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OfflineAlCapone2k
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B+ Growkit, first flush great, second flush with contamination?
    #23712429 - 10/06/16 08:35 AM (7 years, 3 months ago)

Hey guys,

is this a contamination in my box? It's the second flush, the first one was great and I harvested about 120grams of mushrooms.

After rehydrating the case with water and putting it back to the filter bag the surface of the box show this white fuzz. There is no strange smelling on the box, it smells a bit like old water in the filter bag but the case itself has no strange smell.









--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/06/16 08:42 AM)


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Offlinetump
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712441 - 10/06/16 08:41 AM (7 years, 3 months ago)

Doesn't look good


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: tump]
    #23712447 - 10/06/16 08:43 AM (7 years, 3 months ago)

Really? In other forums it has been said, that it's absolutely normal, that the mycell makes this white stuff after rehydrating.

This white stuff is exactly there, where there has been some rests of the mushrooms I harvested. Could it be, that the mycelium "eats" the rest of these mushrooms?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/06/16 08:45 AM)


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InvisibleMad Season
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: tump] * 1
    #23712451 - 10/06/16 08:45 AM (7 years, 3 months ago)

Quote:

tump said:
Doesn't look good



Yeah it looks stressed eh?

It's not exactly contaminated yet, but it isn't healthy. My guess would be bacteria. Bacteria always hits and weakens the network before molds can germinate.


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: Mad Season]
    #23712456 - 10/06/16 08:47 AM (7 years, 3 months ago)

Do you mean by stressed/weakened spots this white fuzz?
Or the places on the layer where there isn't any white mycelium?


There is no strange smell, the whole case smells a bit like mushrooms, shouldn't bacteria produce a strange smell?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712469 - 10/06/16 08:51 AM (7 years, 3 months ago)

Pseudomonas aeruginosa smells like flowers/grapes Streptococcus milleri smells of browned butter.

There's many bacteria with good smells, and some with no smells :shrug:

But what makes it look bacterial is the myceliums reaction to the environment. It's puffy and thick like cottage cheese. This is a reaction to bacteria.


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712474 - 10/06/16 08:54 AM (7 years, 3 months ago)

Thank you very much for your answer. Do you think the mycelium will be able to defeat the bacteria?

And if not: How will it develop in the future? What is the next step, that let's me know, that it's contaminated?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712489 - 10/06/16 08:59 AM (7 years, 3 months ago)

Never have I seen mycelium defeat bacteria. This is just the order of things. Bacteria opens cubensis up for trich to come in, and fully decompose it back to the soil.

It's just a matter of time til it molds. That being said, you can probably see another flush or 2 before it molds. But I'm just warning you to keep an eye on it for molds to appear.


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712499 - 10/06/16 09:02 AM (7 years, 3 months ago)

Ok, thank you very much. I will keep an eye on it, look for strange smells and so on. If the color changes, I will post a picutre here.

Is it possible, if it's not molding, that I can make a spore print of one of the comming mushrooms? Or will it be hopeless, because of the bacteria?


Thank you.


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Since I am from Germany, my english is not perfect but I try my best :smile:




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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712501 - 10/06/16 09:03 AM (7 years, 3 months ago)

Definitely possible.


--------------------
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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712511 - 10/06/16 09:06 AM (7 years, 3 months ago)

Ok, since you are an advanced member here I am using the chance to ask you a question, that wonders me after reading a lot on spore prints.

While making the print, one works very sterile. And then I take a mushroom which is definetely not sterile, remove the cap and put it on the sterile aluminium foil. At least then it's not sterile anymore?

Or is the fruit body of the mushroom always sterile when it's grown?


Would you recommend to make a spore syringe out of the spore print after drying?


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/06/16 09:08 AM)


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712516 - 10/06/16 09:09 AM (7 years, 3 months ago)

No mushroom cap grown in open air will be sterile. Thus no print will ever be sterile. Including vendor syringes/prints.

Do try to be as sterile as you can be when printing. Do it in a still air box. If you're worried about the cleanliness of it and contaminations for the future, get into agar. With agar you can cut out clean mycelium from contaminations. With agar all you need is a cotton swab or loop. No syringes needed.



https://www.shroomery.org/forums/showflat.php/Number/18430998/fpart/1/vc/1


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712529 - 10/06/16 09:14 AM (7 years, 3 months ago)

Thank you very much for the tip on agar. Yesterday I was in a pharmacy here and asked for agar plates and so on, so I am willing to start on Agar.

I red a lot about it but some are writing, that making spore syringes is much more easier than working with agars as a total noob like I am.

But I will try. The next days I will build a glove box. I have read some tutorial, do you have a tip for a good tutorial?


And two questions:

1. After having agar plates, I just put the spores on it from a spore print? Or do you recommend cloning?

2. Whih desinfection spray do you recommend? I have one from a drug store here but it's written on, that it kills mushrooms, so I think its not good for growing mushrooms. Is 70% Isopropanol enough?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712541 - 10/06/16 09:21 AM (7 years, 3 months ago)

I have no disinfecting spray. I use soap and water to spray down the sides of my SAB. Haven't used alcohol for a long ass time too.

Agar is so easy. Easier than the pf tek. It most certainly should be for noobs. Hell if intro level bio students can work with petri dishes, so can you :smile:.

Yes put spores on the surface of the agar. You can use a cotton swab if you want. I'd only put 1st flush clones on agar. You're too late for cloning.

https://www.shroomery.org/forums/showflat.php/Number/22556356


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: Mad Season]
    #23712549 - 10/06/16 09:25 AM (7 years, 3 months ago)



Mines doing same thing. Looks like green mold coming in middle of left tray. Watch for mold. It's coming. Glad I got one flush though.


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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712553 - 10/06/16 09:26 AM (7 years, 3 months ago)

Thank you very much for your answers, you helped me a lot.

So I can forget cloning now, no problem, next time I clone directly.

Maybe I will still earn a flush from this growbox before it's totally contaminated.

Then I will make a sporeprint and then put it on agar.

Do I have to wait until the spores are dry to put them on agar or can I directly brush them from the spore print after it has been set on the foil?

For syringes one has to wait until the spores dehydrate, If I understand it correctly, that is not possible on agair so I have to take fresh spores, right?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




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InvisibleMad Season
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712565 - 10/06/16 09:29 AM (7 years, 3 months ago)

Always let prints dry out before doing anything. It kills a lot of the bacteria present.

You print in a SAB. Print for 24 hours, then carefully remove the caps, and leave the prints open in your SAB for another 24 hours to dry out


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineAlCapone2k
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712590 - 10/06/16 09:38 AM (7 years, 3 months ago)

And then I put the dried spores without any water on the sterile agar plate?

(I will directly read some tutorials to that topic).


Does your SAB has gloves implemented or just two holes to get your hands/arms inside?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: B+ Growkit, first flush great, second flush with contamination? [Re: AlCapone2k]
    #23712597 - 10/06/16 09:40 AM (7 years, 3 months ago)

Just two holes. Like this:



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How to shroomery like a pro! (Seriously, everyone read this!)
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OfflineMushierage
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Re: B+ Growkit, first flush great, second flush with contamination? [Re: Mad Season]
    #23712621 - 10/06/16 09:46 AM (7 years, 3 months ago)

The purpose of the SAB is to just create an environment without draft or moving air.  Adding gloves is just going to cause positive pressure inside and that air will stir up as it finds the smallest hole in your box to exit.  That's why you use 6 inch holes and not "sealed ones".

Just set up your SAB and let the air be still in the entire room you are using it in for about an hour.  No fans, no Ac, no open windows.  It'll work great. 

As far as spores, you can drag a sterile swap or inoculation loop on a print very briefly and then drag it across your agar plate.  If you're trying to isolate from just bacteria, just drag a "z" like shape and wait a few days.  Take the first sign of healthy Myc you can find and transfer it, making sure not to transfer bacteria with it.  Don't wait til your plate grows all the way out, unless you have already transferred and made your culture clean.  The smaller the culture has grown out, the less chance you'll be transferring bacteria with your culture.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



Edited by Mushierage (10/06/16 12:14 PM)


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