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xThunder
Stranger


Registered: 07/03/12
Posts: 83
Last seen: 3 years, 8 months
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First time PF tek help, pins in jar before full colonization?
#23711873 - 10/06/16 12:03 AM (7 years, 4 months ago) |
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Hey guys, so after months of consideration, me and a friend of mine decided to take a shot at the classic roger rabit PF tek that everyone knows and loves. I'm not going to bother with the details from the start of the process because it was all pretty standard. verm+BRF+water goes into jars, sterilize jars, inoculate in a sterilized chamber all that jazz.
So for our first few attempts we decided to just do two seperate full pint jars(in the vid roger uses half pint jars I believe) and we adjusted all our amounts for substrate and inoculation accordingly. This was just meant to be a test run so we could get a feel for this hobby. Our first attempt failed, we got hit with the forest green mold in both jars and came to the conclusion that our inoculation likely wasn't sterile enough.
Second attempt,we waited for a couple weeks and didn't notice any kind of mycelium growth. We realized that we foolishly inoculated shortly after sterilizing the jars with steam so nothing really happened, they just died in the heat...or so we thought. I just left the failed attempts in my closet for a while(not the green mold ones, i disposed of those of course) and hadn't checked on them because I figured there was no point. So, last week I made the decision to get rid of them finally, one jar did in fact not colonize at all....however, one of them had surprisingly grown quite a bit mycelium!
Not only was it colonized, but little tiny pins of mushrooms could be seen in various parts of the jar...but there were still patches of uncolonized verm in other parts of the jar. Roger was very specific in the video that the ENTIRE jar had to be colonized before you could fruit, so I waited a few more days and here is what I'm currently working with.
I'm just going to gyazo the pics I uploaded to my computer because I'm not sure of better way to do it lol. These are all of the same jar but from each of the 4 sides so you can see exactly what I mean.
Pic #1- https://gyazo.com/645e39fd6aaa1337dff30876470326f3
Pic #2- https://gyazo.com/69929f5adb81e613ebe4804a7eeab3d6
Pic #3- https://gyazo.com/e3abda2dbe8d655202340f33f19e3701
Pic #4- https://gyazo.com/e95acc5ac4578347617c510d5ef8235a
Ok, so I have quite a few questions here. I know its just a single jar but this is my first time growing and I'm mostly coming at this as a learning experience so I'm making a big deal out of it.
1. How the hell did this happen, is it normal for the cakes to fruit so much within the jar? Especially in pic #2 you can see how big these things are getting in there, yet in the first and final pic you can see that there is still uncolonized verm. I'm not sure exactly what question I'm asking here...what is going on/happened?
2. What do I do from here? I know the next step is the dunk n roll, but is there really a reason for me to do that at this point? It's literally already fruiting, so will I gain anything from the dunk and roll? also it just seems like it would be weird to do that with an already fruiting cake and leave it soaking for a night etc.
3. Is this bad necessesarily? If so, why? Will how the jar ended up colonizing affect the potency at all, or something worse that I should be aware of?(contaminants etc)
4. Aside from all of this, how does the jar look? Again this is my first time and I really don't have much of an eye for it yet, I dont think there are any contaminants or molds but I'm not 100% sure, I trust your guys' eyes much more on this.
I have a tub and perlite ready to go for fruiting, I just have to make the holes and I could have this thing going. Should this be my next step? How would you guys proceed from here?
Thank you for any advice, again I'm just trying to learn from this experience for future grows. I apologize if these are noob questions!
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: First time PF tek help, pins in jar before full colonization? [Re: xThunder]
#23712002 - 10/06/16 01:48 AM (7 years, 4 months ago) |
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..... Seriously.... No one is gonna read all that simply to cherry pick your questions.
Also, upload your pics through the shroomery, offsite links aren't safe for us to click.
But to answer the question in your subject, generally when a jar pins before full colonization it means that it's contaminated with bacteria. The uncolonized parts have actually been colonized by the bacteria and I'm sure you will be able to see the mycelium reacting to it. You can take the cake out, cut off the uncolonized part, rinse it well and then dunk, roll and fruit.
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xThunder
Stranger


Registered: 07/03/12
Posts: 83
Last seen: 3 years, 8 months
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Re: First time PF tek help, pins in jar before full colonization? [Re: Supalemonhaze]
#23712033 - 10/06/16 02:30 AM (7 years, 4 months ago) |
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Quote:
Supalemonhaze said:
..... Seriously.... No one is gonna read all that simply to cherry pick your questions.
Also, upload your pics through the shroomery, offsite links aren't safe for us to click.
But to answer the question in your subject, generally when a jar pins before full colonization it means that it's contaminated with bacteria. The uncolonized parts have actually been colonized by the bacteria and I'm sure you will be able to see the mycelium reacting to it. You can take the cake out, cut off the uncolonized part, rinse it well and then dunk, roll and fruit.
ouch, sorry about that, just wanted to be thorough 
I knew there was a way to do it with the pics but I figured gyazo was familiar enough, I shouldn't have been so lazy. I know for next time now though. I'd edit it in but you already answered my question quite well.
Thank you for your response, that was very helpful. I'll try that and see how it goes.
Edited by xThunder (10/06/16 02:34 AM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: First time PF tek help, pins in jar before full colonization? [Re: xThunder]
#23712043 - 10/06/16 02:43 AM (7 years, 4 months ago) |
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Dang, I'm psychic. 
Goodluck with your cake man
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