
Or maybe I make a liquid culture if I can't get agar agar, then I can direcy put some undried spores in the jar with my LC. We'll see
Regards!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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