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acidninja
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Update: Here We Go Again . . .
#23710020 - 10/05/16 01:34 PM (7 years, 3 months ago) |
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So I'm managed to use up all the BRF from my last project so I could only make 2 jars (2 jars cause im playing it safe), all of my last jars were a total cluster fuck due to open air, i did all the right sterilization: gloves, mask, in the bathroom with disinfectant pray, cook in steam for 90 mins (following rr's method) and keep them on a shelf 2m from the floor with airflow and ambient light, id also dised my room before to, anyway, here's the finished product, I'm keeping these 2 in filter bags sitting at around 70-75 in my "incubator" (a tub made of tin foil and a desk lamp covered with yellow paper(all dised)
pretty proud of it tbh. 
I'll be on with future updates!
Thanks AJ

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Edited by acidninja (10/05/16 01:48 PM)
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mushboy
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Re: Update: Here We Go Again . . . [Re: acidninja]
#23710044 - 10/05/16 01:39 PM (7 years, 3 months ago) |
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i doubt your past failure was due to colonizing in open air.
can you detail your approach so people can better inform you?
why are you plugging your amazon wishlist. i dont want to know what you shop for. nor should you be ok with me knowing your shopping trends.
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acidninja
student



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Re: Update: Here We Go Again . . . [Re: mushboy]
#23710055 - 10/05/16 01:44 PM (7 years, 3 months ago) |
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edited main thread*
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acidninja
student



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Re: Update: Here We Go Again . . . [Re: acidninja]
#23710194 - 10/05/16 02:46 PM (7 years, 3 months ago) |
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Also the light is on a 12 hour cycle
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mushboy
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Re: Update: Here We Go Again . . . [Re: acidninja]
#23710197 - 10/05/16 02:48 PM (7 years, 3 months ago) |
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build a still air box.
do not knock up jars in a room where you shit, piss, brush teeth, beat off, shower.
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acidninja
student



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Re: Update: Here We Go Again . . . [Re: mushboy]
#23710200 - 10/05/16 02:49 PM (7 years, 3 months ago) |
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to late really, anyway, i bleached out the whole bathroom and left it for 3 hours with ac on.
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mushboy
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Re: Update: Here We Go Again . . . [Re: acidninja]
#23710236 - 10/05/16 03:01 PM (7 years, 3 months ago) |
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Quote:
TheAcidJunky said: to late really, anyway, i bleached out the whole bathroom and left it for 3 hours with ac on.
bleaching is ok.
ac on for 3 hours is not.
no space/area is going to be sterile unless you have a sealed off lab with a moonsuit. the concept of the SAB(still air box) is NO AIR CURRENT. i get your mindset but the last thing you want is air blowing around.
you want still air. as still as inside a plastic tote with arm holes drilled in it. also you spray the inside of the box with soapy water so contams stick to the walls and not float around landing on the needle you just flamed..
and its not too late!! make more jars. always make more jars. keep improving
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acidninja
student



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Re: Update: Here We Go Again . . . [Re: acidninja]
#23710250 - 10/05/16 03:05 PM (7 years, 3 months ago) |
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yer im getting supplies tomorrow, btw, i did seal the bottom of the bathroom door and ac fan sucks air out, its doesn't blow it in and around
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mushboy
modboy



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Re: Update: Here We Go Again . . . [Re: acidninja]
#23710259 - 10/05/16 03:07 PM (7 years, 3 months ago) |
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right but the sucking of air can land ickys on your mushingtons.
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acidninja
student



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Re: Update: Here We Go Again . . . [Re: mushboy]
#23710288 - 10/05/16 03:18 PM (7 years, 3 months ago) |
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ooooooooooooooooooh righto, gottem, il be makin a air stopper tub thing tomoz den lel
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jesustripped
weirdy


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Re: Update: Here We Go Again . . . [Re: acidninja]
#23710298 - 10/05/16 03:21 PM (7 years, 3 months ago) |
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They don't need to be in bags. They would probably prefer not to be in the bags.
-------------------- row row row your boat gently down the stream, merrily merrily merrily merrily life is but a dream
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mushboy
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Re: Update: Here We Go Again . . . [Re: acidninja]
#23710299 - 10/05/16 03:21 PM (7 years, 3 months ago) |
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they dont have to be fancy either...
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Mushierage
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Re: Update: Here We Go Again . . . [Re: mushboy]
#23710743 - 10/05/16 06:03 PM (7 years, 3 months ago) |
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This is how my jars colonize.

They aren't PFtek obviously but the same concept applies. Any bacterial problems you might be having is due to either your sterile inoculation techniques or more likely, your prep and substrate sterilization techniques.
PF should be balls easy if you are at least PCing and prepping correctly.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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mushboy
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Re: Update: Here We Go Again . . . [Re: Mushierage]
#23710761 - 10/05/16 06:08 PM (7 years, 3 months ago) |
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omg tin foil over 2 jars in the back!? like bruh...
joking.
i love out in the open jars. makes the grow more ...casual? much nicer than doing it in the bedroom. even if the parents were cool with it.. hard to get your mom to mist your cakes
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Mushierage
SWIM Sinker



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Re: Update: Here We Go Again . . . [Re: mushboy]
#23710792 - 10/05/16 06:15 PM (7 years, 3 months ago) |
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Shuddap. I'm g2ging tonight.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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mushboy
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Re: Update: Here We Go Again . . . [Re: Mushierage]
#23711144 - 10/05/16 07:37 PM (7 years, 3 months ago) |
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acidninja
student



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Re: Update: Here We Go Again . . . [Re: jesustripped]
#23713412 - 10/06/16 02:43 PM (7 years, 3 months ago) |
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Quote:
jesustripped said: They don't need to be in bags. They would probably prefer not to be in the bags.
tbh im really not taking any chances this time, i dont care how long it takes, i shake the bags for fresh air at least 3 times a day, im working on a still air box right now anyway
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JacobStorm
psychedelic cartel



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Re: Update: Here We Go Again . . . [Re: acidninja]
#23713481 - 10/06/16 03:02 PM (7 years, 3 months ago) |
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You shake the bags for fresh air? This probably isnt going to end well for you.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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JacobStorm
psychedelic cartel



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Re: Update: Here We Go Again . . . [Re: JacobStorm]
#23713483 - 10/06/16 03:03 PM (7 years, 3 months ago) |
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Were probably not going to be seeing you in the near future
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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mrmazdarx9
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Re: Update: Here We Go Again . . . [Re: JacobStorm]
#23713492 - 10/06/16 03:07 PM (7 years, 3 months ago) |
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We keep telling how to do it then you add a modification that will only fuck it up. The still moist warm air in the bag will probably knacker it up. Just follow the tek and you'll succeed, you can't reinvent the wheel.
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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mushboy
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Re: Update: Here We Go Again . . . [Re: acidninja]
#23713494 - 10/06/16 03:08 PM (7 years, 3 months ago) |
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Quote:
jesustripped said: ... i shake the bags for fresh air at least 3 times a day,
thats a new one.
leave the bags alone.
props on making a SAB
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JacobStorm
psychedelic cartel



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Re: Update: Here We Go Again . . . [Re: mushboy]
#23713502 - 10/06/16 03:11 PM (7 years, 3 months ago) |
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Shaking the bag will only break apart the microscopic fibers of the mycelium it then will have to work very hard to recover. 3 times a day? That BS right there will never colonize.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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MushroomBilly
Explorer


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Re: Update: Here We Go Again . . . [Re: acidninja]
#23713532 - 10/06/16 03:20 PM (7 years, 3 months ago) |
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I haven't read all the posts here but what I would say is that I've been inoculating rye for a few years now (and cakes in the early days) in open air after washing my hands and spraying a little Oust and only had one contamination on one jar. Usually do grain to grain, just crack the lid, pour a little and seal up quick. If Quote:
TheAcidJunky said:
Quote:
jesustripped said: They don't need to be in bags. They would probably prefer not to be in the bags.
tbh im really not taking any chances this time, i dont care how long it takes, i shake the bags for fresh air at least 3 times a day, im working on a still air box right now anyway
I'm sure people will jump all over me here but I've been inoculating rye for a few years now (and cakes in the early days) in open air after washing my hands and spraying a little Oust and only had one contamination on one jar. Usually do grain to grain still in the pressure cooker once cooled, just crack the lid, pour a little and seal up quick. I have a SAB for agar but rarely use it. Perhaps there is something wrong with your pressure cooker if you're experiencing contamination at a level enough to warrant all these extra measures??
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mrmazdarx9
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Quote:
MushroomBilly said: I haven't read all the posts here but what I would say is that I've been inoculating rye for a few years now (and cakes in the early days) in open air after washing my hands and spraying a little Oust and only had one contamination on one jar. Usually do grain to grain, just crack the lid, pour a little and seal up quick. If Quote:
TheAcidJunky said:
Quote:
jesustripped said: They don't need to be in bags. They would probably prefer not to be in the bags.
tbh im really not taking any chances this time, i dont care how long it takes, i shake the bags for fresh air at least 3 times a day, im working on a still air box right now anyway
I'm sure people will jump all over me here but I've been inoculating rye for a few years now (and cakes in the early days) in open air after washing my hands and spraying a little Oust and only had one contamination on one jar. Usually do grain to grain still in the pressure cooker once cooled, just crack the lid, pour a little and seal up quick. I have a SAB for agar but rarely use it. Perhaps there is something wrong with your pressure cooker if you're experiencing contamination at a level enough to warrant all these extra measures??
Why did you post the same thing twice in on post?
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Quote:
MushroomBilly said:
I'm sure people will jump all over me here but I've been inoculating rye for a few years now (and cakes in the early days) in open air after washing my hands and spraying a little Oust and only had one contamination on one jar. Usually do grain to grain still in the pressure cooker once cooled, just crack the lid, pour a little and seal up quick. I have a SAB for agar but rarely use it. Perhaps there is something wrong with your pressure cooker if you're experiencing contamination at a level enough to warrant all these extra measures??
Anything that involves the needle and tiny hole I OAI Never had a problem.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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mushboy
modboy



Registered: 04/24/05
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Quote:
MushroomBilly said: I haven't read all the posts here but what I would say is that I've been inoculating rye for a few years now (and cakes in the early days) in open air after washing my hands and spraying a little Oust and only had one contamination on one jar. Usually do grain to grain, just crack the lid, pour a little and seal up quick. If Quote:
TheAcidJunky said:
Quote:
jesustripped said: They don't need to be in bags. They would probably prefer not to be in the bags.
tbh im really not taking any chances this time, i dont care how long it takes, i shake the bags for fresh air at least 3 times a day, im working on a still air box right now anyway
I'm sure people will jump all over me here but I've been inoculating rye for a few years now (and cakes in the early days) in open air after washing my hands and spraying a little Oust and only had one contamination on one jar. Usually do grain to grain still in the pressure cooker once cooled, just crack the lid, pour a little and seal up quick. I have a SAB for agar but rarely use it. Perhaps there is something wrong with your pressure cooker if you're experiencing contamination at a level enough to warrant all these extra measures??
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jesustripped
weirdy


Registered: 02/26/14
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Re: Update: Here We Go Again . . . [Re: mushboy]
#23713822 - 10/06/16 05:05 PM (7 years, 3 months ago) |
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Quote:
mushboy said:
Quote:
jesustripped said: ... i shake the bags for fresh air at least 3 times a day,
thats a new one.
leave the bags alone.
props on making a SAB
Please nobody else attribute this quote to me. P!ease.
-------------------- row row row your boat gently down the stream, merrily merrily merrily merrily life is but a dream
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Mushierage
SWIM Sinker



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Re: Update: Here We Go Again . . . [Re: jesustripped]
#23713871 - 10/06/16 05:25 PM (7 years, 3 months ago) |
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But then I wouldn't be able to use this gem...
Quote:
mushboy said:
I am a butt pirate.
I joke I keed.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Kenetic
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Re: Update: Here We Go Again . . . [Re: Mushierage]
#23713881 - 10/06/16 05:31 PM (7 years, 3 months ago) |
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If he's a butt pirate then bodhisatta is the booty lol
Sorry bod the vape talk made you gay by proxy
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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mushboy
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Re: Update: Here We Go Again . . . [Re: Kenetic]
#23714013 - 10/06/16 06:24 PM (7 years, 3 months ago) |
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argh!!!
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acidninja
student



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Re: Update: Here We Go Again . . . [Re: mushboy]
#23714888 - 10/07/16 12:43 AM (7 years, 3 months ago) |
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Alright ive taken the bags of but i swear to god if anymore shit like that happens again in those jars im just giving up
--------------------
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acidninja
student



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Re: Update: Here We Go Again . . . [Re: acidninja]
#23714902 - 10/07/16 12:55 AM (7 years, 3 months ago) |
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Infact no, theres no point, im going to wait for growth, stop shaking the bags and then if there is any contams once i take the jars out i can atleast save it
--------------------
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JacobStorm
psychedelic cartel



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Re: Update: Here We Go Again . . . [Re: acidninja]
#23715215 - 10/07/16 06:05 AM (7 years, 3 months ago) |
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Save it? Wouldnt it be less work to just follow the tek and do it right?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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mrmazdarx9
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Re: Update: Here We Go Again . . . [Re: JacobStorm]
#23715793 - 10/07/16 09:51 AM (7 years, 3 months ago) |
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If there are any contams in your jar you can't save it. It goes in the bin, if you open it you will spread that shit through out your house or where ever.
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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acidninja
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Ok so ive removed the jars out of the bags in my sterile still box i made yesterday and then took them out and placed them in my re sterilized incubation area. the inoc holes have no protection and the only protection the sub has in the 2cm of dry verm on top of the sub in the jars, im still really tempted to put micro pore on the holes but are there any suggestions?
--------------------
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Edited by acidninja (10/07/16 10:24 AM)
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dankington
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Quote:
TheAcidJunky said: Ok so ive removed the jars out of the bags in my sterile still box i made yesterday and then took them out and placed them in my re sterilized incubation area. the inoc holes have no protection and the only protection the sub has in the 2cm of dry verm on top of the sub in the jars, im still really tempted to put micro pore on the holes but are there any suggestions?

WTF happened in this thread??? This last post is all that I can really even understand. Jesus shaking sacks and shit, what's going on? 
Anyways, you can put micropore on your inoc. point holes. It won't hurt anything. Just leave those jars out, 2cm of dry verm at the top of the jars sounds about right
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JacobStorm
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There are 2.5CM to an inch. I dont think 2CM is enough. OP just dont fuck with these. Forget about them. Dont even touch them. You run the risk of any bactiria that has gotten on the 2CM of dry verm, falling down into your cake.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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mrmazdarx9
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I basically fill mine with verm brf to the threaded bit of the jar then the rest dry verm to the lip never had a problem, and looks like the exact jars I use.
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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JacobStorm
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I hope this works out I do. But I dont think its gonna be great.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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SloppyJoseph
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Re: Update: Here We Go Again . . . [Re: mushboy]
#23716691 - 10/07/16 03:16 PM (7 years, 3 months ago) |
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Quote:
mushboy said: they dont have to be fancy either...


I love it
-------------------- AMU Q&A
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MushroomBilly
Explorer


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Quote:
JacobStorm said: There are 2.5CM to an inch. I dont think 2CM is enough. OP just dont fuck with these. Forget about them. Dont even touch them. You run the risk of any bactiria that has gotten on the 2CM of dry verm, falling down into your cake.

This was what concerned me about shaking the bags. Just leave them be - competitors may well and probably will get in but the verm acts as a filter - you disturb it and you run the risk of it getting at the good stuff you intended for the mycelium
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acidninja
student



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alright leave em be now, constant ambient light, thin micropore over inoc holes and sittin at 68-70f constant
--------------------
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mrmazdarx9
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Re: Update: Here We Go Again . . . [Re: acidninja]
#23718412 - 10/08/16 03:52 AM (7 years, 3 months ago) |
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That'll do mate
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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utestew
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Re: Update: Here We Go Again . . . [Re: mushboy]
#23761540 - 10/22/16 05:15 PM (7 years, 3 months ago) |
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about what mine looks like haha oops..
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